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Plasmid#146023PurposeInsect Expression of oskar-3UTR-SRE1mutDepositorInsertoskar-3UTR-SRE1mut (osk Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1C-Fluc-nanos-SRE1,2mut_C
Plasmid#146019PurposeInsect Expression of nanos3UTR-SRE1,2mutDepositorInsertnanos3UTR-SRE1,2mut (nos Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1C-Fluc-nanos-SRE1mut_C
Plasmid#146020PurposeInsect Expression of nanos-3UTR-SRE1mutDepositorInsertnanos-3UTR-SRE1mut (nos Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1C-Fluc-nerfin-HSL_E
Plasmid#146228PurposeInsect Expression of Nerfin-3UTRDepositorInsertNerfin-3UTR (nerfin-1 Fly)
UseTagsExpressionInsectMutationNucleotide mutations A>T 4387 and A>T 4955 …PromoterAvailable sinceFeb. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPLN-hFluc-DM-HA-GFP11-KDEL
Plasmid#177723PurposeDM Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminusDepositorInserthFlucDM
UseTagsGFP11, HA, KDEL, and Prolactin Signal SequenceExpressionBacterial and MammalianMutationR188Q, R261Q (Destabilized Mutant)PromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPLN-hFluc-WT-eGFP-KDEL
Plasmid#177727PurposeFirefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention and fused to eGFP at the C-terminusDepositorInserthFluc
UseTagsKDEL, Prolactin Signal Sequence, and eGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
OA-1052B (Ubiq-Fluc_Ubiq-Rluc)
Plasmid#132426PurposeExpress Firefly Luciferase-eGFP and Renilla Luciferase under Ubiquitin-63E promoterDepositorInsertsUbiq promoter
firefly luciferase
T2A-eGFP
Renilla Luciferase-SV40-P10
reversed ubiq promoter
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL4.13/AUG(30)-FFLuc-3XFLAG
Plasmid#127336PurposeExpress truncated 3xFLAG tagged firefly luciferase (FFLuc) from AUG at codon 30DepositorInsertFirefly luciferase
UseTags3xFLAGExpressionMammalianMutationPromoterSV40Available sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL4.13/AUG(59)-FFLuc-3XFLAG
Plasmid#127337PurposeExpress truncated 3xFLAG tagged firefly luciferase (FFLuc) from AUG at codon 59DepositorInsertFirefly luciferase
UseTags3xFLAGExpressionMammalianMutationPromoterSV40Available sinceJune 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTH862-CEN-slowmaxRLuc/minCFLuc
Plasmid#115371PurposeExpresses translation initiation-controlled Renilla luciferase and translation elongation-controlled firefly luciferaseDepositorInsertsFirefly Luciferase (codon minimised)
Gcn4 5'-UTR + Renilla luciferase (codon optimised)
UseTagsExpressionYeastMutationAll codons have been changed for the most favoura…PromoterADH1 and TDH3Available sinceJune 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTH825-CEN-minRLuc/slowstaCFLuc
Plasmid#115370PurposeExpresses translation elongation-controlled Renilla luciferase and translation initiation-controlled firefly luciferaseDepositorInsertsGcn4 5'-UTR + Firefly Luciferase
Renilla luciferase (codon minimised)
UseTagsExpressionYeastMutationAll codons have been changed for the least favour…PromoterADH1 and TDH3Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTH748-CEN-RLuc/min8maxCFLuc
Plasmid#38214DepositorInsertsFirefly Luciferase
Renilla luciferase
UseTagsExpressionYeastMutationThe first eight codons of the original FLuc gene …PromoterADH1 and TDH3 (=GDP)Available sinceNov. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTH753-CEN-RLuc/min346maxCFLuc
Plasmid#38219DepositorInsertsFirefly Luciferase
Renilla luciferase
UseTagsExpressionYeastMutationThe first 346 codons of the original FLuc gene we…PromoterADH1 and TDH3 (=GPD)Available sinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTH747-CEN-RLuc/min4maxCFLuc
Plasmid#38213DepositorInsertsFirefly Luciferase
Renilla luciferase
UseTagsExpressionYeastMutationThe first four codons of the original FLuc gene w…PromoterADH1 and TDH3 (=GDP)Available sinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
RCAS (A) Flu cTbx4 (CT#700)
Plasmid#13925DepositorInsertTbx4 (TBX4 Chicken)
UseRetroviral; Avian expressionTagsFlu (3xHA)ExpressionMutationPromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-Venus-Fluc (puro)
Plasmid#140328PurposeLentivirus for expression of Venus-Fluc (firefly luciferase) fusion proteinDepositorInsertVenus-Firefly Luciferase (luc2)
UseLentiviralTagsExpressionMutationPromoterhuman PGK promoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMuLE_EXPR_CMV-eGFP_TOP-NLuc1.1_12GLI-FLuc_CBF-GLuc
Plasmid#113862PurposeTriple pathway reporter, 3P-Luc; wnt-NLuc1.1, hedgehog-FLuc, notch-GLuc; plus CMV-eGFP. Gateway expression vector for lentivirus generation.DepositorInsertsCMV-eGFP
TOP-NLuc1.1
12GLI-Fluc
CBF-GLuc
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNL(CMV)Fluc/CMV/WPREdU3
Plasmid#136881PurposeReporter vector expression firefly luciferaseDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2092 - [1-2] ENTR - Fluor - mNeon(PATC)
Plasmid#159853PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - mNeon(PATC)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-FLuc-WPRE-HGHpA
Plasmid#87951PurposeRecombinant single-stranded AAV transfer vector expressing Firefly Luciferase under the EF1a promoterDepositorInsertFirefly Luciferase
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only