We narrowed to 639 results for: pBR322

1. pJH806_pJH_LldR_Pcon-sfGFP.1TAG

   Plasmid

   #163737

   Purpose

   Constitutive expression of green fluorescent protein encoding one stop codon

   Depositor

   Matthew Bennett

   Article

   Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

   Insert

   lldr gfp.1TAG
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   gfp.N39TAG

   Promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJH721_pJH_LldR_PlldR-sfGFP.20TAG

   Plasmid

   #163729

   Purpose

   Lactate-inducible expression of green fluorescent protein encoding twenty amber codons

   Depositor

   Matthew Bennett

   Article

   Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

   Insert

   lldr gfp.20TAG
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   gfp with all 20 leucine codons mutated to amber

   Promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJH727_pJH_LldR_PlldR-sfGFP.10TAG

   Plasmid

   #163731

   Purpose

   Lactate-inducible expression of green fluorescent protein encoding ten amber codons

   Depositor

   Matthew Bennett

   Article

   Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

   Insert

   lldr gfp.10TAG
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   gfp with first 10 leucine codons mutated to amber

   Promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJH774_pJH_LldR_PlldR-sfGFP.1opal

   Plasmid

   #163733

   Purpose

   Lactate-inducible expression of green fluorescent protein encoding one opal codon

   Depositor

   Matthew Bennett

   Article

   Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

   Insert

   lldr gfp.1TGA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   gfp with first leucine codon mutated to amber

   Promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJH775_pJH_LldR_PlldR-sfGFP.2opal

   Plasmid

   #163734

   Purpose

   Lactate-inducible expression of green fluorescent protein encoding two opal codons

   Depositor

   Matthew Bennett

   Article

   Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

   Insert

   lldr gfp.2TGA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   gfp with first two leucine codons mutated to amber

   Promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJH28_pBad_AraC_Para-sfGFP.3TAG

   Plasmid

   #163723

   Purpose

   Arabinose-inducible expression of green fluorescent protein encoding three amber codons

   Depositor

   Matthew Bennett

   Article

   Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

   Insert

   araC gfp.3TAG
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   gfp.N39TAG,N135TAG,Y151TAG

   Promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJH626_pJH_LldR_PlldR-sfGFP.1TAG

   Plasmid

   #163725

   Purpose

   Lactate-inducible expression of green fluorescent protein encoding one amber codon

   Depositor

   Matthew Bennett

   Article

   Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

   Insert

   lldr gfp.1TAG
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   gfp.N39TAG

   Promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJH627_pJH_LldR_PlldR-sfGFP.2TAG

   Plasmid

   #163726

   Purpose

   Lactate-inducible expression of green fluorescent protein encoding two amber codons

   Depositor

   Matthew Bennett

   Article

   Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

   Insert

   lldr gfp.2TAG
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   gfp.N39TAG,Y135TAG

   Promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJH628_pJH_LldR_PlldR-sfGFP.3TAG

   Plasmid

   #163727

   Purpose

   Lactate-inducible expression of green fluorescent protein encoding three amber codons

   Depositor

   Matthew Bennett

   Article

   Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

   Insert

   lldr gfp.3TAG
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   gfp.N39TAG,N135TAG,Y151TAG

   Promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJH578

    Plasmid

    #172162

    Purpose

    pylBCD variant in pBAD backbone

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    Para (pylBCD 3f2_v2); AraC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    PylB(N61S, E84K, E122K, E178K, G188R, E251D) PylC…

    Promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJH767

    Plasmid

    #172172

    Purpose

    PylB overexpression and purification

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    PT7/Lac-[His6-TEV-SUMO-PylB.WT]
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylB]

    Promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJH857

    Plasmid

    #172175

    Purpose

    PylB overexpression and purification

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    PT7/Lac-[His6-TEV-SUMO-PylB.WTopt]
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBopt]

    Promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJH223

    Plasmid

    #172151

    Purpose

    pylBCD variant in pCDF backbone

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    PT7/lac (pylBCD 34B_sub-pop5); LacI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    PylB(N61S, E178K, E251D) PylD(A252V)

    Promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJH224

    Plasmid

    #172152

    Purpose

    pylBCD variant in pCDF backbone

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    PT7/lac (pylBCD 40C_sub-pop6); LacI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    PylB(N61S, E175K, E178K)

    Promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJH463

    Plasmid

    #172155

    Purpose

    pylBCD variant in pBAD backbone

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    Para (pylBCD 36A_sub-pop1); AraC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    PylB(N61S, E122K, E178K, G188R)

    Promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJH573

    Plasmid

    #172157

    Purpose

    pylBCD variant in pBAD backbone

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    Para (Untagged pylBCD); AraC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon-optimized Methanosarcina acetivorans pylBCD

    Promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJH574

    Plasmid

    #172158

    Purpose

    pylBCD variant in pBAD backbone

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    Para (pylBCD 36A_sub-pop2); AraC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    PylB(N61S, E84K, E122K) PylC(E316D)

    Promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJH575

    Plasmid

    #172159

    Purpose

    pylBCD variant in pBAD backbone

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    Para (pylBCD 34B_sub-pop3); AraC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Sumo(S6L) PylB(N61S, E178K, G188R, E251D) PylD(A2…

    Promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJH576

    Plasmid

    #172160

    Purpose

    pylBCD variant in pBAD backbone

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    Para (pylBCD 34B_sub-pop4); AraC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    PylB(N61S, E175K, E178K, E251D) PylD(A252V)

    Promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJH60

    Plasmid

    #172142

    Purpose

    Microscopy inclusion body assay

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    pCDF.CD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    PylCD partial operon (PylB deleted to assess impa…

    Promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge