We narrowed to 16,834 results for: Emb;

1. pGEMHE:hTRAAK(Q258K)

   Plasmid

   #133077

   Purpose

   X. laevis expression vector. It will generate the human TRAAK channel

   Depositor

   Dan Minor

   Article

   Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

   Insert

   Potassium channel subfamily K member 2 (KCNK2, TREK-1), Q258K (Kcnk2 Mouse)
   

   Use

   Tags

   Expression

   Mutation

   Q258K

   Promoter

   T7

   Available since

   Oct. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-mCherry-T2A-miniSOG-C-Far

   Plasmid

   #193915

   Purpose

   Expresses miniSOG targeted to the plasma membrane of neurons, joined by a T2A cleavage site with mCherry; for AAV production

   Depositor

   Xiao Wang

   Article

   Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

   Insert

   miniSOG
   

   Use

   AAV

   Tags

   Farnesylation signal sequence and Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMM2-rPMCA3b

   Plasmid

   #47763

   Purpose

   mammalian expression of rat PMCA3b

   Depositor

   Emanuel Strehler, John Penniston

   Article

   Enyedi et al J Biol Chem. 1997 Oct 31;272(44):27525-8

   Insert

   PMCA3b (Atp2b3 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Sept. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pCDH-AtAFB2-mTagBFP2

   Plasmid

   #179889

   Purpose

   Expresses AtAFB2 (auxin-inducible degron system) fused to mTagBFP2 (lentiviral plasmid)

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   AtAFB2 (AFB2 Mustard Weed, Synthetic)
   

   Use

   Lentiviral

   Tags

   mTagBFP2

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EF1

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPBpuro-mNG-eDHFR(69K6)-p85iSH2

   Plasmid

   #172103

   Purpose

   Mammalian expression of p85iSH2 fused to mNeonGreen-eDHFR(69K6)

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   mNeonGreen-eDHFR(69K6)-p85iSH2 (Pik3r2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV.U6gT28.13.EFS-NS.H2B-RFP

   Plasmid

   #170388

   Purpose

   Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 13. Expressing nuclear RFP.

   Depositor

   Johan Jakobsson

   Article

   Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

   Insert

   H2B-RFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EFS-NS

   Available since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSUPERpuro-BglII-Hs4E-T_AC

   Plasmid

   #148445

   Purpose

   Mammalian Expression of Hs4E-T-shRNA

   Depositor

   Elisa Izaurralde

   Article

   Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

   Insert

   Hs4E-T-shRNA (EIF4ENIF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. WFS1 mRFP-N2 (891)

   Plasmid

   #62018

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

   Insert

   WFS1 (WFS1 Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA5FRT/TO-UBE2QL1-GFP

   Plasmid

   #124667

   Purpose

   Expresses UBE2QL1 with C-terminal GFP tag in mammalian cells

   Depositor

   Hemmo Meyer

   Article

   Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

   Insert

   UBE2QL1 (UBE2QL1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMiNGo11-3

    Plasmid

    #126058

    Purpose

    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Inserts

    mitochondria Rieske iron sulphur protein (1-100 aa)
    ccdB cassette (BsaI)
    GFP11x3 (5 aa linker)
    

    Use

    Binary

    Tags

    GFP1-10

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMiNGo11-7

    Plasmid

    #126057

    Purpose

    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Inserts

    mitochondria Rieske iron sulphur protein (1-100 aa)
    ccdB cassette (BsaI)
    GFP11x7 (5 aa linker)
    

    Use

    Binary

    Tags

    GFP1-10

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. NET4 pEGFP-N2 (1201)

    Plasmid

    #62041

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al PLoS One. 2011 Apr 14;6(4):e18762. doi:

    Insert

    Net4 (TMEM53 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pAV113.loxP

    Plasmid

    #63186

    Purpose

    pRS413 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAV115.loxP

    Plasmid

    #63187

    Purpose

    pRS415 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAV116.loxP

    Plasmid

    #63188

    Purpose

    pRS416 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTH743-CEN-RLuc/slowstaCFLuc

    Plasmid

    #38223

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    The last three codons of the full-length Firefly …

    Promoter

    ADH1 and TDH3 (=GPD)

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. ER-frNFAST

    Plasmid

    #233599

    Purpose

    Expression of frNFAST on the ER membrane

    Depositor

    Riccardo Filadi

    Article

    Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

    Insert

    frNFAST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. Start Stuffer TurboID V5 T2A BFP

    Plasmid

    #233257

    Purpose

    Expression of BFP-tagged vector for Turbo ID experiments

    Depositor

    David Bryant

    Article

    Nikolatou et al EMBO J. 2023 Sep 18;42(18):e113987. doi:

    Insert

    TurboID V5 T2A BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBeta-MHC-promoter DV (JDW 26)

    Plasmid

    #229835

    Purpose

    A gateway compatible destination vector containing the murine beta-MHC promoter for embryonic and postnatal cardiomyocyte expression.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pXMLC2-DEST (JDW 7)

    Plasmid

    #229833

    Purpose

    A gateway compatible destination vector containing the Xenopous myosin light chain 2 promoter for embryonic and adult cardiomyocyte expression.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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