We narrowed to 10,316 results for: plasmids 101

1. R620-M11-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6

   Plasmid

   #170195

   Purpose

   Mammalian expression of SARS-CoV-2 soluble spike trimer protein

   Depositor

   Dominic Esposito

   Article

   Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

   Insert

   SARS-CoV-2 S (spike) (S SARS-CoV-2)
   

   Use

   Tags

   His6

   Expression

   Mammalian

   Mutation

   Promoter

   CMV51

   Available Since

   Nov. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. K84M kinase-dead Nuak1-3xflag

   Plasmid

   #97219

   Purpose

   Plasmid expressing kinase-dead mutant Nuak1

   Depositor

   Huda Zoghbi

   Article

   Lasagna-Reeves et al Neuron. 2016 Oct 19;92(2):407-418. doi:

   Insert

   Nuak1 (NUAK1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Bacterial and Mammalian

   Mutation

   Kinase-dead K84M

   Promoter

   T7

   Available Since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. R619-M64-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2 D614G

   Plasmid

   #166011

   Purpose

   mammalian expression of SARS-CoV-2 soluble spike trimer protein (D614G mutant)

   Depositor

   Dominic Esposito

   Article

   Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

   Insert

   SARS-CoV-2 S (spike) (S )
   

   Use

   Tags

   3C-His8-Strep2x2

   Expression

   Mammalian

   Mutation

   D614G

   Promoter

   CMV51

   Available Since

   May 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCOLADuet-1_nsp10 (SARS-CoV-2)

   Plasmid

   #169158

   Purpose

   To express SARS-CoV-2 nsp10 in E. coli

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

   Insert

   nsp10 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Methionine

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T7

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS D614 soluble Spike

   Plasmid

   #164652

   Purpose

   Expresses SARS-CoV-2 soluble Spike protein

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   SARS-CoV-2 soluble spike (S SARS-CoV-2)
   

   Use

   Tags

   HIS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

6. pTwist-SARS-CoV-2 Spike-deltaC18 BA.2

   Plasmid

   #212487

   Purpose

   Encodes SARS-CoV-2 variant BA.2 Spike for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike BA.2 (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   See Depositor Comments.

   Promoter

   CAG

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL3-noSV40-humanCOL2A1enhancer

   Plasmid

   #173961

   Purpose

   Firefly luciferase enhancer reporter plasmid.

   Depositor

   Joanna Wysocka

   Article

   Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

   Insert

   Enhancer region from COL2A1 gene
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   None

   Promoter

   SV40 promoter

   Available Since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-EF1a-FLuc-WPRE-HGHpA

   Plasmid

   #87951

   Purpose

   Recombinant single-stranded AAV transfer vector expressing Firefly Luciferase under the EF1a promoter

   Depositor

   Mark Kay

   Article

   Paulk et al Mol Ther. 2017 Sep 25. pii: S1525-0016(1

   Insert

   Firefly Luciferase
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTwist-SARS-CoV-2 Spike-deltaC18 HK.3

   Plasmid

   #212572

   Purpose

   Encodes SARS-CoV-2 spike for HK.3 pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike HK.3 (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   See Depositor Comments.

   Promoter

   CAG

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPB-TRE-V5-TurboID-T2A-SARS-CoV2-NSP7-bGH polyA > mPGK-BlastR-SV40 polyA

    Plasmid

    #218914

    Purpose

    PiggyBac transposition

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    V5-TurboID-T2A-SARS-CoV2-NSP7/BlastR (ORF1ab )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    2363G>C and 4330T>C

    Promoter

    Available Since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pαH-S-HRV3C

    Plasmid

    #164567

    Purpose

    SARS-CoV-2 Spike protein with furin site mutated to HRV3C site (S-HRV3C variant)

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

    Insert

    Spike - Furin site changed for HRV3C site
    

    Use

    Tags

    2X Strep-Tag II and 8X His tag

    Expression

    Mammalian

    Mutation

    682-685 (furin site) replaced with HRV3C site (LE…

    Promoter

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1 SARS-CoV-2 S D614G-trunc

    Plasmid

    #158077

    Purpose

    Expresses SARS-CoV-2 spike protein with a D614G truncated mutation

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D614G

    Promoter

    Available Since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL3-noSV40-humanEC1.45

    Plasmid

    #173946

    Purpose

    Firefly luciferase enhancer reporter plasmid.

    Depositor

    Joanna Wysocka

    Article

    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

    Insert

    SOX9 enhancer cluster EC1.45
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    None

    Promoter

    SV40 promoter

    Available Since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIG-747_CD19-28z_CAR_TFAP4

    Plasmid

    #207489

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    TFAP4, CD19-28z_CAR (TFAP4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTwist-SARS-CoV-2 Δ18 K417N+E484K+N501Y

    Plasmid

    #169469

    Purpose

    Encodes SARS-CoV-2 K417N+E484K+N501Y Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV-2 K417N+E484K+N501Y Spike truncated to remove 18 amino acids (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1 SARS-CoV-2 S D614-trunc

    Plasmid

    #158076

    Purpose

    Expresses SARS-CoV-2 spike protein with a truncation

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTwist-SARS-CoV-2 Δ18 B.1.351v2/wtRBD

    Plasmid

    #169467

    Purpose

    Encodes SARS-CoV-2 B.1.351v2/wtRBD Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV-2 B.1.351v2/wtRBD Spike truncated to remove 18 amino acids (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBIG1a_3xFlag-6His-nsp15 (SARS-CoV-2)

    Plasmid

    #169166

    Purpose

    Baculoviral transfer vector for the expression of SARS-CoV-2 nsp15 in insect cells

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2465-2479

    Insert

    3xFlag-6His-nsp15 (ORF1ab Synthetic, SARS-CoV-2)
    

    Use

    Tags

    3xFlag-6His

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTwist-SARS-CoV-2 Spike-deltaC18 E484Q (CAA)

    Plasmid

    #212423

    Purpose

    Encodes SARS-CoV-2 spike E484Q for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike E484Q (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E484Q

    Promoter

    CAG

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTwist-SARS-CoV-2 Spike-deltaC18 L981F (TTC)

    Plasmid

    #212486

    Purpose

    Encodes SARS-CoV-2 spike L981F for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike L981F (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L981F

    Promoter

    CAG

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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