We narrowed to 11,492 results for: ANG

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xCas12i

Plasmid

#195333

Purpose

vector for encoding a human codon-optimized xCas12i driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter

Depositor

Yingsi Zhou

Article

Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

Insert

human codon-optimized xCas12i

Tags

3xFlag

Expression

Mammalian

Promoter

CBh, CMV, hU6

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-134

Plasmid

#118520

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mOrange2

Use

Minitn5 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pRSET a MatryoshCaMP6s

Plasmid

#100019

Purpose

Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.

Depositor

Wolf Frommer

Article

Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

Insert

MatryoshCaMP6s

Tags

6x HIS tag and Xpress_EK tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pICH-nuc-roGFP2-ORP1

Plasmid

#191758

Purpose

Ti plasmid pICH86988 harbouring nuc-roGFP2-ORP1 for peroxide detection in the nuclei of plant cells.

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

Insert

nuc-roGFP2-ORP1

Use

Binary agrobacterium ti plasmid

Expression

Plant

Promoter

CaMV 35S

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pICH-nuc-roGFP2-synORP1

Plasmid

#191759

Purpose

Ti plasmid pICH86988 harbouring nuc-roGFP2-synORP1 for H2O2 detection in the nuclei of plant cells.

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

Insert

nuc-roGFP2-synORP1

Use

Binary agrobacterium ti plasmid

Expression

Plant

Promoter

CaMV 35S

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 NSP10_nostop

Plasmid

#149313

Purpose

Gateway-compatible Entry vector, with insert of NSP10 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP10 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 SARS-CoV-2 NSP4_nostop

Plasmid

#149307

Purpose

Gateway-compatible Entry vector, with insert of NSP4 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP4 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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LentiV_Neo_SIK3_CR

Plasmid

#108104

Purpose

Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation) with neomycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Insert

SIK3 (SIK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

change guanine 501 to cytosine (silent mutation)

Promoter

EFS promoter

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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EIF2AK3 gRNA (BRDN0001145848)

Plasmid

#77166

Purpose

3rd generation lentiviral gRNA plasmid targeting human EIF2AK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EIF2AK3 (EIF2AK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pTK52_GFP-NuMA-C-3A

Plasmid

#46350

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

NuMA C-terminal fragment (NUMA1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

C-terminal fragment from 1700-2115, and changed T…

Promoter

pBABE

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pMSCV FHA-Stop

Plasmid

#27050

Depositor

Jayanta Debnath

Article

Radoshevich et al Cell. 2010 Aug 20. 142(4):590-600.

Insert

autophagy-related 12 (yeast) (Atg12 Mouse)

Use

Retroviral

Tags

HA and flag

Expression

Mammalian

Mutation

changed glycine 141 to stop codon.

Available Since

Jan. 13, 2011

Availability

Academic Institutions and Nonprofits only
CLM RP HTR

Plasmid

#24430

Depositor

Ron Vale

Article

Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

Insert

Kinesin (KIF5B Human)

Tags

6XHis

Expression

Bacterial

Mutation

C7S/C65A/C168A/C174S/C294A/C330S/C421A

Available Since

June 30, 2010

Availability

Academic Institutions and Nonprofits only
LB595 (Bcl-2 promoter CRE mut)

Plasmid

#15243

Depositor

Linda Boxer

Article

Heckman et al Oncogene. 2002 May 30. 21(24):3898-908.

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial

Mutation

Mutated CRE site at -1537 changed from GTGACGTTA …

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
pMXs-EGFP-Rheb SSVM-IP

Plasmid

#13832

Depositor

Shinya Yamanaka

Article

Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

Insert

Rheb SSVM (Rheb Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

C181 changed to serine

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
pConRef-2H12

Plasmid

#221182

Purpose

cdGreen expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11213)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Inserts

cdGreen
mScarlet-I

Expression

Bacterial

Mutation

N-terminus changed: starts with MSKKYGEAVIKE ...

Promoter

Constitutive and None (same as cdGreen)

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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YFP(1-158)-STOP

Plasmid

#66107

Purpose

This vector attaches YFP(1-158) to the C-terminus of a protein

Depositor

Catherine Berlot

Article

Hu et al J Biol Chem. 2013 Apr 19;288(16):11416-3

Insert

YFP(1-158)STOP

Expression

Mammalian

Mutation

Gln69 was changed to Met.

Promoter

CMV

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pRSFDuet1_CoiA1_CoiB

Plasmid

#208761

Purpose

Precursor peptide CoiA1 and synthase CoiB, for the co-expression of CoiA1 in E.coli.

Depositor

Wilfred van der Donk

Article

Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

Inserts

CoiA1
CoiB

Tags

Hisx6 Tag and SUMO

Expression

Bacterial

Mutation

Changed Ser 20 to Asn, Changed Arg 84 to Lys, bot…

Promoter

T7

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA-S1b (BsaI)-CBh-eCas12f1

Plasmid

#233078

Purpose

Expression vector for sgRNA-S1b and eCas12f1

Depositor

Kyoungmi Kim

Article

Park et al Nat Commun. 2025 Jan 15;16(1):677. doi:

Insert

U6-sgRNA-S1b (BsaI)-CBh-bpNLS-eCas12f1-2xFLAG-P2A-EGFP

Use

CRISPR

Tags

2xFLAG

Expression

Mammalian

Promoter

hU6, CBh

Available Since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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p5E-3x125bp-mnx1

Plasmid

#169043

Purpose

5' entry vector for Gateway cloning for expression in spinal motor neurons using 3 tandem repeats of the zebrafish mnx1 enhancer.

Depositor

Paola Arlotta

Article

Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

Insert

Mnx1 enhancer (3x) (mnx1 Zebrafish)

Use

Gateway entry cloning vector

Promoter

3xMnx1

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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ePB-PURO-FLAG-METTL3-APPA

Plasmid

#160253

Purpose

For stable integration of catalytic inactive FLAG-METTL3 in human cells with trasposase

Depositor

Alessandro Fatica

Article

Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

Insert

methyltransferase like 3 (METTL3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changes in aa 395–398, DPPW → APPA

Promoter

tight TRE promoter

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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