We narrowed to 5,180 results for: pcDNA3.1+

1. NSP3C-Flag

   Plasmid

   #210349

   Purpose

   Expresses the C terminal of SARS-CoV-2 NSP3 fused with Flag in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   C terminal of SARS-CoV-2 NSP3 (ORF1ab )
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Nuc-mTurq2-Apollo-NADP+

   Plasmid

   #193302

   Purpose

   Expresses mTurq2-Apollo-NADP+ sensor localized to the nucleus

   Depositor

   Jonathan Rocheleau

   Article

   Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

   Insert

   G6PD (G6PD Human)
   

   Use

   Tags

   SV40 NLS and mTurqoise2

   Expression

   Mammalian

   Mutation

   S40A, R72Q, H201N, K205T

   Promoter

   T7

   Available since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA 3.1-nsp7-FLAG

   Plasmid

   #201027

   Purpose

   Expresses C-terminally FLAG tagged SARS-CoV-2 nsp7 in mammalian.

   Depositor

   Sharon Rozovsky

   Article

   Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

   Insert

   non-structural protein 7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
   

   Use

   Tags

   8xHis and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. CoV2-N-G204R

   Plasmid

   #177953

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (G204R). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (G204R) (N )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G204R

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. CaMKIV-DCTK75E

   Plasmid

   #126423

   Purpose

   A kinase-dead mutant of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)

   Depositor

   Douglas Black

   Article

   Xie et al Nature. 2001 Apr 19;410(6831):936-9. doi

   Insert

   calcium/calmodulin-dependent protein kinase IV kinase-dead mutant (CAMK4 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed lysine 75 to glutamic acid

   Promoter

   Available since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-LYRa11-RBD-8h

   Plasmid

   #161822

   Purpose

   Mammalian expression plasmid for RBD of bat coronavirus isolate LYRa11

   Depositor

   Erik Procko

   Article

   Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

   Insert

   Receptor-binding domain (RBD) of spike protein S
   

   Use

   Tags

   GSG linker and 8his tag and HA leader sequence

   Expression

   Mammalian

   Mutation

   Human codon optimized RBD (a.a. T324-D522)

   Promoter

   CMV

   Available since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. mEmerald(luminal)-canxTM

   Plasmid

   #186961

   Purpose

   Expresses mEmerald connected with the N-terminus of Calnexin transmembrane domain.

   Depositor

   Ke Xu

   Article

   Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

   Insert

   CANX (CANX Human)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Only express calnexin (482-end) with ER signal pe…

   Promoter

   CMV Promoter

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. FLAG HDAC5 S259A/S498A

   Plasmid

   #32216

   Depositor

   Reuben Shaw

   Article

   Mihaylova et al Cell. 2011 May 13;145(4):607-21.

   Insert

   HDAC5 (HDAC5 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Ser259Ala, Ser498Ala

   Promoter

   CMV

   Available since

   Oct. 20, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pcDNATM3.1/V5-His TOPO-PKCi-K274W

   Plasmid

   #55690

   Purpose

   expresses dominant negative human PKCi tagged with V5

   Depositor

   Pedro Salas

   Article

   Wald et al J Cell Sci. 2008 Mar 1;121(Pt 5):644-54.

   Insert

   Homo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   A120E and K274W

   Promoter

   CMV

   Available since

   Aug. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pAB07 (CK2beta)

    Plasmid

    #27091

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Insert

    CK2beta (CSNK2B Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3-RsSHC014-RBD-8h

    Plasmid

    #161826

    Purpose

    Mammalian expression plasmid for RBD of bat coronavirus isolate RsSHC014

    Depositor

    Erik Procko

    Article

    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

    Insert

    Receptor-binding domain (RBD) of spike protein S
    

    Use

    Tags

    GSG linker and 8his tag and HA leader sequence

    Expression

    Mammalian

    Mutation

    Human codon optimized RBD (a.a. T321-D519)

    Promoter

    CMV

    Available since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3 proHB-EGF NC

    Plasmid

    #11605

    Depositor

    Rosalyn Adam

    Article

    Kim et al Cancer Res. 2005 Sep 15. 65(18):8242-9.

    Insert

    HB-EGF (HBEGF Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Non-cleavable HB-EGF: Pro149 to Thr160 replaced w…

    Promoter

    Available since

    April 26, 2006

    Availability

    Academic Institutions and Nonprofits only

13. ALFA-bArr1

    Plasmid

    #201495

    Purpose

    Expression of the beta-arrestin1 containing a N-terminal ALFA tag.

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    ALFA-bArr1 (ARRB1 Human)
    

    Use

    Tags

    ALFA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-HCMVgO-sfGFP (Merlin)

    Plasmid

    #136452

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein O (Merlin strain) fused to superfolder GFP

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    glycoprotein O (UL74 Human betaherpesvirus 5)
    

    Use

    Tags

    Gly/Ser-rich linker (GGSGGSGSGG) (includes BamHI …

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. V5-bArr1

    Plasmid

    #201491

    Purpose

    Expression of the beta-arrestin1 containing a N-terminal V5 tag.

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    V5-bArr1 (ARRB1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. FLAG-GAS41

    Plasmid

    #15428

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

    Insert

    GAS41 (Yeats4 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

17. Opto-GPR3

    Plasmid

    #106000

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR3; Opto-GPR3; A2)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR3 (GPR3 Human, Bovine)
    

    Use

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-Rs7327-RBD-8h

    Plasmid

    #161823

    Purpose

    Mammalian expression plasmid for RBD of bat coronavirus isolate Rs7327

    Depositor

    Erik Procko

    Article

    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

    Insert

    Receptor-binding domain (RBD) of spike protein S
    

    Use

    Tags

    GSG linker and 8his tag and HA leader sequence

    Expression

    Mammalian

    Mutation

    Human codon optimized RBD (a.a. T321-D519)

    Promoter

    CMV

    Available since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pC1-IMS-oROS-HT

    Plasmid

    #216419

    Purpose

    Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the inter-membrane-space of mitochondria.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.02.06.579232

    Insert

    oROS-HT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNATM3.1/V5-His TOPO-PKCi-A120E

    Plasmid

    #55687

    Purpose

    expresses constitutively active human PKCi tagged with V5

    Depositor

    Pedro Salas

    Article

    Wald et al J Cell Sci. 2008 Mar 1;121(Pt 5):644-54.

    Insert

    Homo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    A120E

    Promoter

    CMV

    Available since

    July 28, 2014

    Availability

    Academic Institutions and Nonprofits only

21. pTE3646

    Plasmid

    #137714

    Purpose

    Expresses LbCas12a RVRR variant in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

    Insert

    LbCas12a-RVRR
    

    Use

    Tags

    3xHA, NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    G532R, K538V, Y542R, K595R

    Promoter

    CMV

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. nSaCas9 I744 miniABEMax V82G

    Plasmid

    #135379

    Purpose

    Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (I744 )

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSaCas9 I744 miniABEMax V82G
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    (V82G)

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3-Rs4084-RBD-8h

    Plasmid

    #161825

    Purpose

    Mammalian expression plasmid for RBD of bat coronavirus isolate Rs4084

    Depositor

    Erik Procko

    Article

    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

    Insert

    Receptor-binding domain (RBD) of spike protein S
    

    Use

    Tags

    GSG linker and 8his tag and HA leader sequence

    Expression

    Mammalian

    Mutation

    Human codon optimized RBD (a.a. T321-D519)

    Promoter

    CMV

    Available since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3-Rs4231-RBD-8h

    Plasmid

    #161824

    Purpose

    Mammalian expression plasmid for RBD of bat coronavirus isolate Rs4231

    Depositor

    Erik Procko

    Article

    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

    Insert

    Receptor-binding domain (RBD) of spike protein S
    

    Use

    Tags

    GSG linker and 8his tag and HA leader sequence

    Expression

    Mammalian

    Mutation

    Human codon optimized RBD (a.a. T320-D518)

    Promoter

    CMV

    Available since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CoV2-N-D3L

    Plasmid

    #177945

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (D3L). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (D3L) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D3L

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3-HCMVgH-8his (TB40/E)

    Plasmid

    #136445

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein H (TB40/E strain) extracellular domain with 8his purification tag

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    glycoprotein H (UL75 Human betaherpesvirus 5)
    

    Use

    Tags

    Short GGSG linker and 8his tag

    Expression

    Mammalian

    Mutation

    Extracellular domain (a.a. 1-717). Codon-optimize…

    Promoter

    CMV

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. FLAG-MED29

    Plasmid

    #15409

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

    Insert

    MED29 (MED29 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

28. CoV2-N-A119S

    Plasmid

    #177948

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (A119S). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (A119S) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A119S

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PCDNA3-FlipYFP(TEV cleavage seq) T2A mCherry

    Plasmid

    #124493

    Purpose

    Expresses FlipYFP (TEV cleavage sequence) and T2A mCherry

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipYFP(TEV cleavage seq) T2A mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3-HCMVgO-8his (TB40/E)

    Plasmid

    #136453

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein O (TB40/E strain) with an 8his tag

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    envelope glycoprotein O (UL74 Human betaherpesvirus 5)
    

    Use

    Tags

    Linker and 8his tag (GGSGHHHHHHHH, includes BamHI…

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge