We narrowed to 4,994 results for: mos

1. pcDNA3-LifeAct-FRB-Venus-173-N

   Plasmid

   #87616

   Purpose

   FRB-tagged N-terminal Venus (YFP) fragment for BiFC; localizes to actin cytoskeleton.

   Depositor

   Jin Zhang

   Article

   Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

   Insert

   LifeAct-FRB-Venus173-N
   

   Tags

   17-aa sequence from Abp140 (aka "Lifeact&quo…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAIP-hXRCC1wt-eGFP

   Plasmid

   #206032

   Purpose

   Mammalian expression of hXRCC1wt coupled to eGFP under the control of a CAG promoter

   Depositor

   Primo Schaer

   Article

   Schwarz et al Nat Commun. 2024 Jan 2;15(1):184. doi: 1

   Insert

   hXRCC1
   

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-sapphireCKAR

   Plasmid

   #118468

   Purpose

   cp-T-sapphire-based single-color biosensor for monitoring Protein Kinase C activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   sapphireCKAR
   

   Tags

   6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-ExRai-AktAR

   Plasmid

   #118462

   Purpose

   cpGFP-based excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   ExRai-AktAR
   

   Tags

   6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-RAB-AKARev

   Plasmid

   #118478

   Purpose

   ddRFP-based single-color biosensor for monitoring PKA (Protein Kinase A) activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   RAB-AKARev
   

   Tags

   6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPHT-PKA

   Plasmid

   #60936

   Purpose

   Fluorescent FPX sensor for cAMP

   Depositor

   Anne Marie Quinn

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   PKA FPX Sensor
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. sgLenti

   Plasmid

   #105996

   Purpose

   single sgRNA expression vector

   Depositor

   Mike McManus

   Article

   Boettcher et al Nat Biotechnol. 2018 Jan 15. pii: nbt.40

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral; S.pyogenes sgrna expressio…

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-sapphireCKAR(T/A)

   Plasmid

   #118469

   Purpose

   Negative-control mutant for sapphireCKAR biosensor.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   sapphireCKAR(T/A)
   

   Tags

   6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Contains Thr-to-Ala mutation in substrate sequenc…

   Promoter

   CMV

   Available Since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+)-blueAKAR(T/A)

   Plasmid

   #118471

   Purpose

   Negative-control mutant for blueAKAR biosensor.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   blueAKAR(T/A)
   

   Tags

   6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Contains Thr-to-Ala mutation in substrate sequenc…

   Promoter

   CMV

   Available Since

   April 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-blueCKAR

    Plasmid

    #118472

    Purpose

    cpBFP-based single-color biosensor for monitoring Protein Kinase C activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    blueCKAR
    

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV Ef1a DIO GeNL_SS hGtACR1

    Plasmid

    #233511

    Purpose

    Luciferase opsin fusion protein (luminopsin) for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Ikefuama et al iScience. 2025 Feb 13;28(3):112022. doi:

    Inserts

    mNeonGreen
    ISN-IE
    hGtACR1
    

    Use

    AAV

    Tags

    Gaussia luciferase signal sequence

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-Lyn-RAB-EKARev

    Plasmid

    #118477

    Purpose

    ddRFP-based single-color ERK biosensor targeted to the plasma membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    Lyn-RAB-EKARev
    

    Tags

    N-terminal targeting sequence from Lyn-kinase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-Lifeact-μDpTT

    Plasmid

    #182322

    Purpose

    Actin-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

    Depositor

    Jin Zhang

    Article

    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

    Insert

    Lifeact-μDronpa-TagRFP-T
    

    Tags

    N-terminal 17 amino acids from budding yeast Abp1…

    Expression

    Mammalian

    Mutation

    Dronpa contains H193T mutation.

    Promoter

    CMV

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+)-ExRai-AKAR-NES

    Plasmid

    #118407

    Purpose

    Cytosol-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    ExRai-AKAR-NES
    

    Tags

    Nuclear export signal (NES)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. Rix-PGK-Tom-W

    Plasmid

    #25813

    Depositor

    Jozsef Kiss, Patrick Salmon

    Article

    Dayer et al Brain. 2007 Nov . 130(Pt 11):2962-76.

    Insert

    Rix-PGK-Tom-W
    

    Use

    Lentiviral

    Mutation

    tdTomato from Tsien lab

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1(+)-sapphireAKAR-NLS

    Plasmid

    #118467

    Purpose

    cp-T-sapphire-based single-color PKA biosensor targeted to the nucleus.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    sapphireAKAR-NLS
    

    Tags

    Nuclear localization signal (NLS)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pENTR SD Age HsSAS-6

    Plasmid

    #46381

    Purpose

    For expression of C-terminal fusions.

    Depositor

    Pierre Gonczy

    Article

    Kitagawa et al Cell. 2011 Feb 4;144(3):364-75. doi: 10.

    Insert

    SAS-6 (SASS6 Human)
    

    Use

    Entry vector

    Available Since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-Lyn-sapphireAKAR

    Plasmid

    #118466

    Purpose

    cp-T-sapphire-based single-color PKA biosensor targeted to the plasma membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    Lyn-sapphireAKAR
    

    Tags

    N-terminal targeting sequence from Lyn kinase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1(+)-sapphireAKAR(T/A)

    Plasmid

    #118465

    Purpose

    Negative-control mutant for sapphireAKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    sapphireAKAR(T/A)
    

    Tags

    6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1(+)-ExRai-AKAR-Kras

    Plasmid

    #118408

    Purpose

    Plasma membrane-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    ExRai-AKAR-Kras
    

    Tags

    C-terminal targeting sequence from Kras

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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