We narrowed to 17,127 results for: URE

1. pAAV-CaMKIIa-C1V1 (t/t)-TS-EYFP

   Plasmid

   #35499

   Purpose

   AAV expression of CaMKII-driven chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.

   Depositor

   Karl Deisseroth

   Article

   Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

   Has Service

   AAV9

   Insert

   ChR1-VChR1 Chimera
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E122T and E162T

   Promoter

   CaMKIIa

   Available since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-Ef1a-DIO C1V1 (t/t)-TS-EYFP

   Plasmid

   #35497

   Purpose

   AAV expression of Ef1a-driven, cre-dependent, chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.

   Depositor

   Karl Deisseroth

   Article

   Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

   Has Service

   AAV9

   Insert

   ChR1-VChR1 Chimera
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E122T and E162T

   Promoter

   Ef1a

   Available since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMB952:PB-CAG-PuroR-nGFP-FLEx-SaCas9-P2A-mCherry-WPRE

   Plasmid

   #168107

   Purpose

   PiggyBac transposon vector constitutively driving PuroR-eGFP and conditionally expressing S.aureus Cas9 and mCherry

   Depositor

   Erich Jarvis

   Article

   Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

   Insert

   Cas9-P2A-mCherry
   

   Use

   CRISPR and Cre/Lox

   Tags

   HA

   Expression

   Mutation

   Promoter

   CAG

   Available since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-Ef1a-DIO hChR2(C128S/D156A)-mCherry

   Plasmid

   #35504

   Purpose

   AAV expression of Ef1a-driven, cre-dependent, stabilized step function opsin (SSFO) for optogenetic activation.

   Depositor

   Karl Deisseroth

   Article

   Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

   Insert

   hChR2(C128S/D156A)-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   C128S and D156A

   Promoter

   Ef1a

   Available since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. SHMT2-RES

   Plasmid

   #72880

   Purpose

   overexpression

   Depositor

   David Sabatini

   Article

   Kim et al Nature. 2015 Apr 16;520(7547):363-7. doi

   Insert

   SHMT2 (SHMT2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   silent mutations in site corresponding to shSHMT2…

   Promoter

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pAAV-CaMKIIa-hChR2(C128S/D156A)-mCherry

   Plasmid

   #35502

   Purpose

   AAV expression of CaMKII-driven stabilized step function opsin (SSFO) for optogenetic activation.

   Depositor

   Karl Deisseroth

   Article

   Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

   Insert

   hChR2(C128S/D156A)-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   C128S and D156A

   Promoter

   CaMKIIa

   Available since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV hSyn FLEx-FRT Kir2.1-2A-GFP

   Plasmid

   #161577

   Purpose

   Expression of Kir2.1-2A-GFP in a Flp-dependent fashion

   Depositor

   Kevin Beier, Robert Malenka

   Article

   Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

   Insert

   Kir2.1-2A-GFP (Kcnj2 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   Available since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBRY-nuclear mCherry-IRES-PURO

   Plasmid

   #52409

   Purpose

   Constitutively expressed mammalian expression vector encoding nuclear mCherry fluorescent reporter. Puromycin resistance.

   Depositor

   Jacob Hanna

   Article

   Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

   Insert

   nuclear mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. MRP1-CD4d3+4-bio

   Plasmid

   #73120

   Purpose

   EXPRESs plasmid for human erythrocyte surface proteins encoding MRP1 with rat CD4d3+4-bio

   Depositor

   Gavin Wright

   Article

   Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

   Insert

   MRP1 (ABCC1 Human)
   

   Use

   Tags

   biotinylation peptide and ratCD4d3+4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-TET-mSox2

    Plasmid

    #20908

    Purpose

    piggyBac vector with tet inducible mSox2

    Depositor

    Andras Nagy

    Article

    Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

    Insert

    Sox2 (Sox2 Mouse)
    

    Use

    piggybac

    Tags

    IRES-bGeo

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. Flag-Jun4A-Myc

    Plasmid

    #47444

    Purpose

    mammalian expression of mutant c-Jun (four N-terminal residues phosphorylated by JNK are mutated into Alanines)

    Depositor

    Axel Behrens

    Article

    Aguilera et al Nature. 2011 Jan 13;469(7329):231-5. doi

    Insert

    Jun 4A mutant (Jun Mouse)
    

    Use

    Tags

    Flag and myc

    Expression

    Mammalian

    Mutation

    four N-terminal residues phosphorylated by JNK, s…

    Promoter

    CMV

    Available since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTub-alpha1-EB3-NeonGreen-T2A-iLifeact-mCherry

    Plasmid

    #175261

    Purpose

    labelling of microtubule plus end and F-actin structures

    Depositor

    Frank Bradke, Sebastián Dupraz

    Article

    Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

    Insert

    EB3-NeonGreen-T2A-iLifeact-mCherry (MAPRE3 Human)
    

    Use

    Tags

    mCherry (on iLifeact) and mNeonGreen (on EB3)

    Expression

    Mammalian

    Mutation

    None (wt)

    Promoter

    pTub-alpha1

    Available since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB-TET-mKLF4

    Plasmid

    #20906

    Purpose

    piggyBac vector with tet inducible mKLF4

    Depositor

    Andras Nagy

    Article

    Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

    Insert

    KLF4 (Klf4 Mouse)
    

    Use

    piggybac

    Tags

    IRES-bGeo

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-N1_MeCP2(R306C)

    Plasmid

    #110188

    Purpose

    Expression of mouse MeCP2 (R306C) in mammalian cells

    Depositor

    Adrian Bird

    Article

    Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

    Insert

    MeCP2_e2 FL [R306C] (mouse cDNA) (Mecp2 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Arg 306 Cys

    Promoter

    CMV

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. bio-CD4d3+4-Kell blood group

    Plasmid

    #73089

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding Kell blood group with bio-rat CD4d3+4

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    Kell blood group (KEL Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCold I-Hero11

    Plasmid

    #187928

    Purpose

    Bacterial expression of heat-resistant obscure (Hero) protein Hero11

    Depositor

    Yukihide Tomari

    Article

    Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

    Insert

    Hero11 (C19orf53 Human)
    

    Use

    Tags

    Flag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUASTLOTattB_eGFP::Dpp

    Plasmid

    #163702

    Purpose

    GFP-tagged version of Dpp (Decapentaplegic) under control of UAS and LexO enhancers

    Depositor

    Markus Affolter

    Article

    Harmansa et al Nature. 2015 Nov 19;527(7578):317-22. do

    Insert

    decapentaplegic (dpp Fly)
    

    Use

    Cre/Lox

    Tags

    Tagged with eGFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Duffy-CD4d3+4-bio

    Plasmid

    #73122

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding Duffy with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    Duffy (ACKR1 Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PGL4-4X Mut Renilla

    Plasmid

    #70040

    Purpose

    Control plasmid for testing 5'UTR folding elements

    Depositor

    Hans-Guido Wendel

    Article

    Wolfe et al Nature. 2014 Sep 4;513(7516):65-70. doi:

    Insert

    Random sequence matched for GC-content
    

    Use

    Luciferase

    Tags

    renilla luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pEGFP-N1_MeCP2(deltaNC)

    Plasmid

    #110190

    Purpose

    Expression of mouse MeCP2 (deltaNC) in mammalian cells

    Depositor

    Adrian Bird

    Article

    Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

    Insert

    MeCP2_e2 delta NC (mouse cDNA) - EGFP (Mecp2 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    delta N and C termini

    Promoter

    CMV

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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