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GFP-PKCA WT

Plasmid

#195202

Purpose

Mammalian expression vector for GFP tagged PKCA.

Depositor

David Bryant, Peter Parker

Publication

Cameron et al Nat Struct Mol Biol. 2009 Jun;16(6):624-

Insert

PRKCA (PRKCA Human)

Expression

Mammalian

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2 hsp70l loxP-Non-FP-loxP-nCas9n-GFP

Plasmid

#158963

Purpose

Cre-dependet Cas9-GFP expression

Depositor

Michael Brand

Publication

Kesavan et al Development. 2020 Jun 8;147(11). pii: de

Inserts

hsp70l promoter
floxed Non-FP cassette
Cas9-GFP

Use

CRISPR and Cre/Lox

Available since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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IMPT-10314:GABBR2

Plasmid

#157851

Purpose

HA-3xFLAG-VFT-TMD-CC in pFastBac1 for Sf9 expression

Depositor

Vadim Cherezov, Raymond Stevens

Publication

Shaye et al Nature. 2020 Jun 17. pii: 10.1038/s41586

Insert

GABBR2 (GABBR2 Human)

Expression

Insect

Promoter

Polyhedrin

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pTRIPZ shTREX1

Plasmid

#127700

Purpose

Doxycyclin inducible shRNA knockdown of both human and mouse TREX1 gene

Depositor

Sandra Demaria

Publication

Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

Insert

shTREX1 (Trex1 Human, Mouse)

Use

Lentiviral

Expression

Mammalian

Available since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pTRIPZ shcGAS-Mmu

Plasmid

#127698

Purpose

Doxycyclin inducible shRNA knockdown of mouse cGAS gene

Depositor

Sandra Demaria

Publication

Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

Insert

shcGAS Mmu (Cgas Mouse)

Use

Lentiviral

Expression

Mammalian

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mNeonGreen-LEHD-NanoLuc

Plasmid

#98289

Purpose

caspase-9 BRET Sensor

Depositor

Maarten Merkx

Publication

den Hamer et al ACS Sens. 2017 Jun 23;2(6):729-734. doi:

Insert

mNeonGreen-LEHD-NanoLuc

Tags

His-tag

Expression

Mammalian

Promoter

CMV

Available since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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LARG mCherry sspB in pcDNA3.1

Plasmid

#90459

Purpose

RhoA mediated cell migration

Depositor

Narasimhan Gautam

Publication

O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

Inserts

LARG DH-PH (aa 766–1138) (ARHGEF12 Human)
mCherry
sspB

Expression

Mammalian

Mutation

R73Q

Promoter

CMV

Available since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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pIRES-hrGFP II-mTET1

Plasmid

#83569

Purpose

Expresses TET1 with a mutated catalytic domain in mammalian cells

Depositor

Jean-Pierre Issa

Publication

Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

Insert

Tet Methylcytosine Dioxygenase 1 (TET1 Human)

Tags

3X FLAG tag and hr GFP II

Expression

Mammalian

Mutation

catalytic domain mutant H1672D, D1674A

Promoter

CMV

Available since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEBB COMMD1 Flag

Plasmid

#74893

Purpose

Express COMMD1 in mammalian cells

Depositor

Ezra Burstein, Colin Duckett

Publication

Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

Insert

COMMD1 (COMMD1 Human)

Tags

Flag

Expression

Mammalian

Promoter

EF-1alpha promoter

Available since

June 23, 2016

Availability

Academic Institutions and Nonprofits only

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pFYF1328 EGFP Site#3

Plasmid

#47513

Purpose

human gRNA expression vector targeting EGFP

Depositor

Keith Joung

Publication

Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

Insert

gRNA-EGFP site 3

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pVC299 VEGF Site#2

Plasmid

#47506

Purpose

human gRNA expression vector targeting VEGF

Depositor

Keith Joung

Publication

Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

Insert

gRNA-VEGF site 2 (VEGFA Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGEX Lyn-SH2

Plasmid

#46452

Depositor

Bruce Mayer

Publication

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Lyn (LYN Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 106-213

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA5FRT PUR mGSK3A K148A

Plasmid

#35158

Depositor

Jim Woodgett

Publication

Doble et al Dev Cell. 2007 Jun;12(6):957-71.

Insert

GSK-3α (Gsk3a Mouse)

Expression

Mammalian

Mutation

K148A (dominant negative)

Promoter

CMV

Available since

May 18, 2012

Availability

Industry, Academic Institutions, and Nonprofits
bdSUMO-GFP-HiBit

Plasmid

#170989

Purpose

For bacterial expression of bdSUMO tagged muGFP with NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Publication

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-muGFP-HiBiT

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pKanCMV_mRuby3

Plasmid

#172854

Purpose

mRuby3 expressed under the CMV promotor

Depositor

Haining Zhong

Publication

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

mRuby3

Expression

Mammalian

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLVX-IRES-Gβγ-BERKY3

Plasmid

#158426

Purpose

BRET biosensor for detection of free Gβγ in lentiviral vector pLVX-IRES-Hyg

Depositor

Mikel Garcia-Marcos

Publication

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-3xYFP-GRK3ct

Use

Lentiviral

Promoter

CMV

Available since

Sept. 17, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-IRES-Gαi*-BERKY3

Plasmid

#158424

Purpose

BRET biosensor for detection of Gαi-GTP in lentiviral vector pLVX-IRES-Hyg

Depositor

Mikel Garcia-Marcos

Publication

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-3xYFP-KB1753-myc

Use

Lentiviral

Tags

myc

Promoter

CMV

Available since

Sept. 17, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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AAV-FLEX-EGFP-mir30(Scn9a-scrambled)

Plasmid

#79671

Purpose

Scrambled control for Cre-dependent knockdown of Scn9a (Nav1.7)

Depositor

Scott Sternson

Publication

Branco et al Cell. 2016 Jun 16;165(7):1749-61. doi: 1

Insert

EGFP

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pKO (Hyg)

Plasmid

#110087

Purpose

Suicide vector for generation of gene deletions in mycobacteria. Contains the two genes sacB and galK for negative selection.

Depositor

Markus Seeger

Publication

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Bacterial knockout generation

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pKO (Apr)

Plasmid

#110088

Purpose

Suicide vector for generation of gene deletions in mycobacteria. Contains the two genes sacB and galK for negative selection.

Depositor

Markus Seeger

Publication

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Bacterial knockout generation

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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