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pcDNA4/HisMaxB-YAP2-1st WW mutant

Plasmid

#18998

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

His

Expression

Mammalian

Mutation

Tryptophan 199 changed to Alanine, and Proline 2…

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA4/HisMaxB-YAP2-2nd WW mutant

Plasmid

#18999

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

His

Expression

Mammalian

Mutation

Tryptophan 258 changed to Alanine, and Proline 2…

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pCMV5 N539

Plasmid

#25972

Depositor

Rob Lewis

Article

Joneson et al J Biol Chem. 1998 Mar 27. 273(13):7743-8

Insert

Kinase suppressor of Ras 1 (Ksr1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Deleted amino acids 540-873

Available Since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
pCMV5 C540

Plasmid

#25971

Depositor

Rob Lewis

Article

Joneson et al J Biol Chem. 1998 Mar 27. 273(13):7743-8

Insert

Kinase suppressor of Ras 1 (Ksr1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Deletes amino acids 1-539

Available Since

Aug. 24, 2010

Availability

Academic Institutions and Nonprofits only
pET28-His6-SUMO-Yes-SH2-AviTag

Plasmid

#214208

Purpose

Bacterial expression of SH2 domain of the Yes kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections

Depositor

Neel Shah

Article

Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi

Insert

Yes kinase SH2 domain (YES1 Human)

Tags

6xHis, AviTag, and SUMO

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-DIO-ExRai-AKAR2-T/A

Plasmid

#171847

Purpose

Cre-dependent, synapsin promoter-driven expression of the negative control phosphomutant ExRai-AKAR2 T/A PKA biosensor in neurons.

Depositor

Richard Huganir

Article

Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

Insert

ExRai-AKAR2 T/A

Use

AAV

Expression

Mammalian

Mutation

T6A phospho-deficient mutation

Promoter

hSyn1

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti-TO-HA-Δ37 PPM1H-mito

Plasmid

#208375

Purpose

Lentiviral expression of human Δ37-PPM1H with C-terminal mito signal

Depositor

Suzanne Pfeffer

Article

Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

HA-delta37 PPM1H-mito (PPM1H Human)

Use

Lentiviral

Expression

Mammalian

Mutation

delta37

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti-TO-Δ37 PPM1H -PACT-HA

Plasmid

#208373

Purpose

Lentiviral expression of human Δ37-PPM1H with C-terminal HA tag

Depositor

Suzanne Pfeffer

Article

Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

delta37 PPM1H -PACT-HA (PPM1H Human)

Use

Lentiviral

Expression

Mammalian

Mutation

delta37

Available Since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti-TO-Δ37 PPM1H-TMEM115-HA

Plasmid

#208374

Purpose

Lentiviral expression of human Δ37-PPM1H with C-terminal TMEM115-HA

Depositor

Suzanne Pfeffer

Article

Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

delta37 PPM1H-TMEM115-HA (PPM1H Human)

Use

Lentiviral

Expression

Mammalian

Mutation

delta37

Available Since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-NEDD4L_FL-CS

Plasmid

#187713

Purpose

Express the catalytically inactive GST-tagged NEDD4L FL in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L FL with C874 mutated to a serine (NEDD4L Human)

Tags

GST-HRV 3C site

Expression

Bacterial

Mutation

Cys874 mutated to a serine

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 myc-Rab10-mito

Plasmid

#208367

Purpose

Mammalian expression of myc-tagged human Rab10 with C-terminal mito signal

Depositor

Suzanne Pfeffer

Article

Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

myc-Rab10-mito (RAB10 Human)

Expression

Mammalian

Mutation

wildtype

Available Since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

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GFP Cav2.2 D122A pcDNA3

Plasmid

#206068

Purpose

expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and a D122A mutation that disrupts the interaction with ?2?-1

Depositor

Annette Dolphin

Article

Page et al J Neurosci. 2004 Jun 9;24(23):5400-9. do

Insert

cacna1b (CACNA1B Rabbit)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pT22iU66CriT(#387)

Plasmid

#184061

Purpose

cyclofen-inducible CRE activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Sinha et al Zebrafish. 2010 Jun;7(2):199-204. doi: 1

Insert

CRE-ERT2

Use

Zebrafish transgenesis (blue eyes)

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Zf-Ubi

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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eSpCas9(1.1)-ABL1-gRNA1-exon4

Plasmid

#163322

Purpose

eCas9 ABL1 gRNA 1 (GGGGGACACACCATAGACAG), targeting exon 4 within the protein kinase domain of both ABL1 isoforms 1a and 1b.

Depositor

Val Brunton

Article

Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

Insert

ABL1 gRNA 1_Exon4

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only
eSpCas9(1.1)-ABL1-gRNA2-exon4

Plasmid

#163323

Purpose

eCas9 ABL1 gRNA 2 (GAAGAAATACAGCCTGACGG), targeting exon 4 within the protein kinase domain of both ABL1 isoforms 1a and 1b.

Depositor

Val Brunton

Article

Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

Insert

ABL1 gRNA 2_Exon4

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only
pSUMO His6 SUMO SnRK2.3 (19-361)

Plasmid

#177846

Purpose

Bacterial Expression of SnRK2.3

Depositor

Karsten Melcher

Article

Ng et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

Insert

SNF1-related protein kinase 2.3 (SNRK2.3 Mustard Weed)

Tags

6xHis-SUMO

Expression

Bacterial

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pMAL c2x Tral N

Plasmid

#113009

Purpose

For bacterial expression of MBP fusion of N terminal region of Drosophila Tral

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

N terminus of tral (tral Fly)

Expression

Bacterial

Mutation

amino acids 1-355

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pMAL c2x Tral C

Plasmid

#113008

Purpose

For bacterial expression of MBP fusion of C terminal region of Drosophila Tral

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

C terminus of tral (tral Fly)

Expression

Bacterial

Mutation

amino acids 356-652

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pMAL c2x Tral C 15A

Plasmid

#113007

Purpose

For bacterial expression of MBP fusion of C terminal region of Drosophila Tral phosphomutant (15A)

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

C terminus of tral (tral Fly)

Expression

Bacterial

Mutation

PNG phosphorylation sites mutated to A

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pEf1a-superDn29-dCas9-P2A-GFP

Plasmid

#247156

Purpose

Mammalian expression of a human codon optimized engineered superDn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

superDn29-dCas9-P2A-GFP

Tags

SV40 NLS

Expression

Mammalian

Mutation

M6I/E70G/A224P/G227V/I233K/K248R/I303K/Q332K/N341…

Promoter

Ef1a

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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