We narrowed to 22,342 results for: Sele

1. pHS1928

   Plasmid

   #205967

   Purpose

   JAGBLH010000220 Cas8-HNH operon (E. coli codon optimized) with His-tagged Cas8 in pET45b(+)

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   Cas8-HNH all proteins, with His-tagged Cas8
   

   Use

   Tags

   14xHis on Cas8

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-CAG-DDdCas9VPPH-T2A-GFP-IRES-Neo

   Plasmid

   #102888

   Purpose

   PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   DDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   CAG

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. AICSDP-55:DMD-mEGFP

   Plasmid

   #159744

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human DMD via Cas9-excisable CAGGS-mCherry selection cassette

   Depositor

   Allen Institute for Cell Science

   Insert

   DMD Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (DMD Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21-Drp1

   Plasmid

   #72927

   Purpose

   Bacterial expression of mouse Drp1 isoform b (699amino acids) with C-terminal His-tag and PreScission protease cleavage site

   Depositor

   David Chan

   Article

   Liu et al Mol Biol Cell. 2015 Dec 1;26(24):4466-77

   Insert

   Drp1 (Dnm1l Mouse)
   

   Use

   Tags

   6xHis-tag and PreScission Protease site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA_B2AR-i-pep

   Plasmid

   #47436

   Purpose

   FRET sensor for beta2-adrenergic receptor

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7

   Insert

   beta2-adrenergic receptor (ADRB2 Human)
   

   Use

   Tags

   10 nm ER/K helix, HA, i-pep, mCerulean, and mCitr…

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV

   Available since

   Sept. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pSIN-Ldha

   Plasmid

   #175939

   Purpose

   knockout mouse Ldha

   Depositor

   Dan Littman

   Article

   Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

   Insert

   Ldha gRNA (Ldha Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. AID-mTagBFP2-loxP_myo2_neoR-loxP

   Plasmid

   #194055

   Purpose

   Dual-selection cassette plasmid for knocking-in AID-mTagBFP2 into the C. elegans genome

   Depositor

   Kota Mizumoto

   Article

   Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

   Insert

   IAA17 (AXR3 Mustard Weed)
   

   Use

   Tags

   codon optimized mTagBFP2

   Expression

   Worm

   Mutation

   Promoter

   None

   Available since

   Jan. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pblic-neo TRX1

   Plasmid

   #110919

   Purpose

   To overexpress Thioredoxin-1

   Depositor

   Priyamvada Rai

   Article

   Samaranayake et al Nat Commun. 2017 Oct 31;8(1):1204. doi:

   Insert

   TRX1 (TXN Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   unknown

   Available since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. aMHC-Puro Rex-Blast

   Plasmid

   #21231

   Depositor

   Mark Mercola

   Article

   Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

   Insert

   alpha myosin heavy chain promoter
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR221_SLC39A8

    Plasmid

    #132177

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Polesel et al Biometals. 2022 Dec 1. doi: 10.1007/s105

    Insert

    SLC39A8 (SLC39A8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 25, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pDONR221_SLC39A10

    Plasmid

    #132275

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Polesel et al Biometals. 2022 Dec 1. doi: 10.1007/s105

    Insert

    SLC39A10 (SLC39A10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 14, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. Agarabi CHO Light Chain in pJ201

    Plasmid

    #111566

    Purpose

    Cloning vector containing CDS for the murine light chain of the model chimeric mouse-human IgG1 expressed by the Agarabi CHO cell line

    Depositor

    Cyrus Agarabi

    Article

    Velugula-Yellela et al Biotechnol Prog. 2018 Jan;34(1):262-270.

    Insert

    Murine light chain
    

    Use

    Synthetic Biology; Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. ATP1A1_T804N_hPGK1_EBFP_Donor

    Plasmid

    #187455

    Purpose

    HDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the EBFP cassette to ATP1A1 intron 17.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1-T804N EBFP donor (ATP1A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK1

    Available since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEBTet-SNAP-Centrobin

    Plasmid

    #136829

    Purpose

    Mammalian expression of the centrosomal protein Centrombin N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-Centrobin (CNTROB Synthetic, Human)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. mTiam1-mcherry-sspB in pcDNA3.1

    Plasmid

    #85221

    Purpose

    Role of Rac selective GEF domain from Tiam1 in immune cell migration

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.

    Inserts

    mTiam1 (1030-1406) (Tiam1 Mouse)
    mcherry
    sspB
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUCmini-iCAP-PHP.B3

    Plasmid

    #103004

    Purpose

    non-standard AAV2 rep-AAV-PHP.B3 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

    Insert

    Synthetic construct isolate AAV-PHP.B3 VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUCmini-iCAP-PHP.B2

    Plasmid

    #103003

    Purpose

    non-standard AAV2 rep-AAV-PHP.B2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

    Insert

    Synthetic construct isolate AAV-PHP.B2 VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available since

    Dec. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pACE-K2P10.1-StrepTagII

    Plasmid

    #191475

    Purpose

    Expresses K2P10.1 with C-terminal StrepTagII

    Depositor

    Arthur Laganowsky

    Article

    Zhu et al J Am Chem Soc. 2022 Apr 27;144(16):7048-

    Insert

    K2P10.1 (KCNK10, TREK-2 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    N144Q (uniprot P57789-1)

    Promoter

    polH

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRSFDuet-1 IntS6 AA 1035-1284

    Plasmid

    #196904

    Purpose

    Expresses His-tagged Drosophila IntS6 amino acids 1035-1284

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    Integrator subunit 6 (IntS6 Fly)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHAGE-FLAG-APEX2-MAP1LC3BK51A

    Plasmid

    #175754

    Purpose

    Lentiviral vector expressing N-terminally tagged APEX2-FLAG-MAP1LC3B K51A

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    MAP1LC3B (MAP1LC3B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    K51A

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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