We narrowed to 5,111 results for: codon optimized

1. UMG-SP-1_pET29b

   Plasmid

   #215433

   Purpose

   Expression construct for UMG-SP-1 gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   UMG-SP-1
   

   Expression

   Bacterial

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. UMG-SP-2_pET29b

   Plasmid

   #215434

   Purpose

   Expression construct for UMG-SP-2 gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   UMG-SP-2
   

   Expression

   Bacterial

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti6.3-efSaCas9-BlastR

   Plasmid

   #220971

   Purpose

   Expresses human codon-optimized efSaCas9

   Depositor

   Hyongbum Kim

   Article

   Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

   Insert

   efSaCas9
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBEAST_J23101-bphC

   Plasmid

   #128137

   Purpose

   The cell-free adapted backbone, pBEAST, expressing bphC (the enzyme converting biphenyl-2,3-diol to 2-hydroxy-6-oxo-6-phenylhexa-2,4-) under control of the constitutive promoter J23101, and RBS B0032

   Depositor

   Jean-Loup Faulon

   Article

   Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

   Insert

   bphC
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   J23101

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJC30X-BS(X)

   Plasmid

   #58958

   Purpose

   Encodes synthetic mitochondrial gene GFPm-3 in place of COX3 coding sequence. Flanked by mtDNA sequences allowing integration into rho+ mtDNA after transformation.

   Depositor

   Thomas Fox

   Article

   Cohen et al Mitochondrion. 2001 Aug;1(2):181-9.

   Insert

   Enhanced fluorescence variant of GFP, in S. cerevisiae mitochondrial genetic code
   

   Tags

   First 8 codons of COX3

   Mutation

   GFP modified as described: CORMACK, B. P., R. H. …

   Promoter

   n/a

   Available Since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pscALPS SIV-MND2 vpx

   Plasmid

   #115811

   Purpose

   Encodes codon optimized 3xFLAG-SIVMND2 Vpx (AY159322) and puromycin resistance protein

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   SIVMND2 Vpx (AY159322)
   

   Use

   Lentiviral

   Tags

   3x-FLAG

   Promoter

   SFFV

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. NylB_pET29b

   Plasmid

   #215414

   Purpose

   Expression construct for NylB gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   NylB
   

   Expression

   Bacterial

   Available Since

   May 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSCL.028

   Plasmid

   #184965

   Purpose

   Test effect of extending -Eco1 a1/a2 in yeast

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1: RT and ncRNA(extended), a1/a2 length: 27
   

   Expression

   Yeast

   Mutation

   human codon optimized RT, a1/a2 length extended t…

   Promoter

   Gal7

   Available Since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKMD106h - intein-Cas9(C574)

   Plasmid

   #64191

   Purpose

   Expresses intein-Cas9(C574) in mammalian cells

   Depositor

   David Liu

   Article

   Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi

   Insert

   mammalian codon-optimized S. pyogenes intein-Cas9(C574) - NLS - 3x FLAG
   

   Use

   CRISPR

   Tags

   NLS - 3x FLAG (C terminal on insert)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. MSP2283

    Plasmid

    #70702

    Purpose

    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Inserts

    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. ALIX(P801G)-Strep

    Plasmid

    #180024

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses ALIX(1-868, P801G)-Strep. P801G mutation facilitates expression in E. coli.

    Depositor

    Lalit Deshmukh

    Article

    Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

    Insert

    ALIX(1-868, P801G)-Strep (PDCD6IP Human)
    

    Tags

    Strep tag (WSHPQFEK)

    Expression

    Bacterial

    Mutation

    P801G

    Available Since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. TOPO rtTA3-2A-Cre

    Plasmid

    #68448

    Purpose

    TOPO construct expressing rtTA3-2A-Cre for generation of GMAP-compatible gene

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    rtTA3-2A-Cre
    

    Use

    Gmap

    Expression

    Bacterial

    Mutation

    Codon optimized for expression in mice

    Promoter

    Plac

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-HCMVgL-FLAG (Merlin)

    Plasmid

    #136450

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein L (Merlin strain) with FLAG tag

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    glycoprotein L (UL115 Human betaherpesvirus 5)
    

    Tags

    Short SGGSG linker and FLAG tag

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. Tt-NylC_pET28a

    Plasmid

    #215431

    Purpose

    Expression construct for Tt-NylC gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    Tt-NylC
    

    Expression

    Bacterial

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pC1300_pUB10_pco-vCASphi_E9t_MCS_version2

    Plasmid

    #197961

    Purpose

    T-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter.

    Depositor

    Steven Jacobsen

    Article

    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

    Insert

    pco-vCasphi-2
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    pUBQ10

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pC1300_pUB10_pco-nCASphi_E9t_MCS_version2

    Plasmid

    #197962

    Purpose

    T-DNA binary vector to express pco-nCasphi driven by UBQ10 gene promoter.

    Depositor

    Steven Jacobsen

    Article

    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

    Insert

    pco-nCasphi-2
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    pUBQ10

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1 FLAG- SIV-MAC251-vpx-Q76A

    Plasmid

    #115843

    Purpose

    Encodes codon optimized FLAG tagged SIVMAC251-Vpx-Q76A mutant

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SIVMAC251-Vpx-Q76A
    

    Tags

    3x-FLAG

    Expression

    Mammalian

    Mutation

    Q76A

    Promoter

    CMV

    Available Since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVRc03_1198

    Plasmid

    #49730

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    AS03_1198
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pLux

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCVL.SFFV.Kozak.HA.NLS.Y2Ani.IRES.BFP

    Plasmid

    #45578

    Purpose

    Expresses Y2 variant of I-Ani I nuclease in mammalian cells with BFP tracker

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Insert

    coY2 Ani
    

    Use

    Lentiviral

    Tags

    HA, IRES BFP, and NLS

    Expression

    Mammalian

    Mutation

    co indicates codon optimization for mammalian exp…

    Promoter

    SFFV

    Available Since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pscALPS-SIV-RCM vpx

    Plasmid

    #115812

    Purpose

    Encodes codon optimized 3xFLAG-SIVRCM Vpx (AF349680) and puromycin resistance protein

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SIVRCM Vpx (AF349680)
    

    Use

    Lentiviral

    Tags

    3x-FLAG

    Promoter

    SFFV

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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