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pHMWGFPR

Plasmid

#64396

Purpose

High molecular weight glutenin gene promoter driving GFP expression

Depositor

Claudia Vickers

Article

Vickers et al Plant Mol Biol. 2006 Sep;62(1-2):195-214

Insert

High molecular weight glutenin gene promoter

Tags

GFP

Expression

Plant

Promoter

High molecular weight glutenin gene promoter

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only
GST-PRMT7 LDIG69AAAA

Plasmid

#61560

Purpose

Hs GST-PRMT7 LDIG(69-72)AAAA AdoMet binding deficient mutant

Depositor

Steven Clarke

Article

Feng et al J Biol Chem. 2014 Nov 21;289(47):32604-1

Insert

PRMT7 (PRMT7 Human)

Tags

GST

Expression

Bacterial

Mutation

LDIG 69-72 AAAA

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
CBP-HAT-SP65

Plasmid

#21088

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

Insert

CBP (Crebbp Mouse)

Use

In vitro tnt

Tags

Flag

Mutation

HAT domain aa 1172-1621

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pLV CDHblast Rev(V16D)-EGFP-V5

Plasmid

#175165

Purpose

Lentiviral expression of Rev(V16D)-EGFP-V5

Depositor

Larry Gerace

Article

Hammond et al J Mol Biol. 2018 Feb 16;430(4):537-553.

Insert

rev

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

V16D

Available Since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) ZKSCAN1 MCS-GFP 5' Half Only

Plasmid

#69907

Purpose

Expresses a circular RNA containing the 5' half of GFP in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

GFP, ZKSCAN1 (ZKSCAN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pFTK086

Plasmid

#171358

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

P-ANtRNA[Arg21]-sgRNA-dummy-Esp3I, Pol-III sgRNA-plug-in transcription unit

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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HOXA9 (murine) HIS-tag pET

Plasmid

#8492

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

Insert

HoxA9 (Hoxa9 Mouse)

Tags

His and T7

Expression

Bacterial

Mutation

BamHI/BalI fusion into amino acid #2 of HOXA9; ma…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pFA6a-3YFP-kanMX6

Plasmid

#105149

Purpose

Yeast gene targeting

Depositor

David Kovar, Thomas Pollard, Jian-Qiu Wu

Article

Wu et al J Cell Biol. 2006 Jul 31;174(3):391-402.

Insert

3YFP-kanMX6

Tags

3YFP

Expression

Bacterial and Yeast

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCMB-TMEg

Plasmid

#61209

Purpose

Fluorescent reporter for plasma membrane and trunk peri-arbuscular membrane MtBCPsp-GFP-BCP expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtBCPsp-GFP-BCP

Tags

EGFP

Expression

Plant

Promoter

MtBCP1

Available Since

April 10, 2015

Availability

Academic Institutions and Nonprofits only

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CBP-CH3-SP65

Plasmid

#21092

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

Insert

CBP (Crebbp Mouse)

Use

In vitro tnt

Mutation

CH3 domain aa 1625-1877

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pML2(−)WF

Plasmid

#108337

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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HOXC6-pCT

Plasmid

#21000

Depositor

Corey Largman

Article

Lawrence et al Cell Growth Differ. 1993 Aug . 4(8):665-

Insert

HOXC6 (HOXC6 Human)

Expression

Mammalian

Mutation

Long 5' UTR, approx 480 nt

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
GST-PRMT9 G260E mutant

Plasmid

#67610

Purpose

bacterial expression of GST-PRMT9 G260E Double E loop mutant

Depositor

Steven Clarke

Article

Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

Insert

PRMT9 (PRMT9 Human)

Tags

GST

Expression

Bacterial

Mutation

G260E double E loop mutant

Promoter

tac

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
GST-PRMT9 D258G mutant

Plasmid

#67609

Purpose

bacterial expression of GST-PRMT9 D258G Double E loop mutant

Depositor

Steven Clarke

Article

Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

Insert

PRMT9 (PRMT9 Human)

Tags

GST

Expression

Bacterial

Mutation

D258G double E loop mutant

Promoter

tac

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only
pT2K-CAGGS-U6-sgRNA-M9-IRES-CFP

Plasmid

#114731

Purpose

sgRNA targeting a sequence upstream of the initiator ATG of the cellular Myc gene

Depositor

Martine Roussel

Article

Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

Insert

sgRNA M9

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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CBP-Bromo-HAT-SP65

Plasmid

#21090

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

Insert

CBP (Crebbp Mouse)

Use

In vitro tnt

Tags

Flag

Mutation

Bromo+HAT domains aa 1107-1621

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pML2(+)BR

Plasmid

#108336

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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pML2(+)WF

Plasmid

#108333

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pML2(+)BF

Plasmid

#108334

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pML2(+)WR

Plasmid

#108335

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge