We narrowed to 3,069 results for: FRI

1. pLJC2-TK2-3xFLAG

   Plasmid

   #217427

   Purpose

   Lentiviral overexpression of human TK2

   Depositor

   Jason Cantor

   Article

   Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

   Insert

   TK2 (TK2 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Insert sequence is a codon-optimized gBLOCK (IDT)

   Promoter

   CMV

   Available since

   Jan. 31, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiCRISPR-v1-sgTYMS

   Plasmid

   #217430

   Purpose

   Lentiviral vector expressing Cas9 and an sgRNA targeting human TYMS

   Depositor

   Jason Cantor

   Article

   Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

   Insert

   sgRNA targeting TYMS (TYMS Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 31, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLentiCRISPR-v1-sgTK2_4

   Plasmid

   #217431

   Purpose

   Lentiviral vector expressing Cas9 and an sgRNA targeting human TK2

   Depositor

   Jason Cantor

   Article

   Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

   Insert

   sgRNA 4 targeting TK2 (TK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 31, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLentiCRISPR-v1-sgTK2_5

   Plasmid

   #217432

   Purpose

   Lentiviral vector expressing Cas9 and an sgRNA targeting human TK2

   Depositor

   Jason Cantor

   Article

   Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

   Insert

   sgRNA 5 targeting TK2 (TK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 31, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLentiCRISPR-v1-sgSHMT2

   Plasmid

   #217435

   Purpose

   Lentiviral vector expressing Cas9 and an sgRNA targeting human SHMT2

   Depositor

   Jason Cantor

   Article

   Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

   Insert

   sgRNA targeting SHMT2 (SHMT2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 31, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-Fgß-FIVAR-ELP-HIS-ybbR

   Plasmid

   #169136

   Purpose

   E. coli expression of FIVAR domain containing Fgß tag, ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.

   Depositor

   Michael Nash

   Article

   Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

   Insert

   FIVAR (found in various architectures)
   

   Use

   Tags

   3xELP linker, 6x Histag, Fgβ peptide, and ybbr ta…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS2-Opto-Alk3

   Plasmid

   #207614

   Purpose

   Zebrafish Alk3 BMP receptor kinase domain fused to VfLOV

   Depositor

   Patrick Müller

   Article

   Rogers et al Elife. 2020 Nov 11;9:e58641. doi: 10.755

   Insert

   Alk3 (bmpr1aa) kinase domain + VfLOV domain (bmpr1aa Zebrafish, Vaucheria frigida)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-MUC2 D1 with cleavable His tag

   Plasmid

   #181813

   Purpose

   Expresses human MUC2 D1 with cleavable His tag, residues 21-389

   Depositor

   Deborah Fass

   Article

   Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

   Insert

   Muc2 D1 (MUC2 Human)
   

   Use

   Tags

   6xHistadine followed by TEV cleavage site

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pgRNA_CV2

   Plasmid

   #167165

   Purpose

   pgRNA_CV2 is derived from gRNA_cloning vector (Addgene plasmid ID: 41824) by adding about 80 bps from the sgRNA sequence as well as an AgeI site. In the literature, sgRNA_AL is used as an alias.

   Depositor

   Alexander Loewer

   Article

   Sheng et al Cell Rep. 2019 Apr 2;27(1):48-58.e7. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET15b-GreBdm-D41N

    Plasmid

    #129691

    Purpose

    express E. coli GreB E82C/C68S/D41N

    Depositor

    Jeff Gelles

    Article

    Tetone et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

    Insert

    GreB E82C C68S D41N (greB E. coli)
    

    Use

    Tags

    his6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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