We narrowed to 8,836 results for: FIE

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pT3TS-spCas9 ABE-Umax-ex1-rest1

Plasmid

#222140

Purpose

Adenine base editor with modified TadA variant for extended window and restricted A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Article

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

Modified TadA-nSpCas9

Use

CRISPR; Zebrafish expression

Tags

Bipartite NLS

Promoter

T3

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-sgCTRL-1

Plasmid

#83934

Purpose

Lentiviral vector expressing a control sgRNA. NOTE: This plasmid does NOT contain Cas9.

Depositor

David Sabatini

Article

Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

Insert

sgCTRL-1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1 IntS8 AA 1-308

Plasmid

#196905

Purpose

Expresses His-tagged Drosophila IntS8 amino acids 1-308

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

Integrator subunit 8 (IntS8 Fly)

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-PGK- dCas9-SunTag-P2A-HygR

Plasmid

#193137

Purpose

Expresses dCas9 fused to the SunTag scaffold

Depositor

Brad Rosenberg

Article

Danziger et al PLoS Pathog. 2022 Apr 14;18(4):e1010464.

Insert

dCas9-SunTag-T2A-HygR

Use

Lentiviral

Promoter

PGK

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pv6_CBX7_2xChromo

Plasmid

#179396

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_2xChromo

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

CBX7_2xChromo

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

G113V in flexible linker

Promoter

CAGGS

Available Since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pcs2+spCas9 ABE-Umax-nanos

Plasmid

#222142

Purpose

Adenine base editor with modified TadA variant for germline A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Article

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

Modified TadA-nSpCas9 with nanos UTR

Use

CRISPR; Zebrafish expression

Tags

Bipartite NLS

Promoter

SP6

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-SP110C

Plasmid

#65393

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

SP110C

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
GFP-SP110A

Plasmid

#65392

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

SP110A

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
pBabe_puro_DEST_Flag_PLD1

Plasmid

#45268

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

PLD1 (PLD1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

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aav-U6-broccoli-hsp68-mCherry-MCS-pA

Plasmid

#195411

Purpose

AAV9 based enhancer activity assay

Depositor

William Pu

Article

Akerberg et al Nat Commun. 2019 Oct 28;10(1):4907. doi:

Type

Empty backbone

Use

AAV

Available Since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-ERK1

Plasmid

#14747

Depositor

Rony Seger

Article

Yung et al J Biol Chem. 2001 Sep 21. 276(38):35280-

Insert

ERK1 (MAPK3 Human)

Tags

EGFP

Expression

Mammalian

Available Since

June 12, 2007

Availability

Academic Institutions and Nonprofits only

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HA-TEAD1

Plasmid

#229411

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

TEAD1 (TEAD1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

EFS

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMP71-super-IL-2

Plasmid

#174599

Purpose

Expresses extracellular domain of human IL-2 (aa 21-153) (mutant H9 containing the mutations L80F / R81D / L85V / I 86V / I92F) developed by the Garcia Lab

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

extracellular domain of huIL-2 (aa 21-153) (mutant H9 containing the mutations L80F / R81D / L85V / I 86V / I92F)

Expression

Mammalian

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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THBS1-bio-His

Plasmid

#53417

Purpose

Expresses full-length Thrombospondin-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

THBS1 (THBS1 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE hSPC-eGFP-W

Plasmid

#36450

Depositor

Darrell Kotton

Article

Longmire et al Cell Stem Cell. 2012 Apr 6;10(4):398-411

Inserts

human SP-C promoter
eGFP (eGFP )

Use

Lentiviral

Promoter

N/A and human SP-C promoter

Available Since

May 11, 2012

Availability

Academic Institutions and Nonprofits only

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pLV_hU6-sgRNA_hUbC-dSaCas9-KRAB-T2A-PuroR

Plasmid

#162334

Purpose

Lentiviral expression of dSaCas9-KRAB and a sgRNA with puromycin resistance

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

dSaCas9-KRAB

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

hUbC

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-MT GLI2 delta N

Plasmid

#17649

Depositor

Erich Roessler

Article

Roessler et al Hum Mol Genet. 2005 Aug 1. 14(15):2181-8

Insert

GLI2 (GLI2 Human)

Tags

6x Myc

Expression

Mammalian

Mutation

Missing 328 N-terminal amino acids

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pv6_MBD1_MBD

Plasmid

#179404

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1_MBD

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

MBD1_MBD

Tags

AviTag and EGFP

Expression

Mammalian

Promoter

CAGGS

Available Since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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PEAR1-bio-His

Plasmid

#51860

Purpose

Expresses full-length Platelet endothelial aggregation receptor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

PEAR1 (PEAR1 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFPc3 N-RAS

Plasmid

#236450

Purpose

transient overexpression of N-RAS in mammalian cells

Depositor

Michele Pagano

Article

Kuchay et al Nat Struct Mol Biol. 2019 Jul;26(7):628-

Insert

N-RAS (NRAS Human)

Expression

Mammalian

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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