We narrowed to 3,452 results for: ttl

1. pXD70Tet(DadRmt7)-N-3xFLAG-DadR(linker)

   Plasmid

   #191643

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (TetR), N-3xFLAG-dadR(linker region shuffled) complementation

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   DadR
   

   Tags

   3xFLAG

   Expression

   Bacterial

   Mutation

   Linker region sequence shuffled

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pXD70Tet(DadH)-Pdadh-DadhABC(A2)

   Plasmid

   #191644

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (TetR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   Dopamine dehydroxylase
   

   Expression

   Bacterial

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPQ265N2

   Plasmid

   #174001

   Purpose

   Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-SpRY-UNG for C-G base editing

   Depositor

   Yiping Qi

   Article

   Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

   Insert

   rAPOBEC1-zCas9(D10A)-SpRY-UNG
   

   Use

   CRISPR

   Tags

   3X FLAG, NLS

   Expression

   Plant

   Mutation

   In zCas9(D10A)-SpRY: D10A, A61R, L1111R, D1135L, …

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ265O2

   Plasmid

   #174002

   Purpose

   Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-SpRY-rXRCC1 for C-G base editing

   Depositor

   Yiping Qi

   Article

   Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

   Insert

   rAPOBEC1-zCas9(D10A)-SpRY-rXRCC1
   

   Use

   CRISPR

   Tags

   3X FLAG, NLS

   Expression

   Plant

   Mutation

   In zCas9(D10A)-SpRY: D10A, A61R, L1111R, D1135L, …

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYPQ265L2

   Plasmid

   #174000

   Purpose

   Gateway entry clone (attL1 & attR5) UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY for C-G base editing

   Depositor

   Yiping Qi

   Article

   Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

   Insert

   UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY
   

   Use

   CRISPR

   Tags

   3X FLAG, NLS

   Expression

   Plant

   Mutation

   In rAPOBEC1: R33A; in zCas9(D10A)-SpRY: D10A, A61…

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRecomb-loxP-mCherry-4Gcloningsite-lox2272-66

   Plasmid

   #168787

   Purpose

   Donor vector for recombinase mediated integration in filamentous fungi. Is a shuttle vector for optional in vivo cloning in yeast, and contains four inducible promoters or sites for whole BGC cloning.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al ACS Synth Biol. 2022 Feb 16. doi: 10.102

   Type

   Empty backbone

   Use

   Cre/Lox and Synthetic Biology; Expression in fila…

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221 CARD8 ZUC

   Plasmid

   #169964

   Purpose

   pDONR221 for Gateway shuttling. Contains the CARD8 FIIND and CARD (L162M-L537, no stop codon)

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Gateway cloning vector

   Available Since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. E1_pENTR_L1-L4_NI_G1333-DddtoxA-C

   Plasmid

   #171724

   Purpose

   ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

   Depositor

   Shin-ichi Arimura

   Article

   Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

   Insert

   C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
   

   Use

   Multisite gateway entry vector

   Available Since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. E1_pENTR_L1-L4_NN_G1333-DddtoxA-C

   Plasmid

   #171728

   Purpose

   ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

   Depositor

   Shin-ichi Arimura

   Article

   Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

   Insert

   C- and N-termini of TALEN, half of a cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
   

   Use

   Multisite gateway entry vector

   Available Since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. E3_pENTR_L3-L2_NG_G1333-DddtoxA-C

    Plasmid

    #171732

    Purpose

    ptpTALECD vector assembly, Entry clone 1 with attL2 and L3

    Depositor

    Shin-ichi Arimura

    Article

    Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

    Insert

    C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
    

    Use

    Multisite gateway entry vector

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. E1_pENTR_L1-L4_NI_G1333-DddtoxA-N

    Plasmid

    #171725

    Purpose

    ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

    Depositor

    Shin-ichi Arimura

    Article

    Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

    Insert

    C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
    

    Use

    Multisite gateway entry vector

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. E1_pENTR_L1-L4_NN_G1333-DddtoxA-N

    Plasmid

    #171729

    Purpose

    ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

    Depositor

    Shin-ichi Arimura

    Article

    Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

    Insert

    C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
    

    Use

    Multisite gateway entry vector

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. E3_pENTR_L3-L2_NG_G1333-DddtoxA-N

    Plasmid

    #171733

    Purpose

    ptpTALECD vector assembly, Entry clone 1 with attL2 and L3

    Depositor

    Shin-ichi Arimura

    Article

    Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

    Insert

    C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
    

    Use

    Multisite gateway entry vector

    Available Since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDONR221 CARD8 FIIND

    Plasmid

    #169963

    Purpose

    pDONR221 for Gateway shuttling. Contains the FIIND (L162M-P434, no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Gateway cloning vector

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Ad-HisFlag-USP19ER

    Plasmid

    #155244

    Purpose

    for generating adenoviruses expressing the ER isoform of rat USP19

    Depositor

    Simon Wing

    Article

    Wiles et al Mol Biol Cell. 2015 Mar 1;26(5):913-23.

    Insert

    USP19 (ER isoform) (Usp19 Rat)
    

    Use

    Adenoviral

    Tags

    6XHis-3XFlag

    Mutation

    H118R- please see dep. comments

    Promoter

    CMV

    Available Since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDGE473

    Plasmid

    #153264

    Purpose

    M1E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDGE500

    Plasmid

    #153273

    Purpose

    M6E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDGE502

    Plasmid

    #153275

    Purpose

    M8E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pOCC17

    Plasmid

    #118875

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal tagRFP

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C--NotI-ccdB-AscI-tagRFP-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease and tagRFP

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOCC16

    Plasmid

    #118870

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal EGFP

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C--NotI-ccdB-AscI-EGFP-stop-HindIII cassette
    

    Tags

    EGFP and HIS6, cleavable with 3C protease

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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