We narrowed to 2,365 results for: DAP

1. qTAG-C-Solo-mStayGold-PXN

   Plasmid

   #227316

   Purpose

   Donor template for mStayGold insertion into the C-terminus of the PXN locus. For focal adhesion visualization. To be co-transfected with sgRNA plasmid px330-PITCh-PXN (Addgene #227315)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   PXN Homology Arms flanking a mStayGold Tag (PXN Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. qTAG-C-Solo-mScarlet-H2BC11

   Plasmid

   #207756

   Purpose

   Donor template for mScarlet insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a mScarlet Tag (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 16, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pmiRGLO Dual-Luciferase-PIK3R1-3'UTR

   Plasmid

   #182252

   Purpose

   Human PIK3R1 3` UTR region containing 1x binding site for miR-150 cloned downstream of luciferase reporter gene

   Depositor

   Silvia Monticelli

   Article

   Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

   Insert

   3' UTR region of PIK3R1 gene
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCas4_1_2_VA

   Plasmid

   #186438

   Purpose

   Encodes Cas4, Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   Cas4, Cas1 and Cas2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-AMPKsub-YPet-NES

   Plasmid

   #84635

   Purpose

   Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   AMPKsub-YPet-NES
   

   Use

   Tags

   6xHis, Nuclear export signal (NES), T7 tag (gene …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRS315e_pGal-dCas9-SH3-pGal-PmCDA1-SHL

   Plasmid

   #79614

   Purpose

   Expresses dCas9-SH3 and PmCDA1-SHL in yeast cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Inserts

   SpCas9
   PmCDA1
   

   Use

   Tags

   SH3 domain and SHL

   Expression

   Yeast

   Mutation

   D10A and H840A for nuclease deficient Cas9

   Promoter

   pGal1 and pGal10

   Available Since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-4T-1-β4 hinge-ear

   Plasmid

   #196692

   Purpose

   Expresses GST-AP-4 β4 hinge-ear (residues 534-739) in bacteria

   Depositor

   Juan Bonifacino

   Article

   Mattera et al J Biol Chem. 2015 Dec 25;290(52):30736-4

   Insert

   AP-4 β4 hinge-ear (534-739) (AP4B1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-mCherry-LKB1(KD)

   Plasmid

   #84640

   Purpose

   mCherry-tagged, LKB1(K78I); kinase-dead mutant

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   mCherry-LBK1(K78I) (STK11 Human, Synthetic)
   

   Use

   Tags

   6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …

   Expression

   Mammalian

   Mutation

   Lysine 78 mutated to Isoleucine; kinase dead

   Promoter

   CMV

   Available Since

   May 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. Intersectin I SH3 A domain (human)

   Plasmid

   #47413

   Depositor

   Peter McPherson

   Article

   Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

   Insert

   Intersectin SH3 domain A (ITSN1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   only SH3 domain A

   Promoter

   tac

   Available Since

   July 29, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pGEX4T1-GST-LC3B

    Plasmid

    #216836

    Purpose

    For expression in E. coli of GST-LC3B (Gly exposed) (Internal ref. SMC1463)

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    LC3B (Gly exposed) (MAP1LC3B Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    TAC promoter

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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