We narrowed to 10,973 results for: CHL

1. CROPseq-Guide-Puro

   Plasmid

   #86708

   Purpose

   Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.

   Depositor

   Christoph Bock

   Article

   Datlinger et al Nat Methods. 2017 Jan 18. doi: 10.1038/n

   Insert

   EF1a-Puro-WPRE-hU6-gRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-ChrimsonR-tdT

   Plasmid

   #59169

   Purpose

   Expression vector for ChrimsonR-tdTomato

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR-tdTomato
   

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   K176R

   Promoter

   CAG

   Available Since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CaMKIIa-hChR2(E123T/T159C)-EYFP

   Plasmid

   #35511

   Purpose

   AAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.

   Depositor

   Karl Deisseroth

   Article

   Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

   Insert

   hChR2
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E123T and T159C

   Promoter

   CaMKIIa

   Available Since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. MSCV-IRES-NRASG12D-BFP

   Plasmid

   #236202

   Purpose

   Expresses NRASG12D in mammalian cells

   Depositor

   Michael Heuser

   Article

   Mohanty et al Blood Adv. 2025 May 7:bloodadvances.2024

   Insert

   NRAS (NRAS Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   G12D

   Promoter

   MSCV

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-Ef1a-DIO hChR2(E123A)-EYFP

   Plasmid

   #35507

   Purpose

   AAV expression of EF1a-driven, cre-dependent, hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

   Depositor

   Karl Deisseroth

   Article

   Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

   Insert

   hChR2
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E123A

   Promoter

   Ef1a

   Available Since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-luciferase

   Plasmid

   #55764

   Purpose

   luciferase reporter for in vivo bioluminescence imaging

   Depositor

   Snorri Thorgeirsson

   Article

   Lee et al Cancer Res. 2010 Nov 1;70(21):8264-9. do

   Insert

   CMV enhancer/chicken beta actin promoter
   

   Expression

   Mammalian

   Promoter

   CMV enhancer/chicken beta actin promoter

   Available Since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNatA (pACYCduet-naa10-naa15)

   Plasmid

   #72928

   Purpose

   Allows expression of a modified version of the fission yeast NatA complex - chloramphenicol marker

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

   Inserts

   naa10 (naa10 Fission Yeast, Synthetic)
   naa15 (naa15 Fission Yeast)
   

   Expression

   Bacterial

   Mutation

   Codon optomised for E.coli expression

   Promoter

   T7

   Available Since

   Feb. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMpGWB337

   Plasmid

   #68061

   Purpose

   Gateway-based vector for Marchantia polymorpha to express a floxed gene, with an expression cassette for Cre-glucocorticoid receptor fusion protein under the control of heat-inducible promoter

   Depositor

   Takayuki Kohchi

   Article

   Nishihama et al Plant Cell Physiol. 2015 Jul 6. pii: pcv

   Type

   Empty backbone

   Use

   Cre/Lox

   Expression

   Plant

   Promoter

   MpEF1alpha promoter

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XV5

   Plasmid

   #217510

   Purpose

   KD Recombinase dependent conditional smGdP-10XV5 cassette

   Depositor

   S. Lawrence Zipursky

   Article

   Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

   Insert

   KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XV5
   

   Tags

   smGdP-10XV5

   Expression

   Insect

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWPXL-c-Myc

    Plasmid

    #36980

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    c-Myc (Myc Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pTRIP-CMV-GFP-FLAG-cGAS E225A-D227A

    Plasmid

    #86674

    Purpose

    Expresses catalytically-inactive GFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

    Depositor

    Nicolas Manel

    Article

    Raab et al Science. 2016 Apr 15;352(6283):359-62. d

    Insert

    GFP-FLAG-cGAS E225A/D227A
    

    Use

    Lentiviral

    Available Since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET30a-His6-LOXCAT

    Plasmid

    #140084

    Purpose

    Fusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2.

    Depositor

    Vamsi Mootha

    Article

    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

    Insert

    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMT1-24X-NOG(AtUBI10)

    Plasmid

    #202016

    Purpose

    T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the AtUBI10 promoter.

    Depositor

    Michael Smanski

    Article

    Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

    Inserts

    dCas9-24XGP41
    NbGP41P-sfGFP-VP64-GB1
    

    Tags

    GB1 and sfGFP

    Expression

    Plant

    Promoter

    AtUBI10

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. HA-EglN3-pcDNA3

    Plasmid

    #18960

    Depositor

    William Kaelin

    Article

    Lee et al Cancer Cell. 2005 Aug . 8(2):155-67.

    Insert

    Egg laying Nine - 3 (EGLN3 Human)
    

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wt cDNA

    Available Since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. HA-EglN2-pcDNA3

    Plasmid

    #18961

    Depositor

    William Kaelin

    Article

    Lee et al Cancer Cell. 2005 Aug . 8(2):155-67.

    Insert

    Egg laying Nine - 2 (EGLN2 Human)
    

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wt cDNA

    Available Since

    June 19, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTP261

    Plasmid

    #104166

    Purpose

    Bacterial expression plasmid of anti-GFP nanobody with 1x Cysteine for maleimide labeling

    Depositor

    Dirk Görlich

    Article

    Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

    Insert

    Anti-GFP nanobody Enhancer PDB 3K1K (1x Cysteine)
    

    Tags

    14x Histidine tag and NEDD8 from Brachypodium dis…

    Expression

    Bacterial

    Mutation

    1x Cysteine located at bps 493-495 in full sequen…

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. tetO-MEF2C

    Plasmid

    #46031

    Depositor

    John Gearhart

    Article

    Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

    Insert

    MEF2C (MEF2C Human)
    

    Use

    Lentiviral

    Tags

    His and V5

    Expression

    Mammalian

    Promoter

    tetO

    Available Since

    July 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBSFI-myr-akt-T308D/S473D

    Plasmid

    #49192

    Purpose

    cDNA of Akt1 with myristyolation signal and point mutation from T to D at position 308 and S to D at position 473 in cloning vector

    Depositor

    Peter Vogt

    Article

    Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Adaptor plasmid

    Tags

    HA tag and myristolation signal

    Mutation

    T308D; S473D

    Promoter

    T7

    Available Since

    Dec. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. tetO-GATA4

    Plasmid

    #46030

    Depositor

    John Gearhart

    Article

    Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

    Insert

    GATA4 (GATA4 Human)
    

    Use

    Lentiviral

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    R43Q, G50S and N197S

    Promoter

    tetO

    Available Since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-Ef1a-DIO hChR2(E123T/T159C)-mCherry

    Plasmid

    #35510

    Purpose

    AAV expression of Ef1a-driven, cre-dependent, hChR2 variant for ultrafast optogenetic control.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    hChR2
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E123T and T159C

    Promoter

    Ef1a

    Available Since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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