We narrowed to 767 results for: CRN;

1. pSLS.408

   Plasmid

   #187205

   Purpose

   Expresses retron Eco1 ncRNA v35, and Cas1 + Cas2

   Depositor

   Seth Shipman

   Article

   Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

   Inserts

   Retron Eco1 ncRNA v35
   Cas1 + Cas2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7/Lac

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. tdTomato-β-actin3'UTR2

   Plasmid

   #183717

   Purpose

   tdTomato with fragment nucleotides 441-676 of beta-actin 3'UTR inserted in 3'UTR

   Depositor

   Jeffrey Gerst

   Article

   Dasgupta et al Elife. 2023 May 30;12:e83584. doi: 10.75

   Insert

   tdTomato with fragment (nucleotides 441-676) of mouse Actb 3'UTR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   UbiC

   Available since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. tdTomato--β-actin CDS2

   Plasmid

   #183714

   Purpose

   tdTomato with fragment nucleotides 398-818 of beta-actin coding sequence inserted in 3'UTR

   Depositor

   Jeffrey Gerst

   Article

   Dasgupta et al Elife. 2023 May 30;12:e83584. doi: 10.75

   Insert

   tdTomato with fragment (nucleotides 398-818) of mouse Actb coding dsequence
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   UbiC

   Available since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. tdTomato-β-actin3'UTR1

   Plasmid

   #183716

   Purpose

   tdTomato with fragment nucleotides 1-440 of beta-actin 3'UTR inserted in 3'UTR

   Depositor

   Jeffrey Gerst

   Article

   Dasgupta et al Elife. 2023 May 30;12:e83584. doi: 10.75

   Insert

   tdTomato with fragment (nucleotides 1-440) of mouse Actb 3'UTR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   UbiC

   Available since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPPC018.AAV

   Plasmid

   #171146

   Purpose

   Expression of J1-BBa_J23117-mRFP and hAAVS1 scRNA on pRK2-GmR plasmid

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Inserts

   hAAVS1 scRNA
   J1-BBa_J23117-mRFP
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_J23119 and J1-BBa_J23117

   Available since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPPC030.306

   Plasmid

   #171151

   Purpose

   Mevalonate production pathway with J306 scRNA on pBBR1-GmR plasmid

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Inserts

   J306 scRNA
   J3-BBa_J23117-mvaES
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_J23119 and J3-BBa_J23117

   Available since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. 2Bc-T MBP_BoMoC(ed)_6xH

   Plasmid

   #186461

   Purpose

   Recombinant B. mori truncated R2 RT expression

   Depositor

   Kathleen Collins

   Article

   Upton et al Proc Natl Acad Sci U S A. 2021 Oct 19;11

   Insert

   B. mori truncated R2 RT
   

   Use

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. OPRM1-DuET

   Plasmid

   #213361

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   OPRM1 (OPRM1 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBBR1.cas9-NT

   Plasmid

   #197871

   Purpose

   Constitutive expression of Cas9 endonuclease, trancRNA and crRNA with a randomly generated spacer sequence (non-targeting)

   Depositor

   Baojun Wang

   Article

   Huan et al ACS Synth Biol. 2023 Mar 17;12(3):709-72

   Inserts

   trancCRNA
   Cas9
   crRNA (non-chromosomal (E. coli/S. flexneri)-targeting spacer sequence)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MRGPRD-DuET

    Plasmid

    #213340

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    MRGPRD (MRGPRD Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. NPY1R-DuET

    Plasmid

    #213354

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    NPY1R (NPY1R Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti6-AMPK alpha1 (1-312)

    Plasmid

    #162131

    Purpose

    Expression vector for constitutively active AMPK alpha 1

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2016 Apr;18(4):431-42. do

    Insert

    AMPK alpha1 (1-312) (PRKAA1 Rat)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    Available since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO5.U6.DR130(CasRX)_Empty.EFS.tRFP657

    Plasmid

    #212961

    Purpose

    Lentiviral plasmid for the cloning of mature gRNAs of the RfxCas13d (CasRx) CRISPR system. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.

    Depositor

    Roland Rad

    Article

    Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s

    Insert

    DR1_CasRx_backbone
    

    Use

    CRISPR and Lentiviral

    Tags

    TagRFP657

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO5.U6.DR130(CasRX)_Non-targeting sgRNA.EFS.tRFP657

    Plasmid

    #212962

    Purpose

    Lentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.

    Depositor

    Roland Rad

    Article

    Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s

    Insert

    DR1_CasRx_non-targeting spacer
    

    Use

    CRISPR and Lentiviral

    Tags

    TagRFP657

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO5.U6.DR130(CasRX)_GFP-targeting sgRNA.EFS.tRFP657

    Plasmid

    #212963

    Purpose

    Lentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.

    Depositor

    Roland Rad

    Article

    Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s

    Insert

    DR1_CasRx_GFP targeting spacer
    

    Use

    CRISPR and Lentiviral

    Tags

    TagRFP657

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-GFP-FUS-IDR

    Plasmid

    #183236

    Purpose

    Expression of eGFP fused to a intrinsically disordered regions of the FUS protein

    Depositor

    Jorge Ferrer

    Article

    Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

    Insert

    EGFP
    

    Use

    Tags

    FLAG tag and Fusion to an IDR from the FUS protei…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. UTS2R-DuET

    Plasmid

    #213386

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    UTS2R (UTS2R Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. OPN3-DuET

    Plasmid

    #213357

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    OPN3 (OPN3 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SSTR3-DuET

    Plasmid

    #213378

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    SSTR3 (SSTR3 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. P2RY11-DuET

    Plasmid

    #213364

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    P2RY11 (P2RY11 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge