We narrowed to 75,856 results for: Rest

1. pOpen-9N7polA

   Plasmid

   #165500

   Purpose

   Thermophilic DNA polymerase; genetically engineered to have a decreased 3'-5' proofreading exonuclease activity

   Depositor

   Jennifer Molloy, FreeGenes Project, Drew Endy

   Insert

   9 degrees N-7 DNA polymerase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 26, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pcDNA3-IL-2signal-6xMyc

   Plasmid

   #128033

   Purpose

   Mammalian expression vector with N terminal IL-2 signal peptide and 6xMyc tag

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Use

   Tags

   6xMyc and IL-2 signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUC118

   Plasmid

   #50009

   Purpose

   pUC cloning vector

   Depositor

   Joachim Messing

   Article

   Vieira et al Methods Enzymol. 1987;153:3-11.

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. L4417

   Plasmid

   #1649

   Depositor

   Andrew Fire

   Type

   Empty backbone

   Use

   RNAi

   Tags

   T7p, gfpC, gfpS65S, gfpN, T7p, lacZN, OriF1>&g…

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTJK299

   Plasmid

   #138000

   Purpose

   SIX1 expression. Vector also expresses GFP under control of hUBC promoter

   Depositor

   Tami Kingsbury

   Insert

   SIX1 (SIX1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   May 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. 1478 pcDNA3 flag HA 14-3-3 beta

   Plasmid

   #8999

   Depositor

   William Sellers

   Insert

   14-3-3 beta (YWHAB Human)
   

   Use

   Tags

   HA and flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3-GFP-MMP7

   Plasmid

   #11989

   Depositor

   Steven Johnson

   Insert

   MMP7 (MMP7 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 25, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pOpen-THEAQlig

   Plasmid

   #165535

   Purpose

   Thermostable ligase for incorporation of phosphorylated oligonucleotides during PCR and Ligase Chain Reaction

   Depositor

   Jennifer Molloy, FreeGenes Project, Drew Endy

   Insert

   Taq DNA Ligase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 5, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pBABE-neo largeTgenomic

   Plasmid

   #10891

   Depositor

   William Hahn

   Insert

   large T and small T antigen (SV40gp6 simian virus)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

10. pQUASp-mCD8mCherry

    Plasmid

    #46164

    Purpose

    Expression of murine CD8mCherry in insects

    Depositor

    Christopher Potter

    Insert

    mCD8mCherry
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti6MQBI163 GLUT2

    Plasmid

    #194138

    Purpose

    Expresses GLUT2 protein in mammalian cells

    Depositor

    Jun Liu

    Insert

    SLC2A2 (SLC2A2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPD95_77

    Plasmid

    #1495

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Use

    Tags

    IVSA, rf2204, gfpN, IVSR, gfpS65C, IVSS, IVST, gf…

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV6-p85alpha-Flag

    Plasmid

    #1399

    Depositor

    Lewis Cantley

    Insert

    PI3K regulatory subunit p85alpha (Pik3r1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AV0-BWS-2.0-mC-G-CL3B

    Plasmid

    #124975

    Purpose

    evaluate autophagy

    Depositor

    Kazuhiro Oka

    Insert

    mCherry-GFP-LC3B
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1-alpha

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ADH1pro_GAL4_SIR4

    Plasmid

    #138621

    Purpose

    Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement

    Depositor

    Rob Mitra

    Insert

    GAL4 (GAL4 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pESC-NAT-LACap

    Plasmid

    #80763

    Purpose

    Expresses truncated L-A capsid for curing satellite virus

    Depositor

    John McCusker

    Insert

    L-A cDNA (L-A Cap)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PGK1

    Available since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pOpen-UDG

    Plasmid

    #165531

    Purpose

    Uracil DNA glycosylase catalyzes the hydrolysis of the N-glycosidic bond from deoxyuridine to release uracil.

    Depositor

    Jennifer Molloy, FreeGenes Project, Drew Endy

    Insert

    UDG
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. FUGW-EYFP

    Plasmid

    #128074

    Purpose

    3rd gen lentiviral plasmid with hUbC-driven EYFP

    Depositor

    Oskar Laur

    Insert

    EYFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    no

    Promoter

    Available since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCSIIbleo-iRFP670-P2A-EGFP

    Plasmid

    #198060

    Purpose

    The lentiviral vector for iRFP670 and EGFP

    Depositor

    Michiyuki Matsuda

    Insert

    iRFP670
    

    Use

    Lentiviral

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOpen-Bstpol

    Plasmid

    #165543

    Purpose

    Full length DNA polymerase from Bacillus stearothermophilus.

    Depositor

    Jennifer Molloy, FreeGenes Project, Drew Endy

    Insert

    Bst DNA Polymerase, Full Length
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. pPGS-hSLUG.fl.flag

    Plasmid

    #25696

    Depositor

    Eric Fearon

    Insert

    SLUG (SNAI2 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    July 9, 2010

    Availability

    Academic Institutions and Nonprofits only

22. pET16b-Rnl2(1-249)K227Q

    Plasmid

    #14072

    Depositor

    Thomas Tuschl

    Insert

    Rnl2(1-249)
    

    Use

    Tags

    His (10x)

    Expression

    Bacterial

    Mutation

    changed Lys227 to Glutamine Gly112 is actually C…

    Promoter

    Available since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

23. CMV-hEAAT4

    Plasmid

    #32816

    Depositor

    Susan Amara

    Insert

    hEAAT4 (SLC1A6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

24. pOpen-TthpolA

    Plasmid

    #165533

    Purpose

    DNA Polymerase whose activity is resistant to prolonged incubations at high temperatures (95 degrees C).

    Depositor

    Jennifer Molloy, FreeGenes Project, Drew Endy

    Insert

    Tth DNA polymerase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pcDNA3-TLR6-CFP

    Plasmid

    #13021

    Depositor

    Doug Golenbock

    Insert

    Toll-Like Receptor 6 (TLR6 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pT3TS

    Plasmid

    #31830

    Depositor

    Stephen Ekker

    Article

    Hyatt et al Methods Cell Biol. 1999;59:117-26.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Lac

    Available since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET28GST-LIC

    Plasmid

    #26101

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Tags

    6XHis, GST, and Thrombin CLeavage Site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 20, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pUPD2 GFP-CT (GB2238)

    Plasmid

    #160592

    Purpose

    GFP for C-terminal fusions.

    Depositor

    Diego Orzaez

    Insert

    GFP-CT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. XE10 Xwnt-8 CS2+

    Plasmid

    #16865

    Depositor

    Randall Moon

    Insert

    Xwnt-8 (wnt8a.L Frog)
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 13, 2008

    Availability

    Academic Institutions and Nonprofits only

30. pcDNA3-Flag-Siah1 Ring Mutant

    Plasmid

    #122040

    Purpose

    express Siah1 RM

    Depositor

    Ze'ev Ronai

    Insert

    Siah1 RM
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ring mutant H58A and C62A

    Promoter

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSV40_HA-mPACT

    Plasmid

    #120537

    Purpose

    protein expression

    Depositor

    Petr Svoboda

    Insert

    PACT (Prkra Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pSty11

    Plasmid

    #12401

    Depositor

    Titia de Lange

    Article

    de Lange et al EMBO J. 1992 Feb . 11(2):717-24.

    Insert

    Sty11
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

33. pET His6 Mocr TEV LIC cloning vector (2O-T)

    Plasmid

    #29710

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6-Mocr-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBNP-NFAT-luciferase

    Plasmid

    #24956

    Depositor

    Toren Finkel

    Insert

    BNP promoter (NPPB Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET His6 ProteinG TEV LIC cloning vector (2P-T)

    Plasmid

    #29713

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6-ProteinG-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCRT7 NT Topo arabinose

    Plasmid

    #73446

    Purpose

    Used for arabinose-induced expression of N-term GST/C-term 6xHis tagged peptides

    Depositor

    Jesse Rinehart

    Type

    Empty backbone

    Use

    Tags

    GST (N-terminal), 6xHis (C-terminal)

    Expression

    Bacterial

    Mutation

    Promoter

    pBad

    Available since

    March 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pEBG-hOSR1-Flag

    Plasmid

    #185362

    Purpose

    For mammalian expression of human GST-OSR1-Flag

    Depositor

    Ayaz Najafov

    Insert

    OXSR1 (OXSR1 Human)
    

    Use

    Tags

    Flag and GST

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pIRES-H3.3WT-BFP

    Plasmid

    #209442

    Purpose

    Expression plasmid expressing H3.3 Wt histone in tandem with BFP

    Depositor

    Maria Castro

    Insert

    H3.3-WT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. BII-tdT-inv-GFP

    Plasmid

    #133393

    Purpose

    Piggybac vector for bidirectional promoter reporter assay

    Depositor

    Blair Madison

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    No promoter

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. 864 pSG5L HA NLS PTEN

    Plasmid

    #10933

    Depositor

    William Sellers

    Insert

    PTEN (PTEN Human)
    

    Use

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. EGFP-PKCZ

    Plasmid

    #110512

    Purpose

    Fluorescent PKCZ

    Depositor

    Bernardo Mainou

    Insert

    PKCZ (PRKCZ Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pWPI-myogenin

    Plasmid

    #79073

    Purpose

    Lentiviral plasmid that constitutively expresses human myogenin

    Depositor

    Jennifer Lei

    Insert

    human myogenin (MYOG Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1 alpha

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. mCherry-PKCZ

    Plasmid

    #110511

    Purpose

    Fluorescent PKCZ

    Depositor

    Bernardo Mainou

    Insert

    PKCZ (PRKCZ Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pKG333

    Plasmid

    #69751

    Purpose

    Retroviral expression of Histone H3

    Depositor

    Iain Cheeseman

    Insert

    Histone H3
    

    Use

    Retroviral

    Tags

    eGFP-TEV-S

    Expression

    Mammalian

    Mutation

    e.coli optimized sequence

    Promoter

    Available since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. 863 pSG5L HA NES PTEN

    Plasmid

    #10932

    Depositor

    William Sellers

    Insert

    PTEN (PTEN Human)
    

    Use

    Tags

    HA and NES

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. XE238 hVangl2/Strabismus pCS2P+

    Plasmid

    #16992

    Depositor

    Randall Moon

    Insert

    Vangl2/Strabismus (VANGL2 Human)
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 13, 2008

    Availability

    Academic Institutions and Nonprofits only

47. pHIE096 CMV Bxb1

    Plasmid

    #139252

    Purpose

    Encodes a Bxb1 recombinase for Landing Pad integration.

    Depositor

    Joshua Leonard

    Insert

    Bxb1 Recombinase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pCMV WS Full Myc (Wrn cDNA)

    Plasmid

    #19273

    Depositor

    Philip Leder

    Insert

    mouse Wrn cDNA (Wrn Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pTRE-T2-∆mIR-IRES-Puromycin

    Plasmid

    #105168

    Purpose

    Mammalian expression vector for expressing tandem cDNAs

    Depositor

    Michael E. Wright

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet/CMV

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. BS II KS+ loxP-GFP_ZEO_loxP

    Plasmid

    #128064

    Purpose

    has loxp-GFP-Zeo-Loxp cassette

    Depositor

    Oskar Laur

    Insert

    loxp-GFP-Zeo-Loxp
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    no

    Promoter

    Available since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge