We narrowed to 3,452 results for: ttl

1. pEC3006 (pSpy1C-PZn_ffluc)

   Plasmid

   #218515

   Purpose

   replicative E. coli-S. pyogenes shuttle plasmid for zinc-inducible expression of the Firefly luciferase in S. pyogenes

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Insert

   ffluc
   

   Use

   Luciferase

   Expression

   Bacterial

   Mutation

   deletion of Plac_mrfp cassette

   Promoter

   PZn (PczcD)

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. SPDK3860

   Plasmid

   #199245

   Purpose

   Modified Tobacco rattle virus (TRV) RNA2 vector for editing of Nicotiana benthamiana Phytoene desaturase (PDS) gene

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

   Insert

   Modified TRV RNA2 with PEBV promoter and AtIleu-tRNA mobile RNA sequence
   

   Use

   T-dna vector

   Promoter

   pPEBV

   Available Since

   June 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBS4S

   Plasmid

   #55170

   Purpose

   Empty vector, integration at thrC, ampr, specr

   Depositor

   Thorsten Mascher

   Article

   Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

   Type

   Empty backbone

   Use

   Synthetic Biology; Bacillus biobrick box

   Promoter

   none

   Available Since

   Aug. 1, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pNW-sfGFP(SPS6)

   Plasmid

   #133814

   Purpose

   Fluorescent protein sfGFP(SPS6) variant for optimized expression in Bacillus mycoides.

   Depositor

   Oscar Kuipers

   Article

   Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

   Insert

   sfGFP(SPS6)
   

   Use

   E.coli/bacillus spp. shuttle vector

   Expression

   Bacterial

   Promoter

   Ppta

   Available Since

   Nov. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNICKclos1.0

   Plasmid

   #73639

   Purpose

   Genome editing for gene pyrE (CAC-002) in Clostridium acetobutylicum ATCC 824

   Depositor

   Sheng Yang

   Article

   Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

   Inserts

   Cas9 nickase
   sGRNA to pyrE
   

   Use

   E.coli-clostridium shuttle vector

   Mutation

   D10A

   Promoter

   j23119 and ptb

   Available Since

   June 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOCC305

   Plasmid

   #118898

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with 3C and C-terminal HIS6, cleavable with 3C

   Depositor

   PEPC MPI CBG, Marc Bickle

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-TwinStrep-MBP-3C-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
   

   Tags

   HIS6, cleavable with 3C protease and TwinStrep-MB…

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pXD70LacZ6-LacI-Ptac(LacO)-LacZ

   Plasmid

   #191621

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (KanR), IPTG-inducible promoter-lacZ, engineered from pMAL-c2X

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   lacZ
   

   Expression

   Bacterial

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQ166

   Plasmid

   #109328

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS without promoter

   Depositor

   Yiping Qi

   Article

   Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

   Insert

   zCas9
   

   Use

   CRISPR; Gateway compatible zcas9 entry clone

   Tags

   3x FLAG, NLS and NLS

   Expression

   Plant

   Mutation

   Zea mays codon-optimized Cas9

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBSV2G_P0826-BB0323-mCherryBb

   Plasmid

   #118244

   Purpose

   Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes a BB0323-mCherry translational fusion under the control of the bb0826 promoter

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

   Insert

   BB0323-mCherry translational fusion under the control of the bb0826 promoter
   

   Tags

   mCherry

   Expression

   Bacterial

   Available Since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJAK184

    Plasmid

    #167281

    Purpose

    MazF-based mutagenesis vector for C. difficile

    Depositor

    Robert Fagan

    Article

    Fuchs et al Proc Natl Acad Sci U S A. 2021 Jun 22;11

    Type

    Empty backbone

    Use

    E. coli - c. difficile shuttle vector

    Promoter

    Pxyl

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXD70CT5.1-PxylR-CymR, Pveg(2CuO)-LacZ

    Plasmid

    #191628

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1 (tandem CymR binding sites)-LacZ

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    lacZ
    

    Expression

    Bacterial

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pdCASclos

    Plasmid

    #73640

    Purpose

    Transcriptional repression for gene Spo0A (CAC-2011) in Clostridium acetobutylicum ATCC 824

    Depositor

    Sheng Yang

    Article

    Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

    Inserts

    dCas9
    sgRNA to spo0A
    

    Use

    E.coli-clostridium shuttle vector

    Mutation

    D10A, H840A

    Promoter

    Pj23119 and ptb

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBS1ClacZ

    Plasmid

    #55171

    Purpose

    lacZ-reporter vector, integration at amyE, ampr, cmr

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Type

    Empty backbone

    Use

    Synthetic Biology; Bacillus biobrick box

    Promoter

    none

    Available Since

    Aug. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pCast

    Plasmid

    #200169

    Purpose

    E. coli/Yeast shuttle vector carrying CloNAT marker and Spcas9 allowing cloning of gRNA flanked by tRNA promoter and HDV ribozyme

    Depositor

    Yong-Su Jin

    Article

    Kuanyshev et al Biotechnol Bioeng. 2021 Jan;118(1):372-3

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAD-SPS6-Pman

    Plasmid

    #133906

    Purpose

    Fluorescent protein sfGFP(SPS6) variant for optimized expression in Bacillus mycoides; under control of mannose inducible promoter.

    Depositor

    Oscar Kuipers

    Article

    Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

    Insert

    sfGFP(SPS6)
    

    Use

    E.coli/bacillus spp. shuttle vector

    Expression

    Bacterial

    Promoter

    Pman

    Available Since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDGE476

    Plasmid

    #153267

    Purpose

    M2E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAD-mKate(KPS12)

    Plasmid

    #133831

    Purpose

    Fluorescent protein mKate(KPS12) variant for optimized expression in Bacillus mycoides.

    Depositor

    Oscar Kuipers

    Article

    Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

    Insert

    mKate(KPS12)
    

    Use

    E.coli/bacillus spp. shuttle vector

    Expression

    Bacterial

    Promoter

    Pupp

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. attB53-Pac-TRE-mCherry-attB53

    Plasmid

    #183611

    Purpose

    RMCE vector to shuttle puromycin resistance and TRE-mCherry cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).

    Depositor

    Sally Lowell

    Article

    Malaguti et al Development. 2022 May 26. pii: 275525. d

    Insert

    TRE-mCherry
    

    Use

    Rmce

    Promoter

    TRE

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYPQ230-E795L

    Plasmid

    #176890

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing LbCas12a with E795L mutation, without promoter

    Depositor

    Yiping Qi

    Article

    Zhang et al Front Genome Ed. 2022 Jan 31;4:780238. d

    Insert

    LbCas12a-E795L
    

    Use

    CRISPR; Gateway compatible lbcpf1 entry clone

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Plant

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDGE657

    Plasmid

    #153255

    Purpose

    M2E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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