We narrowed to 1,410 results for: pUC57

1. pIK86

   Plasmid

   #47933

   Purpose

   C. elegans codon-optimized Cas9 with 2xSV40 NLS sequences. It has a T7 promoter for in vitro transcription, germline-compatible 5'UTR, tbb-2 3'UTR and a polyA sequence.

   Depositor

   Iskra Katic

   Article

   Katic et al Genetics. 2013 Aug 26.

   Insert

   Cas9
   

   Use

   CRISPR and Unspecified

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Oct. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. APP_Halo_C_allele

   Plasmid

   #178137

   Purpose

   Donor vector for endogenous tagging of human APP at the C-terminus with halotag

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Insert

   Halotag (APP Human)
   

   Use

   Donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. FUS_Halo_C_allele_400

   Plasmid

   #161768

   Purpose

   Donor vector for endogenous tagging of human FUS at the C-terminus with halotag

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Insert

   halotag (FUS Human)
   

   Use

   Donor vector for endogenous tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTK398

   Plasmid

   #114714

   Purpose

   mAID-mClover-3FLAG tagging at NuMA's C-terminal locus

   Depositor

   Tomomi Kiyomitsu

   Article

   Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

   Insert

   mAID-mClover-3FLAG
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. p214_LTJ_1kbCD90.1Template

   Plasmid

   #82667

   Purpose

   1kb HDR template for murine CD90.1

   Depositor

   Lukas Jeker

   Article

   Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

   Insert

   1kb genomic DNA sequence from murine CD90.1
   

   Use

   Hdr template for gene editing

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p229_LTJ_2kbCD90.1Template

   Plasmid

   #82668

   Purpose

   2kb HDR template for murine CD90.1

   Depositor

   Lukas Jeker

   Article

   Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

   Insert

   2kb genomic DNA sequence from murine CD90.1
   

   Use

   Hdr template for gene editing

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p248_LTJ_2kbCD45.1Template

   Plasmid

   #82662

   Purpose

   2kb HDR template for murine CD45.1

   Depositor

   Lukas Jeker

   Article

   Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

   Insert

   2kb genomic DNA sequence from murine CD45.1
   

   Use

   Hdr template for gene editing

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p213_LTJ_160bpCD90.1Template

   Plasmid

   #82666

   Purpose

   160bp HDR template for murine CD90.1

   Depositor

   Lukas Jeker

   Article

   Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

   Insert

   160bp genomic DNA sequence from murine CD90.1
   

   Use

   Hdr template for gene editing

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p230_LTJ_4kbCD90.1Template

   Plasmid

   #82669

   Purpose

   4kb HDR template for murine CD90.1

   Depositor

   Lukas Jeker

   Article

   Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

   Insert

   4kb genomic DNA sequence from murine CD90.1
   

   Use

   Hdr template for gene editing

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p242_LTJ_1kbCD45.1Template

    Plasmid

    #82661

    Purpose

    1kb HDR template for murine CD45.1

    Depositor

    Lukas Jeker

    Article

    Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

    Insert

    1kb genomic DNA sequence from murine CD45.1
    

    Use

    Hdr template for gene editing

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. dg264_pENTR_ChronosCel

    Plasmid

    #66102

    Purpose

    Entry clone containing Chronos coding sequence (without stop codon), codon optimized for C. elegans, with synthetic introns, flanked by attL1 and attL2

    Depositor

    Dominique Glauser

    Article

    Schild et al Genetics. 2015 May 28. pii: genetics.115

    Insert

    Chronos
    

    Use

    Gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. CSF1R_Halo_C_allele

    Plasmid

    #178139

    Purpose

    Donor vector for endogenous tagging of human CSF1R at the C-terminus with halotag

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Halotag (CSF1R Human)
    

    Use

    Donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AA516

    Plasmid

    #231118

    Purpose

    Fragmid fragment: (2A Selection) CBE activity-based selection intron

    Depositor

    David Root, John Doench

    Insert

    PuroN'-intron-PuroC'
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AA498

    Plasmid

    #231119

    Purpose

    Fragmid fragment: (guide cassette) ABE activity-based selection CD274 positive control

    Depositor

    David Root, John Doench

    Insert

    sgCD274 + ABE splice-targeting sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AA505

    Plasmid

    #231120

    Purpose

    Fragmid fragment: (guide cassette) CBE activity-based selection CD274 positive control

    Depositor

    David Root, John Doench

    Insert

    sgCD274 + CBE splice-targeting sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AA509

    Plasmid

    #231116

    Purpose

    Fragmid fragment: (2A Selection) ABE activity-based selection intron

    Depositor

    David Root, John Doench

    Insert

    PuroN'-intron-PuroC'
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSA_0_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP1-10

    Plasmid

    #199487

    Purpose

    Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

    Depositor

    Robert Bryson-Richardson

    Article

    Miles et al bioRxiv 2023

    Insert

    Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP1-10
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSA_1_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP1-10

    Plasmid

    #199488

    Purpose

    Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

    Depositor

    Robert Bryson-Richardson

    Article

    Miles et al bioRxiv 2023

    Insert

    Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP1-10
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSA_2_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP1-10

    Plasmid

    #199489

    Purpose

    Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

    Depositor

    Robert Bryson-Richardson

    Article

    Miles et al bioRxiv 2023

    Insert

    Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP1-10
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKozak_Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP1-10

    Plasmid

    #199497

    Purpose

    Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

    Depositor

    Robert Bryson-Richardson

    Article

    Miles et al bioRxiv 2023

    Insert

    Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP1-10
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJMH020

    Plasmid

    #186633

    Purpose

    contains homology arms for the pilA (NIES39_C03030) locus of Arthrospira platensis

    Depositor

    Adam Arkin

    Article

    Jacob M. et al bioRxiv

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pJMH021

    Plasmid

    #186634

    Purpose

    contains homology arms for the NSI (NIES39_Q01230) locus of Arthrospira platensis

    Depositor

    Adam Arkin

    Article

    Jacob M. et al bioRxiv

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJMH022

    Plasmid

    #186635

    Purpose

    contains homology arms for the oscillin (NIES39_A01430) locus of Arthrospira platensis

    Depositor

    Adam Arkin

    Article

    Jacob M. et al bioRxiv

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. ITM2B_Halo_C_allele

    Plasmid

    #178160

    Purpose

    Donor vector for endogenous tagging of human ITM2B at the C-terminus with halotag

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Halotag (ITM2B Human)
    

    Use

    Donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. GCH1_Halo_N_allele

    Plasmid

    #178159

    Purpose

    Donor vector for endogenous tagging of human GCH1 at the N-terminus with halotag

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Halotag (GCH1 Human)
    

    Use

    Donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. CAA 659

    Plasmid

    #108684

    Purpose

    in vitro mRNA transcription

    Depositor

    Jon Lorsch

    Article

    Yourik et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

    Insert

    CAA repeat mRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDV

    Plasmid

    #128860

    Purpose

    Destination vector for modules assembly

    Depositor

    Gilles Charvin

    Article

    Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial, Mammalian, and Y…

    Mutation

    Promoter

    Available since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTK587

    Plasmid

    #114692

    Purpose

    SNAP-tagging at p150's C-terminal locus

    Depositor

    Tomomi Kiyomitsu

    Article

    Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

    Insert

    SNAP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTK426

    Plasmid

    #114694

    Purpose

    Nano-mCherry tagging at DHC's N-terminal locus

    Depositor

    Tomomi Kiyomitsu

    Article

    Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

    Insert

    Nano-mCherry
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. p243_LTJ_4kbCD45.1Template

    Plasmid

    #82663

    Purpose

    4kb HDR template for murine CD45.1

    Depositor

    Lukas Jeker

    Article

    Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

    Insert

    4kb genomic DNA sequence from murine CD45.1
    

    Use

    Hdr template for gene editing

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCrAb-km

    Plasmid

    #214199

    Purpose

    Expresses crRNA for genome editing in Acinetobacter baumannii

    Depositor

    Yu Wang

    Article

    Wang et al Curr Issues Mol Biol. 2024 Jan 5;46(1):5

    Insert

    crRNA-HDV
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    J23119

    Available since

    June 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. XLone-GFP

    Plasmid

    #96930

    Purpose

    Tunable and temporal expression control of GFP

    Depositor

    Xiaojun Lian

    Article

    Randolph et al Sci Rep. 2017 May 8;7(1):1549. doi: 10.1

    Insert

    Green Fluorescent Protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3GS

    Available since

    Aug. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. AICSDP-26:ST6GAL1-mEGFP

    Plasmid

    #101786

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human ST6GAL1

    Depositor

    Allen Institute for Cell Science

    Insert

    ST6GAL1 Homology Arms with linker-mEGFP (ST6GAL1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    Oct. 31, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. AICSDP-36: AAVS1-mEGFP (PGK)

    Plasmid

    #114404

    Purpose

    Homology arms and linker-mEGFP sequence for internal insertion of PGK-mEGFP at the AAVS1 safe harbor location in human cells as a reporter for cytoplasmic mEGFP

    Depositor

    Allen Institute for Cell Science

    Insert

    AAVS1 Homology Arms with PGK driven mEGFP (AAVS1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    Sept. 4, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. p236

    Plasmid

    #186182

    Purpose

    Shotgun Metagenomic Spike-in

    Depositor

    Karsten Zengler

    Article

    Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

    Insert

    synDNA 236
    

    Use

    Synthetic Biology; Cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. p126

    Plasmid

    #186176

    Purpose

    Shotgun Metagenomic Spike-in

    Depositor

    Karsten Zengler

    Article

    Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

    Insert

    synDNA 126
    

    Use

    Synthetic Biology; Cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. p146

    Plasmid

    #186178

    Purpose

    Shotgun Metagenomic Spike-in

    Depositor

    Karsten Zengler

    Article

    Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

    Insert

    synDNA 146
    

    Use

    Synthetic Biology; Cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. p156

    Plasmid

    #186179

    Purpose

    Shotgun Metagenomic Spike-in

    Depositor

    Karsten Zengler

    Article

    Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

    Insert

    synDNA 156
    

    Use

    Synthetic Biology and Unspecified; Cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. p136

    Plasmid

    #186177

    Purpose

    Shotgun Metagenomic Spike-in

    Depositor

    Karsten Zengler

    Article

    Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

    Insert

    synDNA 136
    

    Use

    Synthetic Biology; Cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. p166

    Plasmid

    #186180

    Purpose

    Shotgun Metagenomic Spike-in

    Depositor

    Karsten Zengler

    Article

    Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

    Insert

    synDNA 166
    

    Use

    Synthetic Biology; Cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge