We narrowed to 13,971 results for: EGFP

Showing: 7001 - 7050 of 13971 results

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pLV-Hygro-EF1A-Af1521(K35E/Y145R)-EGFP

Plasmid

#196235

Purpose

EGFP fused to the C-terminus of Af1521(K35E/Y145R) macrodomain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

C-terminal EGFP tagged Af1521(K35E/Y145R)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

K35E and Y145R

Promoter

EF1A

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pET-29_GSS-6xHis-SUMO-VidC-EGFP-FLAG

Plasmid

#241009

Purpose

VidC-EGFP construct that can be purified via His-tag and undergo N-terminal conjugation using the VEPL system.

Depositor

Cole DeForest

Article

Gharios et al Bioconjug Chem. 2024 Jun 19;35(6):750-75

Insert

GSS-6xHis-SUMO-VidC-EGFP-FLAG

Tags

FLAG-tag, His-tag, and SUMO-tag

Expression

Bacterial

Promoter

T7

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-Gluc-NHP.RMA-IRES-EGFP

Plasmid

#227887

Purpose

Expresses Gluc-NHP.RMA and EGFP in the presence of Cre recombinase. Double-floxed Gluc-NHP.RMA and EGFP for monitoring Cre-expressing neuronal populations.

Depositor

Jerzy Szablowski

Article

Lee et al bioRxiv 2025.06.01.657212; doi: 10.1101/

Inserts

Gaussia luciferase fused to macaque Fc
IRES-EGFP

Use

AAV, Cre/Lox, and Luciferase

Tags

Gluc and IgG1 Fc

Expression

Mammalian

Available Since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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pL(EF1a-phi-AQP1-FKBP12DD-IRES-EGFP-WPRE)

Plasmid

#236279

Purpose

Lentiviral vector that can express hAqp1 with fkbp12 degron in mammalian cells. EF1a promoter. EGFP marker.

Depositor

Arnab Mukherjee

Article

Yun et al Chem Sci. 2024 Jun 4;15(28):11108-11121.

Insert

human aquaporin 1 (AQP1 Human)

Use

Lentiviral

Tags

Flag and fkbp12

Expression

Mammalian

Promoter

EF1a

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pL(CMVtight-phi-AQP1-FKBP12DD-IRES-EGFP-WPRE)

Plasmid

#236278

Purpose

Lentiviral vector that can express doxycycline-inducible hAqp1 with fkbp12 degron in Tet ON mammalian cells. CMVtight promoter. EGFP marker.

Depositor

Arnab Mukherjee

Article

Yun et al Chem Sci. 2024 Jun 4;15(28):11108-11121.

Insert

human aquaporin 1 (AQP1 Human)

Use

Lentiviral

Tags

Flag and fkbp12

Expression

Mammalian

Promoter

CMVtight

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV[FLEX]-CAG-EGFP-pri-miR-Ctrl

Plasmid

#235158

Purpose

Expresses EGFP and a control primary transcript in a Cre recombinase-dependent manner

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

EGFP/primary transcript with control siRNA

Use

AAV

Promoter

CAG

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV[FLEX]-CAG-EGFP-miR-206-sponge

Plasmid

#235154

Purpose

Expresses a EGFP-based sponge for miR-206 in a Cre recombinase-dependent manner

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

EGFP/miR-206 sponge with 12x binding sites

Use

AAV

Promoter

CAG

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV[FLEX]-CAG-EGFP-miR-133-sponge

Plasmid

#235155

Purpose

Expresses a EGFP-based sponge for miR-133 in a Cre recombinase-dependent manner

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

EGFP/miR-133 sponge with 12x binding sites

Use

AAV

Promoter

CAG

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV[FLEX]-CAG-EGFP-pri-miR-137

Plasmid

#235159

Purpose

Expresses EGFP and miR-137 primary transcript in a Cre recombinase-dependent manner

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

EGFP/pri-miR-137 (Mir137 Mouse)

Use

AAV

Promoter

CAG

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-TadCBEd-SpCas9(MRKQKC)-P2A-EGFP (AHK574)

Plasmid

#223134

Purpose

pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with MRKQKC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized TadA-CDd-SpCas9(MRKQKC)-2xUGI-P2A-EGFP

Use

CRISPR

Tags

2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

Expression

Mammalian

Mutation

TadA-CDd mutations; nSpCas9(D10A/MRKQKC)=D1135M, …

Promoter

CMV and T7

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-TadCBEd-SpCas9(MRKSKC)-P2A-EGFP (AHK446)

Plasmid

#223135

Purpose

pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with MRKSKC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized TadA-CDd-SpCas9(MRKSKC)-2xUGI-P2A-EGFP

Use

CRISPR

Tags

2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

Expression

Mammalian

Mutation

TadA-CDd mutations; nSpCas9(D10A/MRKSKC)=D1135M, …

Promoter

CMV and T7

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-TadCBEd-SpCas9(KWRQLC)-P2A-EGFP (BKS1026)

Plasmid

#223126

Purpose

pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with KWRQLC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized TadA-CDd-SpCas9(KWRQLC)-2xUGI-P2A-EGFP

Use

CRISPR

Tags

2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

Expression

Mammalian

Mutation

TadA-CDd mutations; nSpCas9(D10A/KWRQLC)=D1135K, …

Promoter

CMV and T7

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-TadCBEd-SpCas9(LWRYEK)-P2A-EGFP (BKS1022)

Plasmid

#223128

Purpose

pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with LWRYEK amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized TadA-CDd-SpCas9(LWRYEK)-2xUGI-P2A-EGFP

Use

CRISPR

Tags

2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

Expression

Mammalian

Mutation

TadA-CDd mutations; nSpCas9(D10A/LWRYEK)=D1135L, …

Promoter

CMV and T7

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-TadCBEd-SpCas9(MKRCMV)-P2A-EGFP (BKS1032)

Plasmid

#223129

Purpose

pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with MKRCMV amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized TadA-CDd-SpCas9(MKRCMV)-2xUGI-P2A-EGFP

Use

CRISPR

Tags

2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

Expression

Mammalian

Mutation

TadA-CDd mutations; nSpCas9(D10A/MKRCMV)=D1135M, …

Promoter

CMV and T7

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-TadCBEd-SpCas9(MKRCKS)-P2A-EGFP (RAS3445)

Plasmid

#223130

Purpose

pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with MKRCKS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized TadA-CDd-SpCas9(MKRCKS)-2xUGI-P2A-EGFP

Use

CRISPR

Tags

2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

Expression

Mammalian

Mutation

TadA-CDd mutations; nSpCas9(D10A/MKRCKS)=D1135M, …

Promoter

CMV and T7

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-TadCBEd-SpCas9(ARGIMR)-P2A-EGFP (BKS1036)

Plasmid

#223132

Purpose

pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with ARGIMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized TadA-CDd-SpCas9(ARGIMR)-2xUGI-P2A-EGFP

Use

CRISPR

Tags

2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

Expression

Mammalian

Mutation

TadA-CDd mutations; nSpCas9(D10A/ARGIMR)=D1135A, …

Promoter

CMV and T7

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-TadCBEd-SpCas9(MRKCRS)-P2A-EGFP (AHK545)

Plasmid

#223133

Purpose

pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with MRKCRS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized TadA-CDd-SpCas9(MRKCRS)-2xUGI-P2A-EGFP

Use

CRISPR

Tags

2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

Expression

Mammalian

Mutation

TadA-CDd mutations; nSpCas9(D10A/MRKCRS)=D1135M, …

Promoter

CMV and T7

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-ABE8e-SpCas9(LWQYQH)-P2A-EGFP (AHK504)

Plasmid

#223119

Purpose

pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with LWQYQH amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized TadA8e-SpCas9(LWQYQH)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-P2A-EGFP and BPNLS-TadA8e

Expression

Mammalian

Mutation

TadA8e mutations; nSpCas9(D10A/LWQYQH)=D1135L, S1…

Promoter

CMV and T7

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-ABE8e-SpCas9(MWKYQS)-P2A-EGFP (AHK505)

Plasmid

#223120

Purpose

pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized TadA8e-SpCas9(MWKYQS)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-P2A-EGFP and BPNLS-TadA8e

Expression

Mammalian

Mutation

TadA8e mutations; nSpCas9(D10A/MWKYQS)=D1135M, S1…

Promoter

CMV and T7

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-ABE8e-SpCas9(MWKYQA)-P2A-EGFP (AHK503)

Plasmid

#223122

Purpose

pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MWKYQA amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized TadA8e-SpCas9(MWKYQA)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-P2A-EGFP and BPNLS-TadA8e

Expression

Mammalian

Mutation

TadA8e mutations; nSpCas9(D10A/MWKYQA)=D1135M, S1…

Promoter

CMV and T7

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-ABE8e-SpCas9(ARGIMR)-P2A-EGFP (AHK507)

Plasmid

#223118

Purpose

pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with ARGIMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized TadA8e-SpCas9(ARGIMR)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-P2A-EGFP and BPNLS-TadA8e

Expression

Mammalian

Mutation

TadA8e mutations; nSpCas9(D10A/ARGIMR)=D1135A, S1…

Promoter

CMV and T7

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-ABE8e-SpCas9(KWRQLC)-P2A-EGFP (RAS3467)

Plasmid

#223112

Purpose

pCMV and pT7 Human expression plasmid for ABE8e SpCas9 enzyme with KWRQLC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized TadA8e-SpCas9(KWRQLC)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-P2A-EGFP and BPNLS-TadA8e

Expression

Mammalian

Mutation

TadA8e mutations; nSpCas9(D10A/KWRQLC)=D1135K, S1…

Promoter

CMV and T7

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-ABE8e-SpCas9(MRKCRS)-P2A-EGFP (BKS1034)

Plasmid

#223117

Purpose

pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MRKCRS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized TadA8e-SpCas9(MRKCRS)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-P2A-EGFP and BPNLS-TadA8e

Expression

Mammalian

Mutation

TadA8e mutations; nSpCas9(D10A/MRKCRS)=D1135M, S1…

Promoter

CMV and T7

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSynI-EGFP-WPRE-hGH poly(A) (Alex_02)

Plasmid

#71651

Purpose

AAV expression of EGFP in human and mouse neurons

Depositor

Mirjana Maletić-Savatić

Insert

EGFP

Use

AAV

Expression

Mammalian

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
KL158 - pBlueScript II SK-T2A-eGFP-SV40pA Podxl donor

Plasmid

#232936

Purpose

Donor plasmid for knock-in of T2A-eGFP-SV40pA at the mouse Podxl locus. Used with PX459-sgRNA047_Podxl sgRNA.

Depositor

Danny Reinberg

Article

Lucero et al bioRxiv, March 19, 2025

Insert

T2A-eGFP-SV40pA with Podxl homology arms

Use

Mouse Targeting

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX4T1_GST-EGFP-ATG13_IDR_191-517aa_(M196-H214 11xA)

Plasmid

#223779

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

ATG13 IDR (ATG13 Human)

Tags

GST-EGFP

Expression

Bacterial

Mutation

expresses aa 191-517, with M196A S197A R199A G202…

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX4T1_GST-EGFP-ATG13_IDR_delta206-230 (191-205_231-517aa)

Plasmid

#223787

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

ATG13 IDR (ATG13 Human)

Tags

GST-EGFP

Expression

Bacterial

Mutation

expresses aa 191-517, with 206-230 deleted

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-FKBP-mEGFP-WIPI2 IDR (364-425aa)

Plasmid

#223758

Purpose

Stable expression and inducible recruitment of protein in mammalian cell culture

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

WIPI2 IDR (WIPI2 Human)

Use

Lentiviral

Tags

FKBP-mEGFP

Mutation

aa 364-425 only

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pETDuet1_6xHis-TEV-EGFP-FIP200-CTR(1429-1591aa)

Plasmid

#223724

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

FIP200-CTR (RB1CC1 Human)

Tags

6xHis-TEV-EGFP

Expression

Bacterial

Mutation

aa 1429-1591 only

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.U6-sasgRNA(SapI)_Ple155-CI-EGFP-W3SL_BbsI(GGA)

Plasmid

#231367

Purpose

Ple155-driven EGFP, also encoding U6-driven sgRNA cassette, with SapI sites to clone crRNA sequence

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

EGFP

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Const.3 EGFP-P2A-dTomato-T2A-TagBFP-PS9

Plasmid

#231907

Purpose

For packaging EGFP, dTomato and TagBFP mRNA into SARS-CoV-2 virus-like particles (VLPs)

Depositor

Sriram Neelamegham

Article

Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

EGFP-P2A-dTomato-T2A-TagBFP

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Const.5 EGFP-P2A-dTomato-IRES-Luc-PS9

Plasmid

#231909

Purpose

For packaging EGFP, dTomato and firefly luciferase mRNA into SARS-CoV-2 virus-like particles (VLPs)

Depositor

Sriram Neelamegham

Article

Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

EGFP-P2A-dTomato-IRES-Luc

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pME nls EGFP WPRE bGH pA (JDW 488)

Plasmid

#224540

Purpose

A Gateway compatible middle entry clone containing a 3x-NLS-EGFP for labeling the nucleus

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

nls-EGFP-WPRE-bGH-pA

Use

Gateway cloning

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO-puro SNAP-CD86-eGFP-FKBP(f36v)

Plasmid

#200283

Purpose

Membrane protein fusion with extracellular SNAPtag and intracellular FKBP(f36v) dimerization domain and GFP reporter under Tetracycline-inducible promoter

Depositor

Kristina Ganzinger

Article

Niederauer et al Nat Commun. 2023 Jul 19;14(1):4345. doi:

Insert

SNAPtag-eGFP-CD86tm-eGFP-FKBP(f36v)

Use

Lentiviral

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pX552-EF1a-DIO Chronos-eGFP(with gRNA scaffold)

Plasmid

#199582

Purpose

Expresses Chronos-eGFP in a Cre-dependent manner

Depositor

Bryan Copits

Article

Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

Insert

Chronos eGFP

Use

AAV, CRISPR, and Cre/Lox

Tags

GFP

Expression

Mammalian

Promoter

EF1a intron A

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGB3_alpha1_LIR1-35S-eGFP-T35S-SIR-LIR2 (GB4312)

Plasmid

#225435

Purpose

BeYDV LIR1 + TU 35S:eGFP:T35S + BeYDV SIR-LIR2.

Depositor

Diego Orzaez

Article

Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

Insert

LIR1:35S:eGFP:T35S-SIR:LIR2

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHIV-NAT-T2A-hCD52-eGFP-NFATC2IP-SPR

Plasmid

#221447

Purpose

Lentiviral mammalian expression vector of eGFP-tagged NFATC2IP ORF that is fused with S-phase restricted (SPR) degron at its C-terminus

Depositor

Daniel Durocher

Article

Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

Insert

NFATC2IP (NFATC2IP Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

SPR degron: hGEM (1-110) fragment that is fused t…

Promoter

CMV

Available Since

July 3, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pOTTC1642 - pAAV EF1a EGFP-KASH with mTACR1 gRNAs

Plasmid

#195018

Purpose

An adeno-associated viral vector expressing eGFP-KASH and two guides targeting mouse TACR1

Depositor

Christopher Richie

Article

Belilos et al bioRxiv [Preprint]. 2023 Oct 9:2023.02.0

Inserts

CCGTATAGGCGGCTGCCCAA
EGFP-KASH
TTCCGTGGTGGGCAACGTAG

Use

AAV

Tags

KASH domain of Nesprin2

Promoter

EF1a, hU6, and mU6

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pNES-SET-EMD-EGFP (C-NES-SET-EMD)

Plasmid

#217770

Purpose

Fluorescent reporter for ceramide (putative, mammalian expression)

Depositor

Paula Nunes-Hasler

Article

Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

Insert

SET (SET Human)

Tags

EGFP

Expression

Mammalian

Mutation

EMD domain (aa 70-226) plus nuclear export sequen…

Promoter

CMV

Available Since

June 12, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNES-PRKCZ-C20-EGFP (C-NES-PRKCZ-C20)

Plasmid

#217763

Purpose

Fluorescent reporter for ceramide (putative, mammalian expression)

Depositor

Paula Nunes-Hasler

Article

Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

Insert

Protein kinase C zeta (PRKCZ Human)

Tags

EGFP

Expression

Mammalian

Mutation

NES (ALQKKLEELELDEAPVAT) plus C20 domain (aa 405-…

Promoter

CMV

Available Since

June 11, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pGS58_6xHis-EGFP-CENP-T[1-242/3D (T11, T27, T85)] - I3-01

Plasmid

#211890

Purpose

Bacterial expression of CENP-T[1-242/3D]-I3-01

Depositor

Iain Cheeseman

Insert

CENP-T[1-242/3D]-I3-01 (CENPT Synthetic, Human)

Expression

Bacterial

Mutation

T11D, T27D, T85D

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pLV UBC OSBP(PH)-APEX2-3xFLAG-IRES-eGFP

Plasmid

#218637

Purpose

Proximity biotinylation control of OSBP(PH domain)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

OSBP (OSBP Human)

Use

Lentiviral

Tags

APEX2-3xFLAG

Expression

Mammalian

Mutation

PH domain only

Promoter

UBC

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-Nier1s-3NLS-P2A-EGFP-3NLS

Plasmid

#217478

Purpose

Mammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1s

Depositor

Peng Zou

Article

Lin et al Cell Rep Methods. 2023 Jul 27;3(8):10054

Insert

Nier1s

Tags

3xNLS, P2A-EGFP-3xNLS

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-Nier1b-3NLS-P2A-EGFP-3NLS

Plasmid

#217479

Purpose

Mammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1b

Depositor

Peng Zou

Article

Lin et al Cell Rep Methods. 2023 Jul 27;3(8):10054

Insert

Nier1b

Tags

3xNLS, P2A-EGFP-3xNLS

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pTY186 EGFP-T2A-ALFA-ORF6 SARS-CoV-2

Plasmid

#204976

Purpose

Co-express EGFP and SARS-CoV-2 ORF6 N-terminally labeled with ALFA tag

Depositor

Tim Mitchison

Article

Yoo et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

Insert

Open Reading Frame 6 (ORF6 SARS-CoV-2)

Tags

ALFA

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pGS275_6xHis-EGFP-CENP-T[1-242/3D (T11, T27, T85)]

Plasmid

#211891

Purpose

Bacterial expression of CENP-T[1-242/3D]

Depositor

Iain Cheeseman

Insert

CENP-T[1-242/3D] (CENPT Human)

Expression

Bacterial

Mutation

T11D, T27D, T85D

Available Since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-RPL3-191~307-mEGFP(A206K)-TwStrep

Plasmid

#208626

Purpose

for E. coli expression of truncated human RPL3 (ENST00000216146), aa191~307, fused with mEGFP(A206K) and Twin Strep tag

Depositor

Quanfu Ma

Insert

human RPL3 (ENST00000216146) aa 191~307 (RPL3 Human)

Tags

6xHis, Twin Strep, and mEGFP(A206K)

Expression

Bacterial

Mutation

only aa 191~307, other residues not included

Promoter

T7

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-RPL3-299~398-mEGFP(A206K)-TwStrep

Plasmid

#208627

Purpose

for E. coli expression of truncated human RPL3 (ENST00000216146), aa299~398, fused with mEGFP(A206K) and Twin Strep tag

Depositor

Quanfu Ma

Insert

human RPL3 (ENST00000216146) aa 299~398 (RPL3 Human)

Tags

6xHis, Twin Strep, and mEGFP(A206K)

Expression

Bacterial

Mutation

only aa 299~398, other residues not included

Promoter

T7

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-RPL3-212~285-mEGFP(A206K)-TwStrep

Plasmid

#208629

Purpose

for E. coli expression of truncated human RPL3 (ENST00000216146), aa212~285, fused with mEGFP(A206K) and Twin Strep tag

Depositor

Quanfu Ma

Insert

human RPL3 (ENST00000216146) aa 212~285 (RPL3 Human)

Tags

6xHis, Twin Strep, and mEGFP(A206K)

Expression

Bacterial

Mutation

only aa 212~285, other residues not included

Promoter

T7

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pFH2.99_PB-EPN1-MCP_I-T2A-eGFP-PGK-Blast

Plasmid

#205544

Purpose

Express EPN1-MCP-I-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN1-MCP-I

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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