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We narrowed to 4,337 results for: U6 gRNA

Showing: 2721 - 2740 of 4337 results

  1. pLCHKO_SFRS7_exon_deletion_2

    Plasmid
    #155070
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    155070-plasmid-map-sequence-id-308588

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  2. pLCHKO_MDM4_exon_deletion_1

    Plasmid
    #155073
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    155073-plasmid-map-sequence-id-308586

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  3. pLCHKO_MDM4_exon_deletion_2

    Plasmid
    #155074
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    155074-plasmid-map-sequence-id-308585

    Enlarge

  4. pLCHKO_MDM4_exon_deletion_4

    Plasmid
    #155076
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    155076-plasmid-map-sequence-id-308577

    Enlarge

  5. pLCHKO_HPRT1_exon3_1_As

    Plasmid
    #155057
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and AsCas12a nucleases (CHyMErA system)
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of HPRT1 exon 3
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155057-plasmid-map-sequence-id-308520

    Enlarge

  6. pLCHKO_SFRS7_exon_deletion_4

    Plasmid
    #155072
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155072-plasmid-map-sequence-id-308512

    Enlarge

  7. pLCHKO_PDPR_exon_deletion_3

    Plasmid
    #155067
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155067-plasmid-map-sequence-id-308446

    Enlarge

  8. pLCHKO_PDPR_exon_deletion_4

    Plasmid
    #155068
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155068-plasmid-map-sequence-id-308444

    Enlarge

  9. pLCHKO_HPRT1_exon3_2_As

    Plasmid
    #155058
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and AsCas12a nucleases (CHyMErA system)
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of HPRT1 exon 3
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155058-plasmid-map-sequence-id-308441

    Enlarge

  10. pLCHKO_HPRT1_exon2_2_As

    Plasmid
    #155056
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and AsCas12a nucleases (CHyMErA system)
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of HPRT1 exon 2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155056-plasmid-map-sequence-id-308440

    Enlarge

  11. pLCHKO_HPRT1_exon2_1_As

    Plasmid
    #155055
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and AsCas12a nucleases (CHyMErA system)
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of HPRT1 exon 2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155055-plasmid-map-sequence-id-308438

    Enlarge

  12. pLCHKO_HPRT1_exon2_2_Lb

    Plasmid
    #155052
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of HPRT1 exon 2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155052-plasmid-map-sequence-id-308434

    Enlarge

  13. B-SpCas9

    Plasmid
    #126760
    Purpose
    Expression plasmid for human codon-optimized Blackjack-SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of WT SpCas9.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-SpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    amino acids 1005-1013 replaced with two glycine
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126760-plasmid-map-sequence-id-261519

    Enlarge

  14. pCAT009

    Plasmid
    #171635
    Purpose
    Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting B2M and CAG promoter expressing mTagBFP2
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-B2M
    mTagBFP2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and U6
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171635-plasmid-map-sequence-id-336490

    Enlarge

  15. pCAT005

    Plasmid
    #171632
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting B2M and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-B2M
    mNeon
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-a and U6
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171632-plasmid-map-sequence-id-336487

    Enlarge

  16. pJRH623

    Plasmid
    #171633
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a non-targeting sgRNA and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-non-targeting
    mNeon
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-a and U6
    Available since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171633-plasmid-map-sequence-id-336492

    Enlarge

  17. pCAT007

    Plasmid
    #171634
    Purpose
    Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and CAG promoter expressing mTagBFP2
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-TRAC
    mTagBFP2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and U6
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171634-plasmid-map-sequence-id-336493

    Enlarge

  18. pJRH056

    Plasmid
    #171637
    Purpose
    Plasmid containing a U6 promoter expressing a spyCas9 tdTom sgRNA298 and CAG promoter expressing mTagBFP2
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-tdTomato
    mTagBFP2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and U6
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171637-plasmid-map-sequence-id-354439

    Enlarge

  19. pCAT024

    Plasmid
    #171636
    Purpose
    Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting BFP and CAG promoter expressing mTagBFP2
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-BFP
    mTagBFP2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and U6
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    171636-plasmid-map-sequence-id-337539

    Enlarge

  20. B-SpCas9-HF1

    Plasmid
    #126762
    Purpose
    Expression plasmid for human codon-opt. increased fidelity Blackjack-SpCas9-HF1 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than SpCas9HF1.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-SpCas9-HF1
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A; R661A; Q695A; Q926A; amino acids 1005-1013…
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126762-plasmid-map-sequence-id-410965

    Enlarge

Showing: 2721 - 2740 of 4337 results