Addgene

We narrowed to 11,654 results for: cel.2

Showing: 4171 - 4200 of 11654 results

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KA1396_pGL4-UBCmin-luc2/Klf4-E'

Plasmid

#124187

Purpose

Luciferase reporter driven by the Klf4 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Klf4 enhancer

Use

Luciferase

Tags

Expression

Mutation

Promoter

UBCmin

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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KA2626_pGL4-UBCmin-luc2/Klf2-D'

Plasmid

#124199

Purpose

Luciferase reporter driven by the Klf2 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Klf2 enhancer

Use

Luciferase

Tags

Expression

Mutation

Promoter

UBCmin

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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KA1401_pGL4-UBCmin-luc2/Klf4-F'

Plasmid

#124188

Purpose

Luciferase reporter driven by the Klf4 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Klf4 enhancer

Use

Luciferase

Tags

Expression

Mutation

Promoter

UBCmin

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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KA1395_pGL4-UBCmin-luc2/Klf4-B'

Plasmid

#124186

Purpose

Luciferase reporter driven by the Klf4 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Klf4 enhancer

Use

Luciferase

Tags

Expression

Mutation

Promoter

UBCmin

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV5 Flag-Par6 delta 250-295 Flag tagged

Plasmid

#24646

Depositor

Jeff Wrana

Article

Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

Insert

Par6c (Pard6a Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Deletion of the PDZ domain of Par6c (aa 250-295)

Promoter

Available since

June 2, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA5-FRT-TO-NUbo-FLAG

Plasmid

#212797

Purpose

Constitutive/Doxycycline-inducible expression of NUbo-FLAG in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

NUbo

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pBI-CMV1-mir181b-1~125-CMV2-mir1

Plasmid

#164671

Purpose

Express miR-181b-1~125a and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-181b-1~125a (MIR181B1 Human)
miR-1-1 (MIR1-1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-HisFlag-rUSP19ER-CAmut

Plasmid

#155237

Purpose

for mammalian overexpression of the mutated ER isoform of rat USP19

Depositor

Simon Wing

Article

Lu et al Mol Cell Biol. 2009 Jan;29(2):547-58. do

Insert

USP19 (ER isoform) (Usp19 Rat)

Use

Tags

6XHis-3XFlag

Expression

Mammalian

Mutation

Cys(545) to Ala

Promoter

CMV

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19-eGFP-P2A-dTAG-Ing3

Plasmid

#170754

Purpose

Donor plasmid for eGFP-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Ing3 locus

Depositor

Kristian Helin

Article

Damhofer et al STAR Protoc. 2021 Jun 2;2(2):100575. doi

Insert

eGFP-P2A-2xHA-dTAG

Use

Mouse Targeting

Tags

eGFP-P2A-2xHA-dTAG

Expression

Mutation

Phenylalanine 36 to valine in FKBP12

Promoter

none

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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p2xp53_RE

Plasmid

#118053

Purpose

GBPart - Operator/DNA Response element - 2 copies of the p53 DNA binding motif

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

2 copies of the p53 DNA binding motif

Use

Synthetic Biology; Domestication of dna parts for…

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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KHBD00552

Plasmid

#39647

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG10699 (Lim3 Fly)

Use

Gateway donr vector

Tags

Expression

Mutation

Promoter

none

Available since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00091

Plasmid

#34247

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG1071 (E2f2 Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pBa.HALO-KIF3A tail 419-702

Plasmid

#193924

Purpose

Mammalian expression of Halo-tagged Kif 3A tail 419-702

Depositor

Marvin Bentley

Article

Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

Insert

Kif 3A (KIF3A Human)

Use

Tags

Halo

Expression

Mammalian

Mutation

Promoter

Chicken Beta actin

Available since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pBa.Halo-KIF3B tail 391-747

Plasmid

#193929

Purpose

Mammalian expression of Halo-tagged Kif 3B tail 391-747

Depositor

Marvin Bentley

Article

Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

Insert

Kif 3B (Kif3b Mouse)

Use

Tags

Halo

Expression

Mammalian

Mutation

Promoter

Chicken Beta actin

Available since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF3A tail 419-702

Plasmid

#193923

Purpose

Mammalian expression of GFP-tagged Kif 3A tail 419-702

Depositor

Marvin Bentley

Article

Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

Insert

Kif 3A (KIF3A Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Chicken Beta actin

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pKM28

Plasmid

#154165

Purpose

Aedes aegypti Rel1A expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus

Depositor

Kevin Maringer

Article

Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

Insert

Rel1A

Use

Tags

FLAG

Expression

Insect

Mutation

Promoter

IE1 promoter and hr5 enhancer from Autographa cal…

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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KHBD00217

Plasmid

#34366

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG4158 (wor Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00171

Plasmid

#34324

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG10619 (tup Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pX330_sgACTA2

Plasmid

#63712

Purpose

Express sgACTA2 in mammalian cells.

Depositor

Stanley Qi

Article

Yu et al Cell Stem Cell. 2015 Feb 5;16(2):142-7.

Insert

ACTA2 (ACTA2 Mouse, Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_LaG17-mCherry-HOTag3

Plasmid

#190024

Purpose

Transiently expresses GFP-binding CluMPS (LaG17-mCh-HOTag3) in mammalian cells and lentiviral vector for constitutive expression.

Depositor

Lukasz Bugaj

Article

Mumford et al Cell Syst. 2024 Feb 21;15(2):166-179.e7.

Insert

LaG17 CluMPS

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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Flag p21 T145A

Plasmid

#16241

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2001 Mar . 3(3):245-52.

Insert

p21 (CDKN1A Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

T145A

Promoter

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
Flag p21 T145D

Plasmid

#16242

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2001 Mar . 3(3):245-52.

Insert

p21 (CDKN1A Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

T145D

Promoter

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pUB6-CDC34-HA (pRKB261)

Plasmid

#99145

Purpose

Mammalian expression construct to express HA-tagged CDC34

Depositor

Robert Bradley

Article

Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

Insert

CDC34 (CDC34 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

pTight

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pOmniBac zz TEV YBBR POT1

Plasmid

#185444

Purpose

Expresses human POT1 with a zz affinity tag, YBBR labeling site, and TEV cleavage site in insect cells

Depositor

Ahmet Yildiz

Article

Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.

Insert

POT1 (POT1 Human)

Use

Tags

YBBR and ZZ

Expression

Insect

Mutation

Promoter

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pBabe RFP1-Smad2 neo

Plasmid

#58491

Purpose

mammalian expression of mRFP1-tagged mouse Smad2

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

Smad2 (Smad2 Mouse)

Use

Tags

mRFP1

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 19, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-DEPTOR-WT

Plasmid

#159159

Purpose

Expresses DEPTOR in mammalian cells

Depositor

Michele Pagano

Article

Duan et al Mol Cell. 2011 Oct 21;44(2):317-24. doi:

Insert

DEPTOR (DEPTOR Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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myc-UVRAG

Plasmid

#86736

Purpose

N-terminal myc-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

Insert

human UVRAG (UVRAG Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pBI-CMV1-mir144[909 nt]~451-CMV2-mir1

Plasmid

#164681

Purpose

Express miR-144[909 nt]~451 and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-144[909 nt]~451 (MIR144 Human)
miR-1-1 (MIR1-1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-UVRAG

Plasmid

#86743

Purpose

N-terminal mCherry-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

Insert

human UVRAG (UVRAG Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pBI-CMV1-mir451~144-CMV2-mir1

Plasmid

#164666

Purpose

Express miR-451~144 and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-451~144 (MIR451A Human)
miR-1-1 (MIR1-1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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