We narrowed to 7,988 results for: 104

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pDest17_Gcn4 E2K2

Plasmid

#231868

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 E2K2

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 E2K2

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S104K, S117E, S136E, S144K

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pBi2FC-N-MPK6-P2A:mCherry

Plasmid

#216137

Purpose

Controls for Bicistronic BiFC

Depositor

Imran Siddiqi

Article

Sivakumar et al Physiol Mol Biol Plants. 2024 Jul;30(7):

Insert

MPK6 (MPK6 Mustard Weed)

Expression

Plant

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pBi2FC-N-MPK12-P2A:mCherry

Plasmid

#216139

Purpose

Controls for Bicistronic BiFC

Depositor

Imran Siddiqi

Article

Sivakumar et al Physiol Mol Biol Plants. 2024 Jul;30(7):

Insert

MPK12 (MPK12 Mustard Weed)

Expression

Plant

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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BS-ZJLEU-PTEF1-GFP

Plasmid

#207018

Purpose

Over-expression of GFP under TEF1 promoter, use auxotrophic marker LEU2(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

GFP

Expression

Yeast

Promoter

pTEF1

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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PTPI1-Dsred-URA3

Plasmid

#207017

Purpose

Expresses DsRed-Express 2 as a marker of cytosol, uses auxotrophic marker URA3(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

DsRed-Express 2

Expression

Yeast

Promoter

pTPI1

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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BS-TRP1-PGAL-GFP-Cps1

Plasmid

#207030

Purpose

Expression of GFP-Cps1 under GAL promoter.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Cps1

Tags

GFP

Expression

Yeast

Promoter

GAL1

Available Since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mRAB14-HA

Plasmid

#214165

Purpose

Transient expression of RAB14

Depositor

Nan Yan

Article

Tu et al Nat Commun. 2022 Nov 15;13(1):6977. doi:

Insert

RAB14 (Rab14 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 5, 2024

Availability

Academic Institutions and Nonprofits only

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PCUP1-4V5-Pho8-Met

Plasmid

#207029

Purpose

Expression of 4V5-Pho8 under CPU1 promoter.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Pho8

Tags

4xV5

Expression

Yeast

Promoter

pCUP1

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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BS-Trp-PCUP1-4myc-Pho8

Plasmid

#207028

Purpose

Expression of 4Myc-Pho8 under CPU1 promoter.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Pho8

Tags

4xMyc

Expression

Yeast

Promoter

pCUP1

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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PCUP1-Vac8-GFP-Ura

Plasmid

#207027

Purpose

Expression of Vac8-GFP under CPU1 promoter.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Vac8

Tags

GFP

Expression

Yeast

Promoter

pCUP1

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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PCUP1-GFP-Vma13-Ura

Plasmid

#207026

Purpose

Expression of GFP-Vma13 under CPU1 promoter.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Vma13 ORF

Tags

GFP

Expression

Yeast

Promoter

pCUP1

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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P1K-3HA-Atg8∆GR-MET15

Plasmid

#207033

Purpose

Expression of 3HA-Atg8∆GR. This variant cannot be conjugated to PE.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Tags

3xHA

Expression

Yeast

Mutation

∆GR

Promoter

pATG8

Available Since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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PCUP1-mRuby3-Pho8-406

Plasmid

#207043

Purpose

Expression of mRuby3-Pho8 under CPU1 promoter.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

PHO8

Tags

mRuby3

Expression

Yeast

Promoter

pCUP1

Available Since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pLCS100

Plasmid

#209354

Purpose

Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter expression of N-terminal YFP fusion protein.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pUBQ10-HALO-GW

Plasmid

#209351

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal HaloTag fusion protein.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLCS103

Plasmid

#209356

Purpose

Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal SNAP-tag fusion protein.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pJRA106

Plasmid

#209339

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal moxMaple fusion protein.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-6xHis-TrxF

Plasmid

#207454

Purpose

E. coli expression of Mesembryanthemum crystallinum Thioredoxin F with thrombin-cleavable N-terminal 6xHis tag

Depositor

Joseph Jez, Sarah Stainbrook

Article

Stainbrook et al Biosci Rep. 2024 Oct 30;44(10):BSR202403

Insert

Thioredoxin F

Tags

6xHis

Expression

Bacterial

Mutation

Codon optimized for E coli expression

Promoter

T7

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pET-28b(+)/ADK C77S

Plasmid

#201409

Purpose

Overexpresses Escherichia coli adenylate kinase C77S

Depositor

Gary Pielak

Article

Brom et al Biomol NMR Assign. 2023 Aug 26. doi: 10.

Insert

Escherichia coli adenylate kinase C77S (adk Escherichia coli)

Expression

Bacterial

Mutation

changed cysteine 77 to serine

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCI pHluorin Syt8

Plasmid

#184509

Purpose

Mammalian expression of Synaptotagmin 8 with pH-sensitive fluorescent protein

Depositor

Edwin Chapman

Article

Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

Insert

Synaptotagmin 8 (Syt8 Mouse)

Tags

Super Ecliptic pHluorin

Expression

Mammalian

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pT-GFP-rG1

Plasmid

#188967

Purpose

IPTG inducible GFP with sgRNA

Depositor

Seung-Goo Lee

Article

Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

Inserts

GFP
sgRNA: agtggaaaacaatgcgaccgactagt

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptrc

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT-GFP-rG2

Plasmid

#188968

Purpose

IPTG inducible GFP with sgRNA

Depositor

Seung-Goo Lee

Article

Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

Inserts

GFP
sgRNA: agtccatgtaatcagcgtctactagt

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptrc

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGP8GF-ZmAFB2/3 b2

Plasmid

#188425

Purpose

Zea Mays Nuclear Auxin Signaling Receptor AFB2/3 b2

Depositor

Britney Moss

Article

Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

Insert

Auxin Signaling Receptor AFB2/3 b2

Expression

Yeast

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGP8GF-ZmAFB2/3 a

Plasmid

#188423

Purpose

Zea Mays Nuclear Auxin Signaling Receptor AFB2/3 A

Depositor

Britney Moss

Article

Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

Insert

Auxin Signaling Receptor AFB2/3 a

Expression

Yeast

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGP4G-REL2N91-ccdB

Plasmid

#188444

Purpose

Zea Mays Nuclear Auxin Signaling CoRepressor REL2N91 and pDEST

Depositor

Britney Moss

Article

Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

Insert

REL2N91/ccdB

Expression

Yeast

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-ZmIAA8ΔPB1

Plasmid

#188432

Purpose

Zea Mays Nuclear Auxin Signaling Repressor IAA8

Depositor

Britney Moss

Article

Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

Insert

Auxin Signaling Repressor IAA8deltaPB1

Use

Cloning

Mutation

deleted PB1 domain

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
ppwd1.473.646.W601A

Plasmid

#137660

Purpose

Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

Depositor

Tara Davis, Melissa Jurica

Article

Adams et al Biochem J. 2015 Jul 15;469(2):223-33. do

Insert

ppwd1 (PPWD1 Human)

Tags

(His)6-thrombin

Expression

Bacterial

Mutation

473-646-W601A

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
GP826

Plasmid

#164551

Purpose

Expresses Smo-FLAG K444, 448R

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened K444, 448R (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Mutation

Two of the three Lysines in loop three of Smoothe…

Promoter

GgCryD1

Available Since

Feb. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
GP825

Plasmid

#164550

Purpose

Expresses Smo-FLAG K434, 448R

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened K434, 448R (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Mutation

Two of the three Lysines in loop three of Smoothe…

Promoter

GgCryD1

Available Since

Feb. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
GP824

Plasmid

#164549

Purpose

Expresses Smo-FLAG K434, 444R

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened K434, 444R (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Mutation

Two of the three Lysines in loop three of Smoothe…

Promoter

GgCryD1

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
PD30

Plasmid

#164543

Purpose

Expresses Drd1-Ub

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Dopamine receptor D1 (Drd1 Mouse)

Use

Lentiviral

Tags

FLAG-Ub

Mutation

WT

Promoter

CMV

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
GP807

Plasmid

#164535

Purpose

Expresses Smo noK-FLAG-Ub noK

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened noK (Smo Mouse)

Use

Lentiviral

Tags

FLAG_Ub noK

Mutation

All 21 cytoplasmic Lysines of Smoothened and all…

Promoter

CMV

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
MYMH 539

Plasmid

#138078

Purpose

Control vector with no degradation tag to compare against various degradation tag variants

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23104-mCherry::sbfI- pSEVA-331Bb

Expression

Bacterial

Available Since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MYMH 553

Plasmid

#138083

Purpose

Degradation tag variant at C-terminal of mCherry

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23104-mCherry-NYADAS-pSEVA-331Bb

Expression

Bacterial

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MYMH 552

Plasmid

#138082

Purpose

Degradation tag variant at C-terminal of mCherry

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23104-mCherry-DAS- pSEVA-331Bb

Expression

Bacterial

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MYMH 551

Plasmid

#138081

Purpose

Degradation tag variant at C-terminal of mCherry

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23104-mCherry-AAV- pSEVA-331Bb

Expression

Bacterial

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MYMH 550

Plasmid

#138080

Purpose

Degradation tag variant at C-terminal of mCherry

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23104-mCherry-LVA- pSEVA-331Bb

Expression

Bacterial

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MYMH 549

Plasmid

#138079

Purpose

Degradation tag variant at C-terminal of mCherry

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23104-mCherry-LAA- pSEVA-331Bb

Expression

Bacterial

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MYMH 473

Plasmid

#138077

Purpose

Synthetic Terminator L3S1P47 inserted between mCherry and GFP

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23104-mCherry-L3S1P47-GFP-pSEVA-331Bb

Expression

Bacterial

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MYMH 472

Plasmid

#138076

Purpose

Synthetic Terminator L3S2P44 inserted between mCherry and GFP

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23104-mCherry-L3S2P44-GFP-pSEVA-331Bb

Expression

Bacterial

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MYMH 471

Plasmid

#138075

Purpose

Synthetic Terminator L3S2P24 inserted between mCherry and GFP

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23104-mCherry-L3S2P24-GFP-pSEVA-331Bb

Expression

Bacterial

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MYMH 470

Plasmid

#138074

Purpose

Synthetic Terminator L3S3P21 inserted between mCherry and GFP

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23104-mCherry-L3S3P21-GFP-pSEVA-331Bb

Expression

Bacterial

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MYMH 469

Plasmid

#138073

Purpose

Synthetic Terminator L3S2P21 inserted between mCherry and GFP

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23104-mCherry-L3S2P21-GFP-pSEVA-331Bb

Expression

Bacterial

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MYMH 468

Plasmid

#138072

Purpose

Natural Terminator BB0010 inserted between mCherry and GFP

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23104-mCherry-BB0010-GFP-pSEVA-331Bb

Expression

Bacterial

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MYMH 467

Plasmid

#138071

Purpose

Natural Terminator ECK120051401 inserted between mCherry and GFP

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23104-mCherry-51401-GFP-pSEVA-331Bb

Expression

Bacterial

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MYMH 466

Plasmid

#138070

Purpose

Natural Terminator ECK120010799 inserted between mCherry and GFP

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23104-mCherry-10799-GFP-pSEVA-331Bb

Expression

Bacterial

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MYMH 465

Plasmid

#138069

Purpose

Natural Terminator ECK120010818 inserted between mCherry and GFP

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23104-mCherry-10818-GFP-pSEVA-331Bb

Expression

Bacterial

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
INPP5A / type I IP3 5-phosphatase from dog thyroid

Plasmid

#131767

Purpose

Expression in bacteria or COS-7 cells

Depositor

Christophe Erneux

Article

Verjans et al Biochem J. 1994 May 15;300 ( Pt 1):85-90

Insert

INPP5A

Expression

Bacterial

Available Since

Nov. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pC0.178

Plasmid

#119645

Purpose

Level 0 Part. Insertion site

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

7942 NS3 Down Flank

Use

Synthetic Biology

Mutation

4 nucleotide insertion between nt 65 and 66 in NS…

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pC0.165

Plasmid

#119634

Purpose

Level 0 Part. Insertion site

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

6803 NS1 Up Flank

Use

Synthetic Biology

Mutation

1 nucleotide insertion between nt 21 and 22 in NS…

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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