3,636 results

1. pMAL c2x Tral FL-15A

   Plasmid

   #113011

   Purpose

   For bacterial expression of MBP fusion of full-length Drosophila Tral with phosphomutations (15A)

   Depositor

   Terry Orr-Weaver

   Article

   Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

   Insert

   full length tral (tral Fly)
   

   Expression

   Bacterial

   Mutation

   15 PNG phosphorylation sites mutated to A

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. UASp-deltaN cycB

   Plasmid

   #112967

   Purpose

   P element vector for transposon insertion of nondegradable Cyclin B into Drosophila genome

   Depositor

   Terry Orr-Weaver

   Article

   Lee et al Genetics. 2001 Aug;158(4):1545-56.

   Insert

   cycB (CycB Fly)
   

   Use

   P insertion vector

   Mutation

   N-term deletion of first 46 amino acids, includes…

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMAL c2x Tral C

   Plasmid

   #113008

   Purpose

   For bacterial expression of MBP fusion of C terminal region of Drosophila Tral

   Depositor

   Terry Orr-Weaver

   Article

   Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

   Insert

   C terminus of tral (tral Fly)
   

   Expression

   Bacterial

   Mutation

   amino acids 356-652

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. Yes-10kb

   Plasmid

   #112996

   Purpose

   P element vector for transposon insertion of the 10Kb Drosophila Amplicon in Follicle Cells (DAFC-62D) into Drosophila genome

   Depositor

   Terry Orr-Weaver

   Article

   Xie et al Proc Natl Acad Sci U S A. 2008 Jul 15;10

   Insert

   10Kb DAFC-62D amplicon
   

   Use

   P insertion vector

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGAL4DBD-SuUR f.l

   Plasmid

   #112995

   Purpose

   P element vector for transposon insertion of full-length Drosophila SuUR into Drosophila genome

   Depositor

   Terry Orr-Weaver

   Article

   Sher et al Genome Res. 2012 Jan;22(1):64-75. doi: 1

   Insert

   SuUR (SuUR Fly)
   

   Use

   P insertion vector

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMAL c2x Tral C 15A

   Plasmid

   #113007

   Purpose

   For bacterial expression of MBP fusion of C terminal region of Drosophila Tral phosphomutant (15A)

   Depositor

   Terry Orr-Weaver

   Article

   Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

   Insert

   C terminus of tral (tral Fly)
   

   Expression

   Bacterial

   Mutation

   PNG phosphorylation sites mutated to A

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEX6P-1 gnu

   Plasmid

   #112999

   Purpose

   For protein expression and purification of full-length Drosophila gnu

   Depositor

   Terry Orr-Weaver

   Article

   Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

   Insert

   full length gnu (gnu Fly)
   

   Expression

   Bacterial

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. E2F1 in pGEX4t

   Plasmid

   #112976

   Purpose

   For protein expression and purification of Drosophila E2F1

   Depositor

   Terry Orr-Weaver

   Insert

   E2F1 (E2f1 Fly)
   

   Expression

   Bacterial

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. mr-GST N-term fusion

   Plasmid

   #112983

   Purpose

   For protein expression and purification of N-terminus of Drosophila mr (APC2)

   Depositor

   Terry Orr-Weaver

   Insert

   mr (APC2) (mr Fly)
   

   Expression

   Bacterial

   Mutation

   N-term only

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFastBac Dual PNG172-STII PLU-HIS

    Plasmid

    #112992

    Purpose

    Baculovirus expression of Drosophila png172-Flag and plu-His

    Depositor

    Terry Orr-Weaver

    Inserts

    png172 (png Fly)
    plu (plu Fly)
    

    Tags

    Flag and His

    Expression

    Insect

    Mutation

    png172 mutation

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFastBac Dual PNG-STII PLU-HIS

    Plasmid

    #112993

    Purpose

    Baculovirus expression of Drosophila png-Flag and plu-His

    Depositor

    Terry Orr-Weaver

    Inserts

    png (png Fly)
    plu (plu Fly)
    

    Tags

    Flag and His

    Expression

    Insect

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMAL c2x Tral FL-15D

    Plasmid

    #113010

    Purpose

    For bacterial expression of MBP fusion of full-length Drosophila Tral with Phos-mimic mutations

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    full length tral (tral Fly)
    

    Expression

    Bacterial

    Mutation

    15 PNG phosphorylation sites mutated to D

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mr-GST full length

    Plasmid

    #112982

    Purpose

    For protein expression and purification of full-length Drosophila mr (APC2)

    Depositor

    Terry Orr-Weaver

    Insert

    mr (APC2) (mr Fly)
    

    Expression

    Bacterial

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

14. pUAST-attB-Flag-dTSC2 S1338A

    Plasmid

    #111808

    Purpose

    Insect Expression of dTSC2

    Depositor

    Jun Hee Lee

    Article

    Kim et al Int J Mol Sci. 2015 Mar 27;16(4):7015-26

    Insert

    dTSC2 (TSC2 Fly)
    

    Tags

    3xFlag

    Expression

    Insect

    Mutation

    S1338A

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. Hy_pMT dati MCS Exon

    Plasmid

    #110119

    Purpose

    Expression plasmid for expressing circular RNAs of a desired sequence in Drosophila using the dati flanking introns to drive backsplicing

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    dati (dati Fly)
    

    Expression

    Insect

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. Hy_pMT dati Exons 1-3

    Plasmid

    #110113

    Purpose

    Drosophila dati minigene plasmid that expresses both a linear RNA and a circular RNA

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    dati (dati Fly)
    

    Expression

    Insect

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX-4T-1 dATG1 (1188)

    Plasmid

    #66918

    Purpose

    bacterial expression of dATG1

    Depositor

    Michael Karin, Jun Hee Lee

    Article

    Kim et al Autophagy. 2013 Aug;9(8):1201-13. doi: 1

    Insert

    ATG1
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMCh-623

    Plasmid

    #92266

    Purpose

    Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 6W6A with six mutated W-motifs in its N-terminal domain in D. melanogaster cells

    Depositor

    Marina Chekulaeva, Witold Filipowicz

    Article

    Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

    Insert

    dGW182 6W6A (gw Fly)
    

    Tags

    NHA

    Expression

    Insect

    Mutation

    The following point mutations were introduced W23…

    Promoter

    Ac5

    Available Since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMCh-695

    Plasmid

    #92263

    Purpose

    Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 with mutated PAM2 motif in D. melanogaster cells

    Depositor

    Marina Chekulaeva, Witold Filipowicz

    Article

    Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

    Insert

    dGW182 PAM2 mut (gw Fly)
    

    Tags

    NHA

    Expression

    Insect

    Mutation

    The following point mutations were introduced: EF…

    Promoter

    Ac5

    Available Since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMCh-61

    Plasmid

    #92262

    Purpose

    Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 in D. melanogaster cells

    Depositor

    Marina Chekulaeva, Roy Parker

    Article

    Chekulaeva et al RNA. 2009 May;15(5):794-803. doi: 10.126

    Insert

    dGW182 (gw Fly)
    

    Tags

    NHA

    Expression

    Insect

    Promoter

    Ac5

    Available Since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge