We narrowed to 148,810 results for: ins;

1. pcDNA3.1(+)_Granulin 4-no linker

   Plasmid

   #176925

   Purpose

   express Granulin 4 without linker 5 in mammalian cells

   Depositor

   Thomas Kukar

   Article

   Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

   Insert

   Granulin 4 (GRN Human)
   

   Tags

   Twin-Strep and FLAG tags encoded after Signal Pep…

   Expression

   Mammalian

   Available Since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSCV-FKBP-FES

   Plasmid

   #221307

   Purpose

   Expression of FES (pLxIS) in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

   Insert

   FES (pLxIS) (Fes Mouse)
   

   Use

   Retroviral

   Tags

   FLAG-4xFKBP

   Available Since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1 EWS-myc-HIS

   Plasmid

   #46386

   Purpose

   expresses myc, HIS tagged EWSR1 in mammalian cells

   Depositor

   Heinz Gehring

   Article

   Pahlich et al Proteins. 2008 Sep;72(4):1125-37. doi: 1

   Insert

   EWSR1 (EWSR1 Human)
   

   Tags

   His and myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 29, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pJW2172

   Plasmid

   #163096

   Purpose

   CRISPR repair template: add insertion site specific homology arms by PCR before insertion

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   30aa linker::mNeonGreen (dpi)::3xFLAG::10aa linker
   

   Use

   CRISPR

   Expression

   Worm

   Mutation

   Silent mutations to remove piRNA sites

   Available Since

   Jan. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. TurboID-13x-EV

   Plasmid

   #215086

   Purpose

   Expresses TurboID-13x[linker] in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   TurboID (birA )
   

   Use

   Retroviral

   Tags

   13x [linker]

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-T7-SpCas9-HF1-P2A-EGFP (RTW3505)

   Plasmid

   #139995

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9-HF1(N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9-HF1 with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   HF1=N497A/R661A/Q695A/Q926A

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. YA1532: pAAV_ CAG-FLEX-paQuasAr3

   Plasmid

   #107702

   Purpose

   in vivo voltage imaging

   Depositor

   Adam Cohen

   Article

   Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

   Insert

   paQuasAr3
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAWG-dCas9-VPR

   Plasmid

   #63802

   Purpose

   SP-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; drosophila vector

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

   Insert

   SP-dCas9-VPR
   

   Use

   CRISPR and Synthetic Biology

   Tags

   VPR (VP64-p65-Rta)

   Expression

   Insect

   Promoter

   act5c

   Available Since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pMVA2

   Plasmid

   #119951

   Purpose

   Expresses recombinant MVA pathway in Escherichia coli

   Depositor

   Nigel Scrutton

   Article

   Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

   Inserts

   mvaE
   mvaS
   mvaK1
   mvaK2
   mvaD
   idi
   

   Expression

   Bacterial

   Mutation

   A110G

   Available Since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCX0393

    Plasmid

    #205984

    Purpose

    Ga0116245_10002768 T7/LacO-Cas6 + Cas14-TwinStrep + Cas7 + His14-Cas5::pJ23119-truncated DR-truncated Fn spacer

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Inserts

    Ga0116245_10002768 candidate type VII proteins
    Cas14
    Cas5
    truncated CRISPR array
    

    Tags

    14xHis and TwinStrep-tag

    Expression

    Bacterial

    Promoter

    T7 (proteins), pJ23119 (crRNA)

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. YA1531: pAAV_CAG-FLEX-QuasAr3

    Plasmid

    #107701

    Purpose

    in vivo voltage imaging

    Depositor

    Adam Cohen

    Article

    Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

    Insert

    QuasAr3
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJW2072

    Plasmid

    #154334

    Purpose

    Crispr repair template: add insertion site specific homology arms by PCR before insertion

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::mScarlet-I (dpi)::3xMyc::10aa linker
    

    Use

    CRISPR

    Expression

    Worm

    Mutation

    Silent mutations to remove piRNA sites

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT7-G-LbCas12a crRNA-BsaI cassette (MSP3495)

    Plasmid

    #114068

    Purpose

    T7 promoter crRNA entry vector used for in vitro transcription of LbCas12a guides

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    entry vector for LbCas12a crRNAs
    

    Use

    In vitro transcription

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMS-eGFP-RTP801

    Plasmid

    #65057

    Purpose

    Expression of RTP801 in mammalian cells

    Depositor

    Cristina Malagelada, Lloyd Greene

    Article

    Malagelada et al J Neurosci. 2006 Sep 27;26(39):9996-1000

    Insert

    DDIT4 (Ddit4 Rat)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV-IE

    Available Since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pBabepuro3-p18 A61D

    Plasmid

    #24937

    Depositor

    Lynda Chin

    Article

    Wiedemeyer et al Cancer Cell. 2008 Apr . 13(4):355-64.

    Insert

    p18 (CDKN2C Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    A61D

    Available Since

    June 10, 2010

    Availability

    Academic Institutions and Nonprofits only

16. YA1629: pAAV_CAG-FLEX-paQuasAr3-s

    Plasmid

    #107703

    Purpose

    in vivo voltage imaging

    Depositor

    Adam Cohen

    Article

    Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

    Insert

    paQuasAr3
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. YA1627: pAAV_hSyn-Dio-paQuasAr3-s-P2A-CheRiff-s

    Plasmid

    #107704

    Purpose

    in vivo voltage imaging

    Depositor

    Adam Cohen

    Article

    Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

    Insert

    paQuasAr3-P2A-CheRiff
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. Venus-Bcl2-pEGFP-C1

    Plasmid

    #177417

    Purpose

    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bcl-2.

    Depositor

    David Andrews

    Article

    Aranovich et al Mol Cell. 2012 Mar 30;45(6):754-63. doi:

    Insert

    Bcl-2 (BCL2 Human)
    

    Tags

    Venus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a-FKBP-STING(pLxIS)

    Plasmid

    #221333

    Purpose

    Overexpression of STING CTT in bacteria

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    STING CTT (Sting1 Mouse)
    

    Tags

    6xHis-4xFKBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1(+)_Granulin 2+linker3

    Plasmid

    #176920

    Purpose

    express Granulin 2 with linker 3 in mammalian cells

    Depositor

    Thomas Kukar

    Article

    Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

    Insert

    Granulin 2+linker3 (GRN Human)
    

    Tags

    Twin-Strep and FLAG tags encoded after Signal Pep…

    Expression

    Mammalian

    Available Since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge