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pMAL-c2x-MT3

Plasmid

#32115

Depositor

David DeRosier, Chris Mercogliano, Cristina Risco Ortiz

Article

Diestra et al J Struct Biol. 2009 Mar;165(3):157-68. E

Insert

MT (3x) (Mt1 Mouse)

Tags

MBP

Expression

Bacterial

Promoter

P-lac

Available Since

Jan. 13, 2012

Availability

Academic Institutions and Nonprofits only

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lenti-NLS-mTagBFP2

Plasmid

#216128

Purpose

Lentiviral vector for expressing mTagBFP2 localizing to the nucleus

Depositor

Stefan Kubicek

Article

Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

Insert

EF1a-NuclearLocalizationSignal-mTagBFP2-WPRE

Use

Lentiviral

Expression

Mammalian

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_[Cox8a]x2_HaloTag7

Plasmid

#175529

Purpose

Expression of HaloTag7 at the inner membrane of mitochondria in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Cox8a-HaloTag7

Expression

Mammalian

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-flag-YTHDF2-N

Plasmid

#52302

Purpose

Flag-tagged YTHDF2 N-terminal domain, made by mutating to E384(GAA) to stop codon(TAA)

Depositor

Chuan He

Article

Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

Insert

YTHDF2 (YTHDF2 Human)

Tags

flag

Expression

Mammalian

Mutation

Changed E384 to stop codon

Promoter

T7

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
pTRANS_201

Plasmid

#91105

Purpose

Transformation, Type: T-DNA, Plant Selection: none, Viral Replication: BeYDV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

No Promoter

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pTRANS_100

Plasmid

#91198

Purpose

Transformation, Type: non-T-DNA, Plant Selection: none, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pDonor14-ShHELIX-KanR (CJT70)

Plasmid

#181793

Purpose

pDonor for ShHELIX containing 14bp of spacing between the I-AniI site and LE/RE and encoding a kanamycin resistance gene as cargo

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

KanR, R6K origin of replication

Expression

Bacterial

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pTRANS_260d

Plasmid

#91126

Purpose

Transformation, Type: T-DNA, Plant Selection: PvUbi2:bar, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

PvUbi2

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pRSM3

Plasmid

#49799

Purpose

ePathBrick vector for pathway engineering in E. coli.

Depositor

Mattheos Koffas

Article

Xu et al ACS Synth Biol. 2012 Jul 20;1(7):256-66.

Type

Empty backbone

Tags

S-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGC000

Plasmid

#48858

Purpose

Empty entry vector for creating GreenGate coding sequence modules.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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JP1520 PIK3CA E545K HA

Plasmid

#14571

Depositor

Joan Brugge

Article

Isakoff et al Cancer Res. 2005 Dec 1. 65(23):10992-100

Insert

PIK3CA E545K (PIK3CA Bovine)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Mutation

E545K

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
JP1520 PIK3CA H1047R HA

Plasmid

#14572

Depositor

Joan Brugge

Article

Isakoff et al Cancer Res. 2005 Dec 1. 65(23):10992-100

Insert

PIK3CA H1047R (PIK3CA Bovine)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Mutation

H1047R

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
pKC-Tol2

Plasmid

#85600

Purpose

Source of Tol2 transposase driven by a mini-CAGS promoter.

Depositor

Karl Clark, Scott Fahrenkrug

Article

Clark et al BMC Biotechnol. 2007 Jul 17;7:42.

Insert

Tol2 transposase

Expression

Mammalian

Promoter

mini-CAG

Available Since

Dec. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pRG203MX

Plasmid

#64521

Purpose

Integrative E. coli/S. cerevisiae shuttle vector

Depositor

Joerg Stelling

Article

Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pGGZ003

Plasmid

#48869

Purpose

Empty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA left border.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible plant transformation vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pENN496_miniCRISPRcharm_dSaCas9

Plasmid

#220837

Purpose

mini CRISPRcharm expression in mammalian cells

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

mini CRISPRcharm

Use

CRISPR

Tags

P2A-TagBFP

Expression

Mammalian

Mutation

N/A

Promoter

EFS

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSB-TRE-CRISPRoff-EF1A-TetOn

Plasmid

#203355

Purpose

Drug-inducible expression of CRISPRoff components needed for DNA methylation and gene repression with Sleeping Beauty backbone

Depositor

Jonathan Brunger

Article

Gil et al Bio Protoc. 2024 Jan 5;14(1):e4913. doi:

Insert

CRISPRoff-TetOn

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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gGBEv6.3-SpCas9

Plasmid

#202629

Purpose

vector for encoding an engineered glycosylase-based guanine base editor (gGBEv6.3) with SpCas9 nickase driven by EF1a promoter, guide RNA compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

Depositor

Huawei Tong

Article

Tong et al Natl Sci Rev. 2023 May 16;10(8):nwad143.

Insert

nSpCas9(D10A)-MPGv6.3

Expression

Mammalian

Mutation

MPGv6.3 = G163R, N169G, D175R, C178N, S198A, K202…

Promoter

hU6, EF1a, CBh

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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V5-LOV-Turbo-ERM

Plasmid

#199664

Purpose

expresses LOV-Turbo on the mammalian ER membrane, lentiviral vector

Depositor

Alice Ting

Article

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

LOV-Turbo

Use

Lentiviral

Tags

SEC61B and V5

Expression

Mammalian

Promoter

TRE3G

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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V5-LOV-Turbo-NLS

Plasmid

#199663

Purpose

expresses LOV-Turbo in the mammalian nucleus, lentiviral vector

Depositor

Alice Ting

Article

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

LOV-Turbo

Use

Lentiviral

Tags

NLS and V5

Expression

Mammalian

Promoter

TRE3G

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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