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Plasmid#66137PurposeExpresses the T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3DepositorInsertCYP119 (cyp119 )
UseTagsSulfolobus solfataricus PCNA3ExpressionBacterialMutationT214VPromoterT7 promoterAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA3 T180C
Plasmid#66181PurposeExpresses the T180C mutant of Sulfolobus solfataricus PCNA3DepositorInsertDNA polymerase sliding clamp 3
UseTagsHis6 tagExpressionBacterialMutationT180CPromoterT7 promoterAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA3 R112C
Plasmid#66180PurposeExpresses the R112C mutant of Sulfolobus solfataricus PCNA3DepositorInsertDNA polymerase sliding clamp 3
UseTagsHis6 tagExpressionBacterialMutationR112CPromoterT7 promoterAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA2 E105C
Plasmid#66176PurposeExpresses the E105C mutant of Sulfolobus solfataricus PCNA2DepositorInsertDNA polymerase sliding clamp 2
UseTagsHis6 tagExpressionBacterialMutationE105CPromoterT7 promoterAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJexpress414_OmpA171_WTnoGirdles
Plasmid#62653PurposeWild-type OmpA with aromatic girdle residues replaced by leucineDepositorInsertOmpA_noGirdles
UseSynthetic BiologyTags6His and FlAsHExpressionBacterialMutationAromatic girdle residues mutated to Leu (W7, W15,…PromoterT7Available sinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
AI-CN491
Plasmid#62594PurposeTALEN array receiving vector with half-site NN RVDDepositorTypeEmpty backboneUseTALEN; T7Tags3× FLAG and FOKI WTExpressionMammalianMutationPromoterCMVAvailable sinceMarch 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
AI-CN443
Plasmid#62593PurposeTALEN array receiving vector with half-site NG RVDDepositorTypeEmpty backboneUseTALEN; T7Tags3× FLAG and FOKI WTExpressionMammalianMutationPromoterCMVAvailable sinceMarch 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
AI-CN442
Plasmid#62591PurposeTALEN array receiving vector with half-site HD RVDDepositorTypeEmpty backboneUseTALEN; T7Tags3× FLAG and FOKI WTExpressionMammalianMutationPromoterCMVAvailable sinceMarch 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
AI-CN441
Plasmid#62590PurposeTALEN array receiving vector with half-site NI RVDDepositorTypeEmpty backboneUseTALEN; T7Tags3× FLAG and FOKI WTExpressionMammalianMutationPromoterCMVAvailable sinceMarch 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAD06 K. lactis telomerase RNA hairpin
Plasmid#61895PurposePlasmid for run-off in vitro transcription of hairpin from the Kluyveromyces lactis telomerase RNA (nt 229-273)DepositorInsertTelomerase RNA hairpin (nt 229-273)
UseTagsExpressionMutationPromoterT7Available sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28-p53 (Guinea Pig 1-88)
Plasmid#62059PurposeExpression of Guinea Pig p53 (residues 1-88) in e. coliDepositorInsertp53
UseTagsHisExpressionBacterialMutationcontains TAD residues 1-88PromoterT7Available sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC19 gene 60 thru gap
Plasmid#49160PurposeTemplate for generating gene 60 mRNA truncated two codons after coding gapDepositorInsertBacteriophage T4 gene 60
UseTagsExpressionMutationgene 60 mRNA truncated two codons after coding ga…PromoterT7Available sinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC19 gene 60 to gap + Cys
Plasmid#49159PurposeTemplate for generating gene 60 mRNA, sequence 5' of coding gap + CysDepositorInsertBacteriophage T4 gene 600
UseTagsExpressionMutationCoding gap and downstream sequence have been dele…PromoterT7Available sinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
SpDJ-1 pET15b
Plasmid#60689PurposeRecombinant protein expression of DJ-1DepositorInsertDJ-1 (SPAC22E12.03c Fission Yeast)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28b(+)-JHP933
Plasmid#59302DepositorInsertjhp0933
UseTagsHis tagExpressionBacterialMutationaa1-232PromoterT7Available sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSC-hXRCC1-K176R-IntE2
Plasmid#52277PurposeBacterial expression of human XRCC1-K176R C-terminally fused to GyrA intein, SUMO-E2 and GSTDepositorInsertXRCC1 (XRCC1 Human)
UseTagsGyrA-intein-Ubc9-GSTExpressionBacterialMutationK176R, SUMO acceptor site mutantPromoterT7Available sinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSC-hXRCC1-K176R-PreE2
Plasmid#52279PurposeBacterial expression of human XRCC1-K176R C-terminally fused to SUMO-E2 and GSTDepositorInsertXRCC1 (XRCC1 Human)
UseTagsUbc9-GSTExpressionBacterialMutationK176R, SUMO acceptor site mutantPromoterT7Available sinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET20b- hTPI Ile170Thr
Plasmid#50725Purposeoverexpression of the human TPI1 Ile170Thr allele in E.coliDepositorInsertTriosephosphate isomerase 1 (TPI1 Human)
UseTags6xHIS tagExpressionBacterialMutationchanged Isoleucine 170 to ThreoninePromoterT7Available sinceFeb. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-UNC60B
Plasmid#48740PurposeBacterial expression of UNC-60B (a C. elegans ADF/cofilin)DepositorInsertC. elegans UNC-60B cDNA (unc-60 Nematode)
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-UNC60A
Plasmid#48739PurposeBacterial expression of UNC-60A (a C. elegans ADF/cofilin)DepositorInsertC. elegans UNC-60A cDNA (unc-60 Nematode)
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
Danio rerio cryaa
Plasmid#46166PurposeRecombinant protein productionDepositorInsertAlpha A-crystallin (cryaa Zebrafish)
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
Cyprinodon variegatus cryaa
Plasmid#47664PurposeRecombinant protein productionDepositorInsertAlpha A-crystallin (cryaa Zebrafish)
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
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TAL3476
Plasmid#47195DepositorInsertZebrafishCommunity-klf8-left (si:ch211-208m1.2 Zebrafish)
UseT7Tags3X Flag and WT FOKIExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
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TAL3487
Plasmid#47206DepositorInsertZebrafishCommunity-matrixmetalloproteinase14a(membrane-inserted)-right
UseT7Tags3X Flag and WT FOKIExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
TAL3486
Plasmid#47205DepositorInsertZebrafishCommunity-matrixmetalloproteinase14a(membrane-inserted)-left
UseT7Tags3X Flag and WT FOKIExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
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TAL3485
Plasmid#47204DepositorInsertZebrafishCommunity-matrixmetallopeptidase15(membrane-inserted)-right
UseT7Tags3X Flag and WT FOKIExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
TAL3484
Plasmid#47203DepositorInsertZebrafishCommunity-matrixmetallopeptidase15(membrane-inserted)-left
UseT7Tags3X Flag and WT FOKIExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
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