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HDM_RSV_B_OQ279862_F_N201T

Plasmid

#237362

Purpose

Expression plasmid for RSV B F from OQ279862

Depositor

Jesse Bloom

Article

Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

Insert

RSV B F human codon optimized from Genbank Accesion OQ27986

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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114_UBI_iCre_crystGFP

Plasmid

#171797

Purpose

Tol2-plasmid ubiquitously expressing iCre (ubiquitin promoter) along with eGFP selection marker (Crystallin promoter -Lens/Eyes-)

Depositor

Jean Giacomotto

Article

Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

Insert

optimised iCre under the control of the Ubiquitin promoter

Promoter

Ubiquitin

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pNL3.1-Erg

Plasmid

#182706

Purpose

Express Erg enhancer in mammalian cells

Depositor

Robert Rowe

Article

Wang et al Cell Rep. 2022 Apr 5;39(1):110587. doi:

Insert

Enhancer of Erythroblast transformation-specific-Related Gene (Erg Mouse)

Use

Luciferase

Available Since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pME-nCas9n-P2A-TagBFP2-pA

Plasmid

#176026

Purpose

Multisite gateway vector for expression of nCas9n with blue reporter. Parton lab clone ENT

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

nCas9n-P2A-TagBFP2

Use

Gateway Cloning

Available Since

Oct. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pME-Lifeact-mEos2-NS

Plasmid

#115549

Purpose

Multisite gateway vector for 5' tagging with Lifeact-mEos2. Used to make 115563. Parton lab clone IDA

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Lifeact-mEos2

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST-actc1b-ccdb-mKate2

Plasmid

#109731

Purpose

Multisite gateway destination vector for muscle specific expression of constructs tagged at the 3' end with mKate2

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Type

Empty backbone

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST-actc1b-mKate2-ccdb

Plasmid

#109732

Purpose

Multisite gateway destination vector for muscle specific expression of constructs tagged at the 5' end with mKate2

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Type

Empty backbone

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pME-nCas9n-P2A-mKate2-pA

Plasmid

#109547

Purpose

Multisite gateway vector for expression of nCas9n with bicistronic expression of mKate2

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

nCas9n and mKate2

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-ATG18

Plasmid

#109556

Purpose

Multisite gateway vector for 3' tagging with yeast ATG18 (PLEKHA3). Binds PtdIns(3,5P)2

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

yeast ATG18 (PLEKHA3)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pOPINK-ALG13 (OTU, aa 216-353)

Plasmid

#61414

Purpose

Expresses human ALG13 (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

ALG13 (Putative bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13) (ALG13 Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-215 and aa 354-1137.

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pHDM-SARS-CoV-2 Δ18

Plasmid

#164434

Purpose

Encodes SARS-CoV-2 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

Insert

SARS-CoV-2 Spike truncated to remove 18 amino acids (S )

Expression

Mammalian

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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PaPchE-AE

Plasmid

#242103

Purpose

Adenylase-epimerase didomain of the nonribosomal peptide synthetase PchE from Pseudomonas aeruginosa for the production of pyochelin

Depositor

Audrey Lamb

Article

Ronnebaum et al Biochemistry. 2025 Sep 16;64(18):3919-39

Insert

PaPchE-AE

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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PaPchF-AMT

Plasmid

#242104

Purpose

Adenylase-methyltransferase didomain of the nonribosomal peptide synthetase Pchf from Pseudomonas aeruginosa for the production of pyochelin

Depositor

Audrey Lamb

Article

Ronnebaum et al Biochemistry. 2019 Feb 12;58(6):665-678.

Insert

PaPchF-AMT

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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DriD-wHTH-DNABD

Plasmid

#237851

Purpose

The wHTH DNA binding domain used to understand the allosteric mechanism

Depositor

Maria Schumacher

Article

Cannistraci et al Commun Biol. 2025 Apr 29;8(1):679. doi:

Insert

DriD wHTH DNABD

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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DriD-3HB-mutant

Plasmid

#237852

Purpose

DriD 3HB surface mutant used to interrogate the allosteric mechanism

Depositor

Maria Schumacher

Article

Cannistraci et al Commun Biol. 2025 Apr 29;8(1):679. doi:

Insert

DriD 3HB mutant

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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DriD_78-327

Plasmid

#237853

Purpose

DriD missing the wHTH DNABD used to crystallize the apo protein

Depositor

Maria Schumacher

Article

Cannistraci et al Commun Biol. 2025 Apr 29;8(1):679. doi:

Insert

DriD 78-327

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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GFP SMG1 T3550A/S3556A

Plasmid

#199572

Purpose

Expresses mutated SMG1 fused to GFP

Depositor

Michael Kastan

Article

McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

Insert

SMG1 (SMG1 Human)

Tags

GFP

Expression

Mammalian

Mutation

T3550A and S3556A

Promoter

CMV

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pD864-LacZ backbone

Plasmid

#109384

Purpose

Empty plasmid of the pD864-LacZ construct. This is high copy (pUC), AmpR

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Type

Empty backbone

Expression

Bacterial

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pMC-cIAP1-FL

Plasmid

#20209

Depositor

Martin Holcik

Article

Zhao et al Cell Death Differ. 2009 Feb . 16(2):244-

Insert

cIAP1 3'UTR (BIRC2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pSHUT4-eYFP

Plasmid

#133094

Purpose

Agrobacterium T-DNA overexpression cassette comprising constitutive TEF-1alpha fused to reporter eYFP. Integrates near beta-tubulin gene in F. solani genome. Replace eYFP w/ XhoI BamHI

Depositor

Jens Laurids Sørensen

Article

Nielsen et al Fungal Biol Biotechnol. 2019 Dec 11;6:25

Insert

enhanced yellow flourescent protein

Use

Fungal expression

Promoter

TEF-1α

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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