We narrowed to 10,761 results for: T7
-
Plasmid#231870PurposeBacterial expression of N-terminally 6His tagged Gcn4 R2DepositorInsertGcn4 R2
Tags6xHis, TEV cleavage siteExpressionBacterialMutationK118R, K140RPromoterT7Available SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 L123A+
Plasmid#231869PurposeBacterial expression of N-terminally 6His tagged Gcn4 L123A+DepositorInsertGcn4 L123A+
Tags6xHis, TEV cleavage siteExpressionBacterialMutationL123APromoterT7Available SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 Swap2
Plasmid#231865PurposeBacterial expression of N-terminally 6His tagged Gcn4 Swap2DepositorInsertGcn4 Swap2
Tags6xHis, TEV cleavage siteExpressionBacterialPromoterT7Available SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 F124I
Plasmid#231864PurposeBacterial expression of N-terminally 6His tagged Gcn4 F124IDepositorInsertGcn4
Tags6xHis, TEV cleavage siteExpressionBacterialMutationF124IPromoterT7Available SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 Swap3
Plasmid#231860PurposeBacterial expression of N-terminally 6His tagged Gcn4 Swap3DepositorInsertGcn4 Swap3
Tags6xHis, TEV cleavage siteExpressionBacterialPromoterT7Available SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 WT 44mer
Plasmid#231856PurposeBacterial expression of N-terminally 6His tagged Gcn4 WT 44merDepositorInsertGcn4 44mer
Tags6xHis, TEV cleavage siteExpressionBacterialPromoterT7Available SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_FL Gcn4
Plasmid#231855PurposeBacterial expression of N-terminally 6His tagged FL Gcn4DepositorInsertGcn4
Tags6xHis, TEV cleavage siteExpressionBacterialPromoterT7Available SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
Kif3A-EGFP-SNAP
Plasmid#229852PurposeKif3A (aa1-342)-Kif1A neck linker(17aa) fused to Kin1 coiled coil (aa345-406)-EGFP-SNAP-his. This Kif3A was used to link an oligo to the motor using the SNAP tag for single molecule experimentsDepositorAvailable SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLANT-2b
Plasmid#231487PurposeExpresses His6-SUMO^NIT-FraB where "NIT" stands for "no internal translation", reflecting the mutation of the two Shine-Dalgarno-like sequences within the SUMO tag.DepositorInsertsTagsHis6 tag and His6-SUMO^NITExpressionBacterialMutationDeleted amino acids 1-6 (MEPEES). and M83L, E84Q,…PromoterT7 promoterAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
-
Halo-BASU-His
Plasmid#218451PurposeE coli expression vector for Halo-BASU-His biotin ligaseDepositorInsertHaloTag-BASU-His fusion protein
ExpressionBacterialPromoterT7Available SinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
MZB216
Plasmid#221766Purpose"Empty" C-terminal CPD-10xHis vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expressionDepositorInsertrtxA
PromoterT7 PromoterAvailable SinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
E547* FL USP7
Plasmid#229001PurposeE547-mutant of human USP7DepositorInsertUSP7 1-546 (USP7 Human)
Tags6xHis cleavable with thrombinExpressionBacterialMutationE547 mutated to stop codonPromoterT7Available SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVF53
Plasmid#229477Purposeexpresses RssBE229CDepositorInsertRssB (E229C)
ExpressionBacterialMutationE229CPromoterT7Available SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBD1
Plasmid#229463PurposeExpresses RssBDepositorInsertRssB
ExpressionBacterialPromoterT7Available SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVF30
Plasmid#229472PurposeExpresses RssB with R115A mutationDepositorInsertRssB (R115A)
ExpressionBacterialMutationR115APromoterT7Available SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVF29
Plasmid#229473PurposeExpresses RssB with N256A mutationDepositorInsertRssB (N256A)
ExpressionBacterialMutationN256APromoterT7Available SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVF28
Plasmid#229469PurposeExpresses RssB with D104A mutationDepositorInsertRssB (D104A)
ExpressionBacterialMutationD104APromoterT7Available SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVF31
Plasmid#229468PurposeExpresses RssB with Q254A mutationDepositorInsertRssB (Q254A)
ExpressionBacterialMutationQ254APromoterT7Available SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVF16
Plasmid#229465PurposeExpresses RssB with D58P mutationDepositorInsertRssB (D58P)
ExpressionBacterialMutationD58PPromoterT7Available SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMS11
Plasmid#229464PurposeExpresses RssB with His TagDepositorInsertRssB
Tags10xHisExpressionBacterialPromoterT7Available SinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVF14
Plasmid#229455PurposeExpresses IraD with His-tagDepositorInsertIraD
Tags6xHisExpressionBacterialPromoterT7Available SinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVF17
Plasmid#229454PurposeExpresses IraM with His-tagDepositorInsertIraM
Tags6xHisExpressionBacterialPromoterT7Available SinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
K560-Halo-Strep
Plasmid#219681PurposeMammalian expression of KIF5B(1-560)-HaloTag-StrepDepositorInsertKIF5B (KIF5B Human)
TagsHaloTag, Strep tagExpressionMammalianMutation1-560PromoterCMV & T7Available SinceDec. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
KIF5A-SNAPf-HisTag-FLAG
Plasmid#200791PurposeMammalian expression of KIF5A-SNAPf-HisTag-FLAGDepositorAvailable SinceDec. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTYB1-T4gp47
Plasmid#228212PurposeExpression in BL21(DE3) of T4 SbcD-like subunit of palindrome specific endonucleaseDepositorInsert47 (47 Escherichia phage T4)
TagsSce VMA intein-chitin binding domainExpressionBacterialPromoterT7Available SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-MhCINS-6xHis
Plasmid#194304PurposeBacterial expression of MhCINS protein with a C terminal His-tag and transit peptide removed at N terminalDepositorInsertMhCINS
Tags6xHisExpressionBacterialPromoterT7Available SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-hfYFP-TEV-Bcx
Plasmid#191195PurposeFusion of hfYFP and Bacillus circulans xylanase separated by a TEV cleavage site for fluorescence-assisted protein purificationDepositorInserthfYFP & Bcx fusion
Tags6xHis and Bacillus circulans xylanaseExpressionBacterialPromoterT7 promoterAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-hfYFP-TEV-mScarlet-i
Plasmid#191196PurposeFusion of hfYFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purificationDepositorInserthfYFP & mScarlet-I fusion
Tags6xHis and mScarlet-IExpressionBacterialPromoterT7 promoterAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-LSSmGFP-TEV-mScarlet-i
Plasmid#191199PurposeFusion of LSSmGFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purificationDepositorInsertLSSmGFP & mScarlet-I fusion
Tags6xHis and mScarlet-IExpressionBacterialPromoterT7 promoterAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDest17_A1-LCD swap V1
Plasmid#226538PurposeBacterial expression of N-terminally 6His tagged A1-LCD V1DepositorInsertA1-LCD swap V1
Tags6xHisExpressionBacterialPromoterT7Available SinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDest17_A1-LCD swap V2
Plasmid#226539PurposeBacterial expression of N-terminally 6His tagged A1-LCD V2DepositorInsertA1-LCD swap V2
Tags6xHisExpressionBacterialPromoterT7Available SinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDest17_A1-LCD swap V3
Plasmid#226540PurposeBacterial expression of N-terminally 6His tagged A1-LCD V3DepositorInsertA1-LCD swap V3
Tags6xHisExpressionBacterialPromoterT7Available SinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDest17_A1-LCD swap X5
Plasmid#226547PurposeBacterial expression of N-terminally 6His tagged A1-LCD X5DepositorInsertA1-LCD swap X5
Tags6xHisExpressionBacterialPromoterT7Available SinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDest17_A1-LCD swap X7
Plasmid#226549PurposeBacterial expression of N-terminally 6His tagged A1-LCD X7DepositorInsertA1-LCD swap X7
Tags6xHisExpressionBacterialPromoterT7Available SinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDest17_A1-LCD swap X1
Plasmid#226543PurposeBacterial expression of N-terminally 6His tagged A1-LCD X1DepositorInsertA1-LCD swap X1
Tags6xHisExpressionBacterialPromoterT7Available SinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDest17_A1-LCD swap V5
Plasmid#226542PurposeBacterial expression of N-terminally 6His tagged A1-LCD V5DepositorInsertA1-LCD swap V5
Tags6xHisExpressionBacterialPromoterT7Available SinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDest17_A1-LCD swap X4
Plasmid#226546PurposeBacterial expression of N-terminally 6His tagged A1-LCD X4DepositorInsertA1-LCD swap X4
Tags6xHisExpressionBacterialPromoterT7Available SinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDest17_A1-LCD swapX3
Plasmid#226545PurposeBacterial expression of N-terminally 6His tagged A1-LCD X3DepositorInsertA1-LCD swap X3
Tags6xHisExpressionBacterialPromoterT7Available SinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDest17_A1-LCD swap V4
Plasmid#226541PurposeBacterial expression of N-terminally 6His tagged A1-LCD V4DepositorInsertA1-LCD swap V4
Tags6xHisExpressionBacterialPromoterT7Available SinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only