We narrowed to 3,042 results for: GRI;

1. pFastBac Dual wt p110α/p85α

   Plasmid

   #199302

   Purpose

   co-expression of His-tagged p110α wt and Avi-tagged p85α wt proteins

   Depositor

   Peter Vogt

   Article

   Favalli et al Nat Chem. 2021 Jul;13(7):714. doi: 10.10

   Insert

   His-tagged p110α wt and Avi-tagged p85α wt (PIK3CA, PIK3R1 Human)
   

   Use

   Tags

   Avi and His

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. IST1-GFP

   Plasmid

   #186298

   Purpose

   Expresses human IST1 C-terminally fused to GFP

   Depositor

   Geert van den Bogaart

   Article

   Stempels et al J Cell Biol. 2023 Jul 3;222(7):e20220513

   Insert

   IST1 homolog (IST1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   GFP

   Promoter

   CMV

   Available since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKLV2-EF1a-Cas9Bsd-W

   Plasmid

   #68343

   Purpose

   Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the C-terminus

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   EF1a-Cas9 cassette, WPRE
   

   Use

   CRISPR and Lentiviral

   Tags

   Cas9 fused with Flag-tag and a NLS at the N termi…

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP

   Plasmid

   #67991

   Purpose

   CRISPR gRNA expression vector with an improved l scaffold and puro/BFP markers without WPRE

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette with an improved scaffold, PGKpuro2ABFP cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGP-pcDNA3.1 Puro-CAG-Voltron2

   Plasmid

   #172909

   Purpose

   Mammalian expression of voltage sensor

   Depositor

   GENIE Project

   Article

   Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

   Insert

   Voltron2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A122D

   Promoter

   CAG

   Available since

   Nov. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W

   Plasmid

   #67979

   Purpose

   Cas9 activity reporter (control) with BFP and GFP.

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKBFP2AGFP cassette, WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Deleted BbsI site within WPRE

   Promoter

   human U6 promoter, mouse PGK promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKLV2-U6gRNA5(gBFP)-PGKmCherry2ABFP-W

   Plasmid

   #67986

   Purpose

   Cas9 activity reporter with mCherry and BFP

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Inserts

   U6gRNA cassette, PGKmCherryABFP cassette, WPRE
   Guide RNA targeting modified BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Deleted BbsI site within WPRE, modified BFP

   Promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.0-Magneto2.0-p2A-mCherry

   Plasmid

   #74308

   Purpose

   Bicistronic expression of Magneto2.0 and mCherry in mammalian cells

   Depositor

   Ali Guler

   Article

   Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

   Insert

   Magneto2.0 (FTL, FTH1, Trpv4 Human, Rat)
   

   Use

   Tags

   Flag and p2A-mCherry

   Expression

   Mammalian

   Mutation

   TRPV4: delta 760–871

   Promoter

   CMV

   Available since

   May 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGGS-ACE2-his expression plasmid

   Plasmid

   #158089

   Purpose

   Expresses ACE2 in mammalian cells

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   ACE2 (ACE2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   See depositor comments below

   Promoter

   Available since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. plenti-FLAG-CAMK2D

    Plasmid

    #221699

    Purpose

    Lentiviral expression of FLAG-tagged human CAMK2D in mammalian cells

    Depositor

    Derrick Sek Tong Ong

    Article

    Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

    Insert

    CAMK2D (CAMK2D Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNeg-Ma-barnase-TAG3-TAG45

    Plasmid

    #197572

    Purpose

    Negative selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses 2xTAG-codon interrupted barnase gene and M. alvus Pyl-tRNA(6). p15a origin of replication.

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    Barnase - 2xTAG
    M. alvus Pyl-tRNA (6)
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    3TAG and 45TAG

    Promoter

    araC and lpp

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPos-Ma-CmR-TAG111

    Plasmid

    #197571

    Purpose

    Positive selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses TAG-codon interrupted chloramphenicol resistance cassette and M. alvus Pyl-tRNA(6). p15a ori

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    Chloramphenicol resistance gene - 111TAG
    T7 polymerase -TAG
    GFPuv
    M. alvus Pyl-tRNA(6)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    111TAG and 8TAG/112TAG

    Promoter

    AraC, T7, cat promoter (constitutive), and lpp (c…

    Available since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKLV2-U6gRNA5(Empty)-PGKmCherry2ABFP-W

    Plasmid

    #67985

    Purpose

    Cas9 activity reporter (control) with mCherry and BFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKmCherryABFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-pBG-mCRE98-NLS-EGFP-WPRE

    Plasmid

    #239977

    Purpose

    adeno-associated virus (AAV) encoding enhanced green fluorescent protein (eGFP) under motor neuron-selective control of minimal beta globin promoter (pBG) and CRE98

    Depositor

    Sinisa Hrvatin

    Article

    Nagy et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

    Insert

    EGFP (eGFP Synthetic)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    pBG + mCRE98

    Available since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. plenti-FLAG-CAMK2D-ED

    Plasmid

    #221700

    Purpose

    Lentiviral expression of FLAG-tagged human CAMK2D (K43R/D136N) in mammalian cells

    Depositor

    Derrick Sek Tong Ong

    Article

    Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

    Insert

    CAMK2D (CAMK2D Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    K43R, D136N

    Promoter

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLVET-HA-K-RasG12V-IRES-GFP

    Plasmid

    #107140

    Purpose

    A bicistronic lentiviral mammalian expression vector for HA-KRasG12V(4B) and GFP

    Depositor

    Aki Manninen

    Article

    Zhang et al Oncogene. 2017 Oct 12;36(41):5681-5694.

    Insert

    HA-tagged Kras4B(G12V) and GFP (KRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hEF-1alpha

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. loxP-G418-loxP-3xALFA-Halo-Rab11 HR

    Plasmid

    #229676

    Purpose

    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with Halotag and 3x ALFA tag. Contains a G418 resistance cassette for selection of edited cells

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

    Insert

    genomic sequence Rab11 locus (RAB11A Human)
    

    Use

    CRISPR

    Tags

    Halo, 3xALFA

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pscALPSpuro-RsACE2

    Plasmid

    #159665

    Purpose

    Expresses Rhinolophus sinicus ACE2 in mammalian cells

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    rhinolophus sinicus ACE2 (ACE2 rhinolophus sinicus)
    

    Use

    Lentiviral

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. anti-Desmin_D7-pBIOCAM5

    Plasmid

    #39346

    Depositor

    John McCafferty

    Article

    Schofield et al Genome Biol. 2007;8(11):R254.

    Insert

    scFv-Fc-fusion specific for Desmin (clone D7) (DES Human)
    

    Use

    Tags

    3xFLAG, 6xHis, and human Fc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET21a(+)-HIV RT-6xHis-RBS-prot

    Plasmid

    #159149

    Purpose

    expresses His-tagged HIV reverse transcriptase and untagged HIV protease

    Depositor

    Andrea Pauli

    Article

    Kellner et al Front Mol Biosci. 2022 Apr 1;9:801309. d

    Inserts

    Human immunodeficiency virus 1 (HIV-1) reverse transcriptase
    Human immunodeficiency virus 1 (HIV-1) protease
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    HIV-1 group M/subtype B – BH10 strain

    Promoter

    Available since

    Sept. 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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