We narrowed to 4,276 results for: gca

1. CMV-NLS-R-GECO

   Plasmid

   #32462

   Purpose

   Mammalian expression of nucleus-targeting red fluorescent genetically encoded Ca2+ indicator for optical imaging

   Depositor

   Robert Campbell

   Article

   Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

   Insert

   R-GECO1.0
   

   Use

   Tags

   NLS sequence DPKKKRKV

   Expression

   Mammalian

   Mutation

   Substitutions relative to the mApple-derived anal…

   Promoter

   CMV

   Available since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLQ7558 pHR (hU6-crB2M-EFS-PuroR-WPRE)

   Plasmid

   #214881

   Purpose

   Lentiviral vector encoding RfxCas13d targeting B2M guide

   Depositor

   Stanley Qi

   Article

   Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

   Insert

   hU6-crB2M-EFS-PuroR-WPRE
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   April 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSCIP-hCD69-TdTomato

   Plasmid

   #154095

   Purpose

   Self-Cutting and Integrating CRISPR backbone targeting human CD69 promoter with TdTomato reporter transgene

   Depositor

   Scott McComb

   Article

   Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

   Inserts

   [5HA]-P2A-tdTomato-[3HA]
   hCD69 targeting sgRNA - AGCTCTTTGCATCCGGAGAG
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Cas9-GFP_sg_mTfeb

   Plasmid

   #79006

   Purpose

   Expresses WT Cas9 and GFP, along with a sgRNA to mouse Tfeb.

   Depositor

   Reuben Shaw

   Article

   Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

   Insert

   sgTfeb (Tfeb Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. shMEK1/2 puro

   Plasmid

   #72567

   Purpose

   Hairpin targeting MEK1 and MEK2 (murine).

   Depositor

   Kevin Janes

   Article

   Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

   Insert

   MEK1/2 (Map2k1, Map2k2 Mouse)
   

   Use

   Lentiviral

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. sg_Brd9_i5-ipUSEPR-TR657

   Plasmid

   #228922

   Purpose

   Knockdown of Brd9 in mammalian cells

   Depositor

   Sika Zheng

   Article

   Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

   Insert

   sg_Brd9_i5 (Brd9 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. sg_Brd9_i2-ipUSEPR-TR657

   Plasmid

   #228921

   Purpose

   Knockdown of Brd9 in mammalian cells

   Depositor

   Sika Zheng

   Article

   Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

   Insert

   sg_Brd9_i2 (Brd9 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.1P CISD1_1

   Plasmid

   #160773

   Purpose

   Suppress CISD1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   shCISD1_1
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCR1163

   Plasmid

   #111099

   Purpose

   Expresses FANCD2_+_10068200.23-P1P2 sgRNA in pCR1068

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

   Insert

   FANCD2 gRNA (FANCD2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-Puro-sgNUAK2

    Plasmid

    #138693

    Purpose

    Expresses a human NUAK2-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgNUAK2 human (NUAK2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO.1-puro-irf3-shrna1

    Plasmid

    #127648

    Purpose

    Knock-down of human IRF3

    Depositor

    Nicolas Manel

    Article

    Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

    Insert

    IRF3 shRNA (IRF3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. RNASEL gRNA (BRDN0001149212)

    Plasmid

    #77051

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RNASEL

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RNASEL (RNASEL Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. shRNA_circHUWE1_1

    Plasmid

    #215236

    Purpose

    Supression of shcircHUWE1(19,20)_1 expression

    Depositor

    Sefi Rosenbluh

    Article

    Liu et al Nat Cell Biol. 2024 Aug;26(8):1359-1372.

    Insert

    circHUWE1 shRNA 1 (HUWE1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. PX458_KMT2B_2

    Plasmid

    #101075

    Purpose

    Encodes gRNA for 3' target of human KMT2B

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against KMT2B (KMT2B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. PX459-TP53-exon5

    Plasmid

    #217456

    Purpose

    For TP53 knockout targeting exon 5 of TP53

    Depositor

    John Diffley

    Article

    Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

    Insert

    CRISPR Cas9 guide for TP53 (TP53 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. GFP-itis pBE-nt

    Plasmid

    #195343

    Purpose

    Base editor plasmid expressing ABE8e-NG-Cas9n under Theophylline Riboswitch and constituitively expressed nonsense non-targeting gRNA

    Depositor

    Alexis Komor

    Article

    Vasquez et al CRISPR J. 2023 Apr 20. doi: 10.1089/cris

    Inserts

    ecTadA(8e)-SpCas9-NG
    gRNA gtgcacgacgccgtatgcga
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    SpCas9n-NG: D10A, L1111R, D1135V, G1218R, E1219F,…

    Promoter

    Lac and ProC

    Available since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330-PITCh-VIM

    Plasmid

    #227301

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of VIM for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of VIM (VIM Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-FLEX-SaCas9-U6-sgGabrg2

    Plasmid

    #124857

    Purpose

    Mutagenesis of Gabrg2

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gabrg2 (Gabrg2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. LENTICRISPR-UPP1_sgRNA2

    Plasmid

    #201635

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    UPP1 (UPP1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. POLR2A-N CRISPR pX330

    Plasmid

    #124495

    Purpose

    A CRISPR plasmid for targeting the N-terminus coding region of human POLR2A

    Depositor

    Masato Kanemaki

    Article

    Nagashima et al J Cell Biol. 2019 Mar 1. pii: jcb.201811

    Insert

    POLR2A targeting CRISPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6 promoter

    Available since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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