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pQE-WT-CGG-R12-KIR

Plasmid

#63626

Purpose

CGG-R12-KIR RNAP mutant

Depositor

Andrew Ellington

Article

Meyer et al ACS Synth Biol. 2014 Oct 7.

Insert

K1F IKR RNAP

Mutation

Q744K, L747V, N748H, L749I, R756E, L757M, H772R

Promoter

T5/Lac

Available Since

July 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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NK644

Plasmid

#109096

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

PTSG_00306 geranyl-geranylation signal

Use

Choanoflagellate expression

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pQE-WT-N4-R5-RNRV

Plasmid

#63630

Purpose

N4-R5-RNRV RNAP mutant

Depositor

Andrew Ellington

Article

Meyer et al ACS Synth Biol. 2014 Oct 7.

Insert

N4-R5-RNRV RNAP

Mutation

L747I, N748D, L749C, M750V, F751I, Q754T, F755R, …

Promoter

T5/Lac

Available Since

April 15, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pICXC3H

Plasmid

#49022

Purpose

FX cloning Pichia pastoris expression vector with AOX1 promoter and C-terminal 3C protease cleavage site and 10x His tag

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Yeast

Promoter

AOX1

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR4-V5-2 (w234-1)

Plasmid

#17426

Purpose

Gateway entry vector for N-term V5 tag, frame B

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Entry vector

Tags

V5

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pYEXC3GH

Plasmid

#49027

Purpose

FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tag

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Yeast

Promoter

GAL1

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pJSM101

Plasmid

#48791

Purpose

NHEJ reporter plasmid for 5' and 3' cohesive ends

Depositor

Michael Glickman

Article

Zhu et al Biochemistry. 2012 Dec 21;51(51):10147-5

Insert

split lacZ

Expression

Bacterial

Available Since

Dec. 17, 2013

Availability

Academic Institutions and Nonprofits only

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gS gag LV

Plasmid

#12327

Depositor

Inder Verma

Article

Kootstra et al Proc Natl Acad Sci U S A. 2003 Feb 4. 10

Insert

gag/pol

Use

Lentiviral

Expression

Mammalian

Mutation

The CypA binding region of the WT gag was replace…

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only
pENTR1a-jAspSnFR3-mRuby3

Plasmid

#203491

Purpose

jAspSnFR3-mRuby3 in Gateway cloning entry vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

jAspSnFR3-mRuby3

Use

Gateway entry vector

Tags

His-tag and mRuby3

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pAcUW51-gE2

Plasmid

#11621

Depositor

Pamela Bjorkman

Article

Sprague et al J Biol Chem. 2004 Apr 2. 279(14):14184-9

Insert

HSV-1 glycoprotein E

Tags

His

Expression

Insect

Mutation

deleted amino acids 391-550

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only