We narrowed to 5,876 results for: nls

1. AP76_pU.CAG.Cas9-D10A-K848A.rBGpA

   Plasmid

   #199253

   Purpose

   Expression construct encoding the high specificity SpCas9-KA-D10A nickase

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

   Insert

   High specificity S. pyogenes Cas9-D10A-KA nickase
   

   Use

   CRISPR

   Tags

   3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A K848A

   Promoter

   CAG promoter

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAT15543_dABE-SuperFi-Cas9

   Plasmid

   #184373

   Purpose

   Mammalian expression of nuclease inactive SuperFi-Cas9 ABE7 base editor

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

   Insert

   dABE-SuperFi-Cas9
   

   Use

   CRISPR

   Tags

   FLAG, SV40 NLS, and nucleoplasmin NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…

   Promoter

   EF-1α core promoter

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAT15545_dABE8e-SuperFi-Cas9

   Plasmid

   #184375

   Purpose

   Mammalian expression of nuclease inactive SuperFi-Cas9 ABE8e base editor

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

   Insert

   dABE8e-SuperFi-Cas9
   

   Use

   CRISPR

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…

   Promoter

   EF-1α core promoter

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDIV495

   Plasmid

   #177702

   Purpose

   Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii

   Depositor

   Uffe Mortensen

   Article

   Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

   Inserts

   Cas9
   Nat
   sgRNA Targeting ADE2 locus in D.hansenii: AGCTAaGCAGATTAAtGCAT
   

   Use

   CRISPR

   Tags

   SV40

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   RNR2p (Debaryomyces hansenii ), SRC1p (Debaryomyc…

   Available since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. deltaVP1 only

   Plasmid

   #166675

   Purpose

   Yeast integrative plasmid for expressing deltaVP1, an NLS deletion mutant of Murine polyomavirus VP1 (GAL1 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

   Insert

   Murine polyomavirus deltaVP1 (NLS deletion mutant)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHA841 - hrp-1p::hrp-1HsLCWT

   Plasmid

   #139198

   Purpose

   Expresses hrp-1p::hrp-1HsLCWT::hrp-1 3'UTR

   Depositor

   Anne Hart

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   hrp-1 (hrp-1 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   last exon replaced with correspoding human sequen…

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHA842 - hrp-1p::hrp-1HsLCD290V

   Plasmid

   #139199

   Purpose

   Expresses hrp-1p::hrp-1HsLCD290V::hrp-1 3'UTR

   Depositor

   Anne Hart

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   hrp-1 (hrp-1 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   D290V, last exon replaced with correspoding human…

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHA847 - mec-4p::hrp-1mScarlet

   Plasmid

   #139201

   Purpose

   Expresses mScarlet tagged hrp-1 in mec-4 neurons

   Depositor

   Anne Hart

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   hrp-1 (hrp-1 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   mScarlet added between RRMs and LC

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHA848 - mec-4p::hrp-1HsLCWTmScarlet

   Plasmid

   #139202

   Purpose

   Expresses mScarlet tagged hrp-1HsLCWT in mec-4 neurons

   Depositor

   Anne Hart

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   hrp-1 (hrp-1 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   mScarlet added between RRMs and LC, last exon rep…

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHA849 - mec-4p::hrp-1HsLCD290VmScarlet

    Plasmid

    #139203

    Purpose

    Expresses mScarlet tagged hrp-1HsLCD290V in mec-4 neurons

    Depositor

    Anne Hart

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hrp-1 (hrp-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    mScarlet added between RRMs and LC, D290V, last e…

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHA845 - hrp-1p::hrp-1HsLCWTmScarlet

    Plasmid

    #139205

    Purpose

    Expresses mScarlet tagged hrp-1HsLCWT

    Depositor

    Anne Hart

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hrp-1 (hrp-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    mScarlet added between RRMs and LC, last exon rep…

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHA846 - hrp-1p::hrp-1HsLCD290VmScarlet

    Plasmid

    #139206

    Purpose

    Expresses mScarlet tagged hrp-1HsLCD290V

    Depositor

    Anne Hart

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hrp-1 (hrp-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    mScarlet added between RRMs and LC, D290V, last e…

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. hnRNPF_PLD

    Plasmid

    #139113

    Purpose

    express prion like domain of hnRNPF

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hnRNPF (HNRNPF Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    365-415

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. MTK3a_025

    Plasmid

    #123777

    Purpose

    Encodes dspCAS9 tagged with NLS as a Type 3a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    spdCAS9 (with NLS)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. RNAPII_CTD27-52_K5S_S1966C

    Plasmid

    #98683

    Purpose

    Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with K5S and S1966C

    Depositor

    Nicolas Fawzi

    Article

    Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

    Insert

    RNA polymerase II C-terminal domain (POLR2A Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    K1838S, K1859S, K1866S, K1873S, K1887S, S1966C

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. STEME-1

    Plasmid

    #138137

    Purpose

    Targeted simultaneous C-to-T and A-to-G in rice protoplasts

    Depositor

    Caixia Gao

    Insert

    APOBEC3A-wtTadA-TadA7.10-nCas9-UGI-NLS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. A3A-PBE-NG

    Plasmid

    #138141

    Purpose

    Targeted C-to-T in rice protoplasts

    Depositor

    Caixia Gao

    Insert

    APOBEC3A-nCas9-NG-UGI-NLS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAdx-CMV-YFP

    Plasmid

    #73348

    Purpose

    Express YFP-NLS under CMV promoter

    Depositor

    Kazuhiro Oka

    Insert

    CMV-YFP
    

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. STEME-2

    Plasmid

    #138138

    Purpose

    Targeted simultaneous C-to-T and A-to-G in rice protoplasts

    Depositor

    Caixia Gao

    Insert

    wtTadA-TadA7.10-APOBEC3A-nCas9-UGI-NLS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. SpRY-YE1-BE4max

    Plasmid

    #198554

    Purpose

    Expresses human codon-optimized SpRY-YE1-BE4max and blasticidin resistance: EFS promoter-SpRY-YE1-BE4max-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpRY-YE1-BE4max
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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