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pVP076

Plasmid

#222028

Purpose

Empty spectinomycin resistant GreenGate destination vector based on pGGP-AG. Domesticated of Esp3I sites in the backbone and chloramphenicol resistance gene.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All Esp3i sites domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pSuccessor

Plasmid

#228391

Purpose

intended to be co-transformed along with pDawn-Ag43 to generate successor strain (SS-GFP) for multipattern biofilm lithography

Depositor

Ingmar Riedel-Kruse

Article

Jin et al Nat Commun. 2024 Nov 1;15(1):9443. doi:

Insert

pConst23100-sfGFP__pLambda-DHFR

Use

Synthetic Biology

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2-hcar1-4-mKate2

Plasmid

#226712

Purpose

overexpression red fluorescent hcar1-4 protein in zebrafish larvae

Depositor

Philipp Niethammer

Article

Ma et al Cell Rep. 2023 Jan 31;42(1):111974. doi:

Insert

hcar1-4

Use

Unspecified; Zebrafish expression

Tags

mKate2

Promoter

SP6

Available Since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pGEARBOCS-v0- Gfap-TagIN-mCherry

Plasmid

#196490

Purpose

To tag endogenous mouse GFAP with mCherry

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Inserts

mCherry
gRNA

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGEARBOCS-v0-Gfap-GeneTRAP

Plasmid

#196492

Purpose

To KO and genetrap mouse GFAP simultaneously

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Inserts

mCherry-CAAX
gRNA

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGEARBOCS-v0-Vamp2-GeneTRAP

Plasmid

#196493

Purpose

To KO and genetrap mouse VAMP2 simultaneously

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Inserts

mCherry-CAAX
gRNA

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_ZF1_mNeonGreen

Plasmid

#213840

Purpose

Facilitates genomic integration of the reporter with upstream binding sites for ZF-1

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_ZF2_mNeonGreen

Plasmid

#213841

Purpose

Facilitates genomic integration of the reporter with upstream binding sites for ZF-2

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNoRBS

Plasmid

#218167

Purpose

pET31b plasmid with short insert lacking ribosome binding site to increase 2',3'-cNMP levels

Depositor

Emily Weinert

Article

Chauhan et al ACS Cent Sci. 2022 Nov 23;8(11):1518-152

Insert

Short insert

Expression

Bacterial

Promoter

T7

Available Since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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pLBD16-YFP-GW

Plasmid

#209369

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis LBD16 promoter driving expression of N-terminal YFP fusion protein.

Depositor

Lucia Strader

Article

Michniewicz et al BMC Res Notes. 2015 Mar 3;8:63. doi: 10.

Type

Empty backbone

Expression

Plant

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pADH1-YFP-GW

Plasmid

#209364

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis ADH1 promoter driving expression of N-terminal YFP fusion protein.

Depositor

Lucia Strader

Article

Michniewicz et al BMC Res Notes. 2015 Mar 3;8:63. doi: 10.

Type

Empty backbone

Expression

Plant

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCOBL-YFP-GW

Plasmid

#209368

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis COBL promoter driving expression of N-terminal YFP fusion protein.

Depositor

Lucia Strader

Article

Michniewicz et al BMC Res Notes. 2015 Mar 3;8:63. doi: 10.

Type

Empty backbone

Expression

Plant

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCAB1-YFP-GW

Plasmid

#209363

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis CAB1 promoter driving expression of N-terminal YFP fusion protein.

Depositor

Lucia Strader

Article

Michniewicz et al BMC Res Notes. 2015 Mar 3;8:63. doi: 10.

Type

Empty backbone

Expression

Plant

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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mTurquoise2-Scarlet-I

Plasmid

#193558

Purpose

Expresses mTurquoise2-mScarlet-I in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

mTurquoise2-mScarlet-I

Expression

Mammalian

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV-neo C-HAonly HPV16 E7 E80A/D81A

Plasmid

#191541

Purpose

Protein expression of HPV16 E7 E80A/D81A

Depositor

Elizabeth White

Article

Nouel et al mBio. 2022 Nov 8:e0203322. doi: 10.1128/

Insert

HPV16 E7

Use

Retroviral

Tags

HA

Mutation

E80A, D81A

Available Since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK001_P2Ab

Plasmid

#183084

Purpose

2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use in bi-cistronic and as the first 2A peptide in tri-cistronic construct assembly

Depositor

Zhen Wang

Article

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

P2A

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19_BAS1509

Plasmid

#82354

Purpose

T7 in vitro transcription template for Bas mRNA

Depositor

Nils Walter

Article

Chatterjee et al Proc Natl Acad Sci U S A. 2021 Apr 20;11

Insert

BAS1509 hypothetical protein

Use

Unspecified

Promoter

T7

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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SZ1-GST-mCherry-RGG

Plasmid

#176646

Purpose

SynZip1 linked surfactant protein that localizes to RGG condensate interface

Depositor

Daniel Hammer

Article

Guan et al Biochemistry. 2021 Oct 14. doi: 10.1021/

Insert

SZ1-GST-mCherry-RGG

Expression

Bacterial

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pKP1627

Plasmid

#167648

Purpose

Express FepA G54C, delta12-16 (TonB box) . Please note that mutation numbers are not based on start codon of insert.

Depositor

Kathleen Postle

Article

Postle et al bioRxiv 2021.12.08.471779

Insert

fepA G54C, delta12-16 (TonB box)

Expression

Bacterial

Mutation

fepA with G54C, delta12-16 (TonB box) mutations (…

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pKP1193

Plasmid

#167614

Purpose

Express TonB H20N

Depositor

Kathleen Postle

Article

Swayne et al J Bacteriol. 2011 Aug;193(15):3693-701.

Insert

tonB H20N

Expression

Bacterial

Mutation

tonB with H20N mutation

Available Since

July 9, 2021

Availability

Academic Institutions and Nonprofits only

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pKP1626

Plasmid

#167646

Purpose

Express FepA D34C, delta12-16 (TonB box) . Please note that mutation numbers are not based on start codon of insert.

Depositor

Kathleen Postle

Article

Postle et al bioRxiv 2021.12.08.471779

Insert

fepA D34C, delta12-16 (TonB box)

Expression

Bacterial

Mutation

fepA with D34C, delta12-16 (TonB box) mutations. …

Available Since

July 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-V5-SPR

Plasmid

#127236

Purpose

Eukaryotic expression of SPR

Depositor

Kai Johnsson

Article

Haruki et al PLoS One. 2012;7(5):e37598. doi: 10.1371

Insert

SPR (SPR Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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pHMGWA-Cas1

Plasmid

#122285

Purpose

Cas1 cloned in pHMGWA

Depositor

Jennifer Doudna

Article

Nunez et al Nat Struct Mol Biol. 2014 Jun;21(6):528-

Insert

Eco Cas1

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pSB58 (Prp16)

Plasmid

#111282

Purpose

Prp16 in pSE358(Trp)

Depositor

Christine Guthrie

Article

Burgess et al Cell. 1993 Jul 2;73(7):1377-91.

Insert

PRP16

Expression

Yeast

Available Since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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pAP574-1

Plasmid

#99477

Purpose

3XFlag::Topaz::Myc

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::Topaz::Myc

Expression

Bacterial

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAP996-1

Plasmid

#99499

Purpose

3XFlag::meGFP::linker::TEV

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::meGFP::linker::TEV

Expression

Bacterial

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pAP835-1

Plasmid

#99495

Purpose

TEV::meGFP::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::meGFP::3XFlag

Expression

Bacterial

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pAP686-1

Plasmid

#99491

Purpose

TEV::eGFP::ER::V5::linker::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::eGFP::RE::V5::linker::3XFlag

Expression

Bacterial

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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recoverin1a

Plasmid

#83811

Purpose

express zebrafish recoverin1a

Depositor

Stephan Neuhauss

Article

Zang et al Open Biol. 2015 Aug;5(8). pii: 150086. d

Insert

recoverin1a (rcvrna Zebrafish)

Expression

Bacterial

Mutation

*(see below)

Available Since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) TRIM30B

Plasmid

#87082

Purpose

Murine homologues of human Trim5

Depositor

Jeremy Luban

Article

Lascano et al J Virol. 2015 Oct 14;90(1):308-16. doi:

Insert

TRIM30B (Trim30b Mouse)

Expression

Mammalian

Available Since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

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