We narrowed to 5,266 results for: mos

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pcDNA3.1(+)-ExRai-AKAR-NES

Plasmid

#118407

Purpose

Cytosol-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-AKAR-NES

Tags

Nuclear export signal (NES)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV Ef1a DIO GeNL_SS hGtACR1

Plasmid

#233511

Purpose

Luciferase opsin fusion protein (luminopsin) for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Ikefuama et al iScience. 2025 Feb 13;28(3):112022. doi:

Inserts

mNeonGreen
ISN-IE
hGtACR1

Use

AAV

Tags

Gaussia luciferase signal sequence

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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Rix-PGK-Tom-W

Plasmid

#25813

Depositor

Jozsef Kiss, Patrick Salmon

Article

Dayer et al Brain. 2007 Nov . 130(Pt 11):2962-76.

Insert

Rix-PGK-Tom-W

Use

Lentiviral

Mutation

tdTomato from Tsien lab

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-sapphireAKAR-NLS

Plasmid

#118467

Purpose

cp-T-sapphire-based single-color PKA biosensor targeted to the nucleus.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

sapphireAKAR-NLS

Tags

Nuclear localization signal (NLS)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-μDpTT

Plasmid

#182321

Purpose

Plasma membrane-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

Depositor

Jin Zhang

Article

Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

Insert

Lyn-μDronpa-TagRFP-T

Tags

N-terminal targeting sequence from Lyn kinase

Expression

Mammalian

Mutation

Dronpa contains H193T mutation.

Promoter

CMV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CYP450-μDpTT

Plasmid

#182325

Purpose

Endoplasmic reticulum-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

Depositor

Jin Zhang

Article

Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

Insert

DAKAP-μDronpa-TagRFP-T

Tags

N-terminal 27 amino acids from CYP450

Expression

Mammalian

Mutation

Dronpa contains H193T mutation.

Promoter

CMV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lifeact-μDpTT

Plasmid

#182322

Purpose

Actin-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

Depositor

Jin Zhang

Article

Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

Insert

Lifeact-μDronpa-TagRFP-T

Tags

N-terminal 17 amino acids from budding yeast Abp1…

Expression

Mammalian

Mutation

Dronpa contains H193T mutation.

Promoter

CMV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR SD Age HsSAS-6

Plasmid

#46381

Purpose

For expression of C-terminal fusions.

Depositor

Pierre Gonczy

Article

Kitagawa et al Cell. 2011 Feb 4;144(3):364-75. doi: 10.

Insert

SAS-6 (SASS6 Human)

Use

Gateway Cloning

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-sapphireAKAR

Plasmid

#118466

Purpose

cp-T-sapphire-based single-color PKA biosensor targeted to the plasma membrane.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

Lyn-sapphireAKAR

Tags

N-terminal targeting sequence from Lyn kinase

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR Age HsSAS-6 ST

Plasmid

#46382

Purpose

For expression of N-terminal fusions.

Depositor

Pierre Gonczy

Article

Kitagawa et al Cell. 2011 Feb 4;144(3):364-75. doi: 10.

Insert

SAS-6 (SASS6 Human)

Use

Gateway Cloning

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-3xflagGREB1b

Plasmid

#112222

Purpose

mammalian expression of flag tagged GREB1b

Depositor

Craig Burd

Article

Haines et al Endocr Relat Cancer. 2018 Jul;25(7):735-

Insert

GREB1b (GREB1 Human)

Tags

3x flag

Expression

Mammalian

Available Since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-FRB-Venus-173-N

Plasmid

#87615

Purpose

FRB-tagged N-terminal Venus (YFP) fragment for BiFC; localizes to plasma membrane.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

Lyn-FRB-Venus173-N

Tags

N-terminal targeting sequence from Lyn kinase

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pInducer21 Flag-Sesn2 419A/422A/426A

Plasmid

#61870

Purpose

Dox inducible lentivirial expression of Flag-Sesn 419A/422A/426A (mouse) mutant

Depositor

Ming Li

Article

Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

Insert

Sesn2 (Sesn2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

419A/422A/426A

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only
pLJM1 Flag-Sesn2 419A/422A/426A

Plasmid

#61872

Purpose

Lentivirial expression of Flag-Sesn 419A/422A/426A (mouse) mutant

Depositor

Ming Li

Article

Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

Insert

Sesn2 (Sesn2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

419A/422A/426A

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-Lyn-FRB-DMVF-181-N

Plasmid

#87609

Purpose

FRB-tagged N-terminal Dronpa fragment for BiFC; localizes to plasma membrane.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

Lyn-FRB-DMVF181-N

Tags

N-terminal targeting sequence from Lyn kinase

Expression

Mammalian

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3-LifeAct-FRB-DMVF-181-N

Plasmid

#87610

Purpose

FRB-tagged N-terminal Dronpa fragment for BiFC; localizes to actin cytoskeleton.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

LifeAct-FRB-DMVF181-N

Tags

17-aa sequence from Abp140 (aka "Lifeact&quo…

Expression

Mammalian

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-RAB-EKARev

Plasmid

#118477

Purpose

ddRFP-based single-color ERK biosensor targeted to the plasma membrane.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

Lyn-RAB-EKARev

Tags

N-terminal targeting sequence from Lyn-kinase

Expression

Mammalian

Promoter

CMV

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-neo-C11orf51-3xFlag (2765)

Plasmid

#39859

Depositor

Jonathon Pines

Article

Mansfeld et al Nat Cell Biol. 2011 Sep 18;13(10):1234-4

Insert

C11orf51 (ANAPC15 Human)

Tags

3xFlag

Expression

Mammalian

Available Since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only
pPHT-PIP2

Plasmid

#60937

Purpose

Fluorescent FPX sensor for PIP2

Depositor

Anne Marie Quinn

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

PIP2 dimerization dependent sensor RA+B monomers

Tags

dimerization dependent fluorescent protein monome…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ExRai-AktAR(T/A)

Plasmid

#118463

Purpose

Negative-control mutant for ExRai-AktAR biosensor.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-AktAR(T/A)

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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