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Plasmid#202845PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC4DepositorInsertEGFP/BC4
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC5-P1A5
Plasmid#202846PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC5DepositorInsertEGFP/BC5
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC7-P1A7
Plasmid#202848PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC7DepositorInsertEGFP/BC7
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRV∆L-5tTA
Plasmid#182966PurposeThe 3rd generation of rabies vector expressing a tetracycline-controlled transactivator (tTA)DepositorInserttTA
UseRabiesTagsExpressionMutationPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX SYT9 C2A
Plasmid#195707PurposeBacterial expression plasmid encoding GST-tagged SYT9 C2A domainDepositorInsertSyt9 (Syt5 Mouse)
UseTagsGSTExpressionBacterialMutationencodes amino acids 124-211 of mouse SYT9PromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX SYT9 C2B
Plasmid#195708PurposeBacterial expression plasmid encoding GST-tagged SYT9 C2B domainDepositorInsertSyt9 (Syt5 Mouse)
UseTagsGSTExpressionBacterialMutationencodes amino acids 253-344 of mouse SYT9PromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX SYT9 C2AB
Plasmid#195709PurposeBacterial expression plasmid encoding GST-tagged SYT9 C2AB domainDepositorInsertSyt9 (Syt5 Mouse)
UseTagsGSTExpressionBacterialMutationencodes amino acids 104-386 of mouse SYT9PromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX SYT1 C2A
Plasmid#195710PurposeBacterial expression plasmid encoding GST-tagged SYT1 C2A domainDepositorInsertSyt1 (Syt1 Rat)
UseTagsGSTExpressionBacterialMutationencodes amino acids 96-264 of mouse SYT1PromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX SYT1 C2B
Plasmid#195711PurposeBacterial expression plasmid encoding GST-tagged SYT1 C2B domainDepositorInsertSyt1 (Syt1 Rat)
UseTagsGSTExpressionBacterialMutationencodes amino acids 248-421 of mouse SYT1PromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX SYT1 C2AB
Plasmid#195712PurposeBacterial expression plasmid encoding GST-tagged SYT1 C2AB domainDepositorInsertSyt1 (Syt1 Rat)
UseTagsGSTExpressionBacterialMutationencodes amino acids 96-421 of mouse SYT1PromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET (SUMO)-Syt9
Plasmid#195713PurposeBacterial expression plasmid encoding SUMO-tagged full-length Syt9DepositorInsertSyt9 (Syt5 Rat)
UseTagsGST-SUMOExpressionBacterialMutationPromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNZ18-CAH
Plasmid#203159PurposeReplicates in E. coli, S. cerevisiae, and A. laidlawiiDepositorInsertYeast centromere, autonomously replicating sequence, histidine auxotrophic marker
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMetTU1-A_Sv5K_araC-PBAD_MCD1_AnaA-T6A-L40A_AnaCNJKFGW_Bba-B0015
Plasmid#202024PurposeExpresses Anabaena flos-aquae ARG cluster (GvpA T6A-L40A mutant) in E. coliDepositorInsertAnabaena flos-aquae ARG cluster (GvpA T6A-L40A mutant)
UseTagsExpressionBacterialMutationGvpA T6A-L40APromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL2084
Plasmid#137726PurposeGateway vector for inducible stem loop RNAi in T.b. brucei 2T1DepositorTypeEmpty backboneUseRNAi; Trypanosoma brucei tet inducible stem loopTagsExpressionMutationPromoterAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCI pHluorin Syt12
Plasmid#184513PurposeMammalian expression of Synaptotagmin 12 with pH-sensitive fluorescent proteinDepositorInsertSynaptotagmin 12 (Syt12 Rat)
UseTagsSuper Ecliptic pHluorinExpressionMammalianMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCI pHluorin Syt17
Plasmid#184514PurposeMammalian expression of Synaptotagmin 17 with pH-sensitive fluorescent proteinDepositorInsertSynaptotagmin 17 (Syt17 Rat)
UseTagsSuper Ecliptic pHluorinExpressionMammalianMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLT237
Plasmid#174300PurposeReplicating plasmid with homology region for introducing PolCC669Y mutation. Confers thiamphenicol resistance.DepositorInsertPolC homology arm
UseTagsExpressionBacterialMutationchanged Cysteine 669 to TyrosinePromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCI pHluorin Syt9
Plasmid#184510PurposeMammalian expression of Synaptotagmin 9 with pH-sensitive fluorescent proteinDepositorInsertSynaptotagmin 9 (Syt9 Mouse)
UseTagsSuper Ecliptic pHluorinExpressionMammalianMutationPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCI pHluorin Syt8
Plasmid#184509PurposeMammalian expression of Synaptotagmin 8 with pH-sensitive fluorescent proteinDepositorInsertSynaptotagmin 8 (Syt8 Mouse)
UseTagsSuper Ecliptic pHluorinExpressionMammalianMutationPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCI pHluorin Syt3
Plasmid#184504PurposeMammalian expression of Synaptotagmin 3 with pH-sensitive fluorescent proteinDepositorInsertSynaptotagmin 3 (Syt3 Rat)
UseTagsSuper Ecliptic pHluorinExpressionMammalianMutationPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
lenti-Cas9-sgHPRT1
Plasmid#196713PurposeCRISPR-KO. WT-SpCas9 and sgRNA targeting HPRT1. Editing-competent cells can be selected with 6-TGDepositorInsertCas9-T2A-BSD-U6-sgHPRT1
UseCRISPR and LentiviralTagsNucleoplasmin NLS and SV40 NLSExpressionMutationPromoterEF1a/hU6Available sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003 sgPIGA guide 1
Plasmid#193610PurposePIGA knockoutDepositorInsertsgPIGA guide 1 (PIGA Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003 sgPIGA guide 2
Plasmid#193611PurposePIGA knockoutDepositorInsertsgPIGA guide 2 (PIGA Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003 sgPIGW guide 1
Plasmid#193612PurposePIGW knockoutDepositorInsertsgPIGW guide 1 (PIGW Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003 sgPIGW guide 2
Plasmid#193613PurposePIGW knockoutDepositorInsertsgPIGW guide 2 (PIGW Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLX317 TFAP4
Plasmid#193616PurposeExpresses TFAP4DepositorInsertTFAP4 (TFAP4 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003 sgNFkBIA guide 1
Plasmid#193595PurposeNFkBIA knockoutDepositorInsertsgNFkBIA guide 1 (NFKBIA Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003 sgNFkBIA guide 2
Plasmid#193596PurposeNFkBIA knockoutDepositorInsertsgNFkBIA guide 2 (NFKBIA Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003 sgSNRPA guide 1
Plasmid#193598PurposeSNRPA knockoutDepositorInsertsgSNRPA guide 1 (SNRPA Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003 sgSNRPA guide 2
Plasmid#193599PurposeSNRPA knockoutDepositorInsertsgSNRPA guide 2 (SNRPA Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003 sgPGD guide 1
Plasmid#193602PurposePGD knockoutDepositorInsertsgPGD guide 1 (PGD Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003 sgPGD guide 2
Plasmid#193603PurposePGD knockoutDepositorInsertsgPGD guide 2 (PGD Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003_sgTFAP4 guide 1
Plasmid#193586PurposeTFAP4 knockoutDepositorInsertsgTFAP4 guide 1 (TFAP4 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003_sgTFAP4 guide 2
Plasmid#193587PurposeTFAP4 knockoutDepositorInsertsgTFAP4 guide 2 (TFAP4 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Gly-tRNA-gRNA scaffold for library 2
Plasmid#192365PurposetRNA sequence provider (for checkpoint library)DepositorInsertGly tRNA-gRNA scaffold
UseFor golden gate assemblyTagsExpressionMutationPromoterAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pro-tRNA-gRNA scaffold for library 2
Plasmid#192366PurposetRNA sequence provider (for checkpoint library)DepositorInsertPro tRNA-gRNA scaffold
UseFor golden gate assemblyTagsExpressionMutationPromoterAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
Gln-tRNA-gRNA scaffold for library 1&2
Plasmid#192367PurposetRNA sequence provider (for checkpoint and immune library)DepositorInsertGln tRNA-gRNA scaffold
UseFor golden gate assemblyTagsExpressionMutationPromoterAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
Gly-tRNA-gRNA scaffold for library 1
Plasmid#192368PurposetRNA sequence provider (for immune library)DepositorInsertGly tRNA-gRNA scaffold
UseFor golden gate assemblyTagsExpressionMutationPromoterAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pro-tRNA-gRNA scaffold for library 1
Plasmid#192369PurposetRNA sequence provider (for immune library)DepositorInsertPro tRNA-gRNA scaffold
UseFor golden gate assemblyTagsExpressionMutationPromoterAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
VAMP8
Plasmid#186689PurposeExpresses VAMP8 in E. ColiDepositorInsertVAMP8
UseTags3xFLAG, GST, TEV, and ThrombinExpressionBacterialMutationPromoterT7Available sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBA1031
Plasmid#189584PurposeEdH4gp0214 deletion HR vector 250bp HR Arms (EdH4 phage editing)DepositorInsertEdH4gp0214 Deletion Locus 250bp Homology Arms
UseSynthetic BiologyTagsExpressionBacterialMutationEncodes for a full deletion of EdH4gp0214PromoterAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBA1030
Plasmid#189582PurposeEdH4gp0004 deletion HR vector 250bp HR Arms (EdH4 phage editing)DepositorInsertEdH4gp0004 Deletion Locus 250bp Homology Arms
UseSynthetic BiologyTagsExpressionBacterialMutationEncodes for a full deletion of EdH4gp0004PromoterAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
Stx3
Plasmid#186685PurposeExpresses Syntaxin 3 in E. ColiDepositorInsertSyntaxin 3
UseTags6 his tagExpressionBacterialMutationPromoterAvailable sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET15 PemrR-gfp biosensor
Plasmid#185793PurposeThe pET15 PemrR-gfp biosensor is transcriptional fusion of GFP with the promoter of the emrRAB operon. This biosensor is responsive to monoaromatics, such as vanillin and syringaldehyde.DepositorInsertpEmrR-gfp
UseTagsEGFPExpressionBacterialMutationPromoterPemrRAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFRTpsiCHECK_SEAP
Plasmid#154461PurposeExpression of SEAP and firefly luciferaseDepositorInsertSEAP
UseTagsExpressionMutationPromoterAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_MTOR
Plasmid#183311PurposeAll-in-One CRISPRko system with a guide RNA that targets MTOR geneDepositorInsertMTOR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_BRAF
Plasmid#183273PurposeAll-in-One CRISPRko system with a guide RNA that targets BRAF geneDepositorInsertBRAF
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1041
Plasmid#169619PurposeTier-2 vector encoding PPGK-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (A1-pA::A2-pA::PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertPPGK-driven expression of secreted Nluc and mTagBFP2
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1040
Plasmid#169618PurposeTier-2 vector encoding PPGK-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (A1-pA::PPGK-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).DepositorInsertPPGK-driven expression of secreted Nluc and mTagBFP2
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_CYP19A1
Plasmid#183280PurposeAll-in-One CRISPRko system with a guide RNA that targets CYP19A1 geneDepositorInsertCYP19A1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only