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pHygromycin (GB0211)

Plasmid

#68204

Purpose

Provides the CDS of the Hygromycin resistance gene as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Hygromycin resistance gene coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only

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WFS1 pEGFP-N2 (1472)

Plasmid

#62051

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

WFS1 (WFS1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
RHBDD1 pEGFP-N2 (1078)

Plasmid

#62028

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

RHBDD1 (RHBDD1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only

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TAL2322

Plasmid

#36748

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FOXO1-TALEN_Left (FOXO1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
TAL2284

Plasmid

#36719

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

NCOR2-TALEN_Left (Ncor2 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pLKO-tet-puro-sh-ex16

Plasmid

#35167

Depositor

Benjamin Blencowe

Article

Gabut et al Cell. 2011 Sep 30;147(1):132-46. Epub 20

Insert

Foxp1 (Foxp1 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pmCherryC1 VAP-B

Plasmid

#226409

Purpose

Expression of human VAP-B fused to mCherry in mammalian cells

Depositor

Catherine Tomasetto

Article

Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

Insert

VAP-B (VAPB Human)

Tags

HA, T7-Xpress tag, and mCherry

Expression

Mammalian

Available Since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CD86

Plasmid

#223604

Purpose

Lentiviral expression of human CD86 in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD86 (CD86 Human)

Use

Lentiviral; mammalian expression

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pFlp-Km

Plasmid

#222334

Purpose

Expresses Flp and kanamycin to enable excision of antibiotic resistance cassettes; flp-cl-sacB-pL1V-Lv1-neo-pBBR1-ELT3

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Inserts

sacB (sacB B. subtilis)
flp (flp Budding Yeast)
cI (cI )

Promoter

cl, flp, and sacB

Available Since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pDisplay-EGFP-CAAX

Plasmid

#208683

Purpose

Expresses the membrane-localized enhanced green fluorescent protein EGFP-CAAX in mammalian cells

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

membrane-localized enhanced green fluorescent protein EGFP-CAAX

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCAX-EGFP-CIP4

Plasmid

#178363

Purpose

Expresses CIP4 c-terminal labeled with EGFP in mammalian cells

Depositor

Erik Dent

Article

Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

Insert

Cdc42 Interacting Protein 4 (TRIP10 Human)

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCMU-4

Plasmid

#172353

Purpose

CRISPR gene editing in Streptomyces, codon-optimized Cas9 behind theophylline riboswitch and D4 promoter, with custom gRNA

Depositor

Eriko Takano

Article

Ye et al J Ind Microbiol Biotechnol. 2020 May;47(

Insert

D4-TheoR-cas9

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

D4

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-Trib3

Plasmid

#131157

Purpose

Mammalian expression of flag-tagged mouse TRIB3

Depositor

Karen Keeshan, Warren Pear

Article

Dedhia et al Blood. 2010 Aug 26;116(8):1321-8. doi: 1

Insert

Trib3 (Trib3 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA6-N-3XFLAG-Ubxd8

Plasmid

#123599

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

Fas associated factor family member 2 (Faf2 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a- mEGFP-kanMX6

Plasmid

#105146

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

mEGFP-kanMX6

Tags

mEGFP

Expression

Bacterial and Yeast

Mutation

A206K

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-Cx43

Plasmid

#60691

Purpose

mammalian expression of rat Cx43

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
pCMB-TMEr

Plasmid

#61208

Purpose

Fluorescent reporter for plasma membrane and peri-arbuscular membrane MtBCPsp-mCherry-BCP expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtBCPsp-mCherry-BCP

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

April 10, 2015

Availability

Academic Institutions and Nonprofits only

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TAL2451

Plasmid

#36840

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

HMGA2-TALEN_Right (Hmga2 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2237

Plasmid

#36688

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

CHD4-TALEN_Right (Chd4 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2268

Plasmid

#36705

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FOXO3-TALEN_Left (Foxo3 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only