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CMV-FOXA1-T2A-eGFP

Plasmid

#182337

Purpose

Expresses human FOXA1 and eGFP via T2A linker under control of a CMV promoter.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

Forkhead Box A1 (FOXA1 Human)

Use

Tags

T2A-eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-FOXA1-T2A-miRFP670

Plasmid

#182335

Purpose

Expresses human FOXA1 and miRFP670 via T2A linker under control of a CMV promoter.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

Forkhead Box A1 (FOXA1 Human)

Use

Tags

T2A-miRFP670

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pRAR064

Plasmid

#185031

Purpose

400 nt l31 promoter region, scrambled 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

Depositor

Julius Lucks

Article

Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

Insert

L31-sfGFP

Use

Tags

Expression

Bacterial

Mutation

Nucleotides of rpmE 5'UTR scrambled, Only fi…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRAR074-control-sfGFP

Plasmid

#185032

Purpose

J23108 promoter, scrambled 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

Depositor

Julius Lucks

Article

Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

Insert

L31-sfGFP

Use

Tags

Expression

Bacterial

Mutation

Nucleotides of rpmE 5'UTR scrambled, Only fi…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRAR102

Plasmid

#185033

Purpose

400 nt l31 promoter region, l31 5'UTR U54C mutation, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

Depositor

Julius Lucks

Article

Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

Insert

L31-sfGFP

Use

Tags

Expression

Bacterial

Mutation

U54C mutation of rpmE 5'UTR, Only first 90 n…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRAR106

Plasmid

#185036

Purpose

400 nt l31 promoter region, l31 5'UTR G79U mutation, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

Depositor

Julius Lucks

Article

Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

Insert

L31-sfGFP

Use

Tags

Expression

Bacterial

Mutation

G79U mutation of rpmE 5'UTR, Only first 90 n…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRAR116

Plasmid

#185040

Purpose

400 nt l31 promoter region, l31 5'UTR U58C and U70C, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

Depositor

Julius Lucks

Article

Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

Insert

L31-sfGFP

Use

Tags

Expression

Bacterial

Mutation

U58C and U70C of rpmE of 5'UTR, Only first 9…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRAR017-L31-sfGFP

Plasmid

#185024

Purpose

400 nt l31 promoter region, l31 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

Depositor

Julius Lucks

Article

Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

Insert

L31-sfGFP

Use

Tags

Expression

Bacterial

Mutation

Only first 90 nt of rpmE coding present

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSFDuet-1-Cdc45-6His

Plasmid

#211379

Purpose

bacterial expression of human Cdc45 C-terminally fused to 6xHis-Tag

Depositor

Helmut Pospiech

Article

Szambowska et al Nucleic Acids Res. 2014 Feb;42(4):2308-1

Insert

Cdc45 (CDC45 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57-RPLP15UTR-hRluc-A60

Plasmid

#182639

Purpose

Transcription template plasmid for RPLP1 5'UTR followed by Renilla luciferase open reading frame and polyA sequence

Depositor

Stephen Floor

Article

Calviello et al Nucleic Acids Res. 2021 May 21;49(9):533

Insert

Renilla luciferase

Use

Luciferase

Tags

Expression

Mammalian

Mutation

The transcription is driven by a T7 promoter, pai…

Promoter

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP1a-H19A_V

Plasmid

#147799

Purpose

Mammalian Expression of HsDCP1a-H19A

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

Insert

HsDCP1a-H19A (DCP1A Human)

Use

Tags

Expression

Mammalian

Mutation

two silent mutations T237C and T1716G compared to…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP1a-N74G_V

Plasmid

#147800

Purpose

Mammalian Expression of HsDCP1a-N74G

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

Insert

HsDCP1a-N74G (DCP1A Human)

Use

Tags

Expression

Mammalian

Mutation

two silent mutations T237C and T1716G compared to…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP1a-Q18AH19A_V

Plasmid

#147801

Purpose

Mammalian Expression of HsDCP1a-Q18AH19A

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

Insert

HsDCP1a-Q18AH19A (DCP1A Human)

Use

Tags

Expression

Mammalian

Mutation

two silent mutations T237C and T1716G compared to…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-HsDCP1a-R72G_V

Plasmid

#147802

Purpose

Mammalian Expression of HsDCP1a-R72G

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

Insert

HsDCP1a-R72G (DCP1A Human)

Use

Tags

Expression

Mammalian

Mutation

two silent mutations T237C and T1716G compared to…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP1a-R72GN74G_V

Plasmid

#147803

Purpose

Mammalian Expression of HsDCP1a-R72GN74G

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

Insert

HsDCP1a-R72GN74G (DCP1A Human)

Use

Tags

Expression

Mammalian

Mutation

two silent mutations T237C and T1716G compared to…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-lambdaN-HA-HsDCP2_1-244_V

Plasmid

#147790

Purpose

Mammalian Expression of HsDCP2_1-244

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

Insert

HsDCP2_1-244 (DCP2 Human)

Use

Tags

Expression

Mammalian

Mutation

one silent mutation A429G compared to the sequenc…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-HsTNRC6A_1209-1709-CIM1-2mut_S

Plasmid

#147521

Purpose

Bacterial Expression of HsTNRC6A_1209-1709-CIM1-2mut

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6A_1209-1709-CIM1-2mut (TNRC6A Human)

Use

Tags

Expression

Bacterial

Mutation

one non silent mutation K1578E compared to the se…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-6P-1-HsTNRC6A_1209-1709-CIM1-2mut-F1359A_S

Plasmid

#147522

Purpose

Bacterial Expression of HsTNRC6A_1209-1709-CIM1-2mut-F1359A

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6A_1209-1709-CIM1-2mut-F1359A (TNRC6A Human)

Use

Tags

Expression

Bacterial

Mutation

one non silent mutation K1578E compared to the se…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-6P-1-HsTNRC6A_1209-1709-M2WA-CIM1-2mut_S

Plasmid

#147527

Purpose

Bacterial Expression of HsTNRC6A_1209-1709-M2W1-CIM1-2mut

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6A_1209-1709-M2W1-CIM1-2mut (TNRC6A Human)

Use

Tags

Expression

Bacterial

Mutation

one non silent mutation K1578E compared to the se…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-6P-1-HsTNRC6A_1209-1709-M2WA-F1359A_S

Plasmid

#147528

Purpose

Bacterial Expression of HsTNRC6A_1209-1709-M2W1-F1359A

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6A_1209-1709-M2W1-F1359A (TNRC6A Human)

Use

Tags

Expression

Bacterial

Mutation

one non silent mutation K1578E compared to the se…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge