-
Plasmid#45132DepositorInsertE-coli-pathways-QconCAT4
UseTags6XHisExpressionBacterialMutationPromoterT7Available sinceDec. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET29b_Qcat17
Plasmid#45145DepositorInsertE-coli-pathways-QconCAT17
UseTags6XHisExpressionBacterialMutationPromoterT7Available sinceDec. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPPC020.306
Plasmid#171148PurposeExpression of J3-BBa_J23117-mRFP and J306 scRNA on pBBR1-GmR plasmidDepositorInsertsJ306 scRNA
J3-BBa_J23117-mRFP
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 and J3-BBa_J23117Available sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTS2404-Tier1-PhCMV-NeoR
Plasmid#169496PurposeTier-1 vector encoding PhCMV-driven neomycin resistance gene NeoR (PhCMV-NeoR-pA).DepositorInsertPCMV-driven aminoglycoside 3'-phosphotransferase, APH 3' II
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM6_Ptac_BmHps_BmPhi_BsMdh
Plasmid#129103PurposeExpresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicusDepositorInsertBsMDH BmHPS BmPHI
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 11, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pChlr-8SE
Plasmid#52172PurposeEncodes module 8 with amino acids 12S-16E for recognition of nt 1GDepositorInsertPumilio homology domain amino acids 284-319 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12N to 12S and 16Q to 16E in module 8PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-1SE
Plasmid#52140PurposeEncodes module 1 with amino acids 12S-16E for recognition of nt 8GDepositorInsertPumilio homology domain amino acids 25-60 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 16Q to 16E in Module 1PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-2SE
Plasmid#52144PurposeEncodes module 2 with amino acids 12S-16E for recognition of nt 7GDepositorInsertPumilio homology domain amino acids 61-96 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12N to 12S and 16Q to 16E in Module 2PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-3SE
Plasmid#52148PurposeEncodes module 3 with amino acids 12S-16E for recognition of nt 6GDepositorInsertPumilio homology domain amino acids 97-132 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12C to 12S and 16Q to 16E in Module 3PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-4SYR
Plasmid#52158PurposeEncodes module 4 with amino acids 12S-13Y-16R for recognition of nt 5CDepositorInsertPumilio homology domain amino acids 133-168 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12N to 12S, 13H to 13Y, and 16Q to 16R in…PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-5SR
Plasmid#52162PurposeEncodes module 5 with amino acids 12S-16R for recognition of nt 4CDepositorInsertPumilio homology domain amino acids 169-204 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12C to 12S and 16Q to 16R in Module 5PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-6SE
Plasmid#52164PurposeEncodes module 6 with amino acids 12S-16E for recognition of nt 3GDepositorInsertPumilio homology domain amino acids 205-240 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12N to 12S and 16Q to 16E in Module 6PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJT2
Plasmid#98306PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoterDepositorInsertPTEF2>ScERG20N127W>TRPL3>PTEF1>AcNES1>TRPL41B
UseTagsExpressionYeastMutationERG20 N127WPromoterAvailable sinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pChlr-4SE
Plasmid#52152PurposeEncodes module 4 with amino acids 12S-16E for recognition of nt 5GDepositorInsertPumilio homology domain amino acids 133-168 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12N to 12S and 16Q to 16E in Module 4PromoterAvailable sinceMay 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-8SR
Plasmid#52174PurposeEncodes module 8 with amino acids 12S-16R for recognition of nt 1CDepositorInsertPumilio homology domain amino acids 284-319 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12N to 12S and 16Q to 16R in Module 8PromoterAvailable sinceMay 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-1SR
Plasmid#52142PurposeEncodes module 1 with amino acids 12S-16R for recognition of nt 8CDepositorInsertPumilio homology domain amino acids 25-60 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 16Q to 16R in Module 1PromoterAvailable sinceMay 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-7CQ
Plasmid#52167PurposeEncodes module 7 with amino acids 12C-16Q for recognition of nt 2ADepositorInsertPumilio homology domain amino acids 241-283 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12S to 12C and 16E to 16Q in Module 7PromoterAvailable sinceMay 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-7NQ
Plasmid#52169PurposeEncodes module 7 with amino acids 12N-16Q for recognition of nt 2UDepositorInsertPumilio homology domain amino acids 241-283 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12S to 12N and 16E to 16Q in module 7PromoterAvailable sinceMay 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-7SYR
Plasmid#52170PurposeEncodes module 7 with amino acids 12S-13Y-16R for recognition of nt 2CDepositorInsertPumilio homology domain amino acids 241-283 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 13N to 13Y and 16E to 16R in module 7PromoterAvailable sinceMay 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-1NQ
Plasmid#52141PurposeEncodes module 1 with amino acids 12N-16Q for recognition of nt 8UDepositorInsertPumilio homology domain amino acids 25-60 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12S to 12N in Module 1PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-2CQ
Plasmid#52143PurposeEncodes module 2 with amino acids 12C-16Q for recognition of nt 7ADepositorInsertPumilio homology domain amino acids 61-96 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12N to 12C in Module 2PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-2SR
Plasmid#52146PurposeEncodes module 2 with amino acids 12S-16R for recognition of nt 7CDepositorInsertPumilio homology domain amino acids 61-96 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12N to 12S and 16Q to 16R in Module 2PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-3NQ
Plasmid#52149PurposeEncodes module 3 with amino acids 12N-16Q for recognition of nt 6UDepositorInsertPumilio homology domain amino acids 97-132 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12C to 12N in Module 3PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-3SR
Plasmid#52150PurposeEncodes module 3 with amino acids 12S-16R for recognition of nt 6CDepositorInsertPumilio homology domain amino acids 97-132 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12C to 12S and 16Q to 16R in Module 3PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-4CQ
Plasmid#52151PurposeEncodes module 4 with amino acids 12C-16Q for recognition of nt 5ADepositorInsertPumilio homology domain amino acids 133-168 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12N to 12C in Module 4PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-4SR
Plasmid#52154PurposeEncodes module 4 with amino acids 12S-16R for recognition of nt 5CDepositorInsertPumilio homology domain amino acids 133-168 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12N to 12S and 16Q to 16R in Module 4PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-4CYQ
Plasmid#52155PurposeEncodes module 4 with amino acids 12C-13Y-16Q for recognition of nt 5ADepositorInsertPumilio homology domain amino acids 133-168 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12N to 12C and 13H to 13Y in Module 4PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-4NYQ
Plasmid#52157PurposeEncodes module 4 with amino acids 12N-13Y-16Q for recognition of nt 5UDepositorInsertPumilio homology domain amino acids 133-168 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 13H to 13Y in Module 4PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-5SE
Plasmid#52160PurposeEncodes module 5 with amino acids 12S-16E for recognition of nt 4GDepositorInsertPumilio homology domain amino acids 169-204 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12C to 12S and 16Q to 16E in Module 5PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-5NQ
Plasmid#52161PurposeEncodes module 5 with amino acids 12N-16Q for recognition of nt 4UDepositorInsertPumilio homology domain amino acids 169-204 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12C to 12N in Module 5PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-6CQ
Plasmid#52163PurposeEncodes module 6 with amino acids 12C-16Q for recognition of nt 3ADepositorInsertPumilio homology domain amino acids 205-240 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12N to 12C in Module 6PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-6SR
Plasmid#52166PurposeEncodes module 6 with amino acids 12S-16R for recognition of nt 3CDepositorInsertPumilio homology domain amino acids 205-240 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12N to 12S and 16Q to 16R in Module 6PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pY128
Plasmid#127713PurposeLevel 1 yeast shuttle vector for a transcription unit with A - D scars. Clone in with BsaI. Has a high-copy yeast replicon and KanMX selection.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-CrkII
Plasmid#50024PurposepENTR vector for mouse Crk-IIDepositorInsertCrk-II (Crk Mouse)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceDec. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
YIplac128-TEF2p-LDH-CYC1t
Plasmid#216120PurposeYeast integrative vector for constitutive expression of LDH at the LEU2 locusDepositorInsertLactate dehydrogenase (LDH Bovine)
UseTagsExpressionYeastMutationPromoterTEF2 promoterAvailable sinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-TDH3p-LDH-CYC1t
Plasmid#216121PurposeYeast integrative vector for constitutive expression of LDH at the TRP1 locusDepositorInsertLactate dehydrogenase (LDH Bovine, Bos taurus)
UseTagsExpressionYeastMutationPromoterTDH3 promoterAvailable sinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-TDH3p-CDC19-CYC1t
Plasmid#216122PurposeYeast integrative vector for constitutive expression of CDC19 at the TRP1 locusDepositorInsertPyruvate kinase 1
UseTagsExpressionYeastMutationPromoterTDH3 promoterAvailable sinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-GFPenhancer
Plasmid#202468PurposeExpresses the nanobody GFP enhancer after phosphate depletionDepositorInsertnanobody GFP enhancer
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-GFPminimizer
Plasmid#202469PurposeExpresses the nanobody GFP minimizer after phosphate depletionDepositorInsertnanobody GFP minimizer
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-H6antivenin
Plasmid#202470PurposeExpresses the nanobody H6 antivenin after phosphate depletionDepositorInsertnanobody H6 antivenin
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-1B5
Plasmid#202472PurposeExpresses the nanobody 1B5 after phosphate depletionDepositorInsertnanobody 1B5
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-2E7
Plasmid#202473PurposeExpresses the nanobody 2E7 after phosphate depletionDepositorInsertnanobody 2E7
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-3E3
Plasmid#202474PurposeExpresses the nanobody 3E3 after phosphate depletionDepositorInsertnanobody 3E3
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-C9BoNT-A
Plasmid#202475PurposeExpresses the nanobody C9 BoNT-A after phosphate depletionDepositorInsertnanobody C9 BoNT/A
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-E7BoNT-A
Plasmid#202476PurposeExpresses the nanobody E7 BoNT-A after phosphate depletionDepositorInsertnanobody E7 BoNT/A
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-JM3
Plasmid#202477PurposeExpresses the nanobody JM3 after phosphate depletionDepositorInsertnanobody JM3
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-J3
Plasmid#202478PurposeExpresses the nanobody J3 after phosphate depletionDepositorInsertnanobody J3
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-m36.4
Plasmid#202479PurposeExpresses the nanobody m36.4 after phosphate depletionDepositorInsertnanobody m36.4
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-Re5D06
Plasmid#202480PurposeExpresses the nanobody Re5D06 after phosphate depletionDepositorInsertnanobody Re5D06
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-Re9F06
Plasmid#202481PurposeExpresses the nanobody Re9F06 after phosphate depletionDepositorInsertnanobody Re9F06
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only