We narrowed to 8,865 results for: BLI;

1. TRIOBP-TwinStrepII

   Plasmid

   #83201

   Purpose

   Expression of tagged protein in mammalian cells

   Depositor

   Peter Barr-Gillespie

   Insert

   TRIOBP (Triobp Mouse)
   

   Use

   Tags

   Twin Strep Tag II

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLIX403-EBFP2-ccdB-G418

   Plasmid

   #158556

   Purpose

   Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

   Depositor

   Alejandro Chavez

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAc5.1B-EGFP-DmRRP40

   Plasmid

   #65797

   Purpose

   expresses EGFP-tagged RRP40 in Drosophila cells

   Depositor

   Elisa Izaurralde

   Insert

   RRP40 (Rrp40 Fly)
   

   Use

   Drosophila expression

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Actin 5C

   Available since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLenti-ePEG-LINE1-loxPsym

   Plasmid

   #216866

   Purpose

   Lentiviral guide RNA vector to insert the loxPsym sequence into LINE1 elements

   Depositor

   Leopold Parts

   Insert

   spacerLINE1_cr772scaffold_PBS-loxPsm-HA-tevopreQ
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. MIG-REST

   Plasmid

   #91898

   Purpose

   Expression of mouse REST

   Depositor

   Julien Sage

   Insert

   REST (Rest Mouse)
   

   Use

   Retroviral

   Tags

   ires-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV

   Available since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET MBP TEV expression vector with BrioBrick polypromoter restriction sites (14-Q)

   Plasmid

   #48311

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mouse ptc probe M2-3

   Plasmid

   #58701

   Purpose

   makes mouse ptc probe for in situ hybridization

   Depositor

   Matthew Scott

   Insert

   ptc (Ptch1 Mouse)
   

   Use

   In situ probe

   Tags

   Expression

   Bacterial

   Mutation

   only EcoRI fragment (5' end)

   Promoter

   T7

   Available since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pKSB- 3xP3-mTurqSV40 GGGG AAGA

   Plasmid

   #174533

   Purpose

   3xP3-mTurquoise fluorescence marker cassette for gene knock-in in mosquitoes

   Depositor

   Eric Marois

   Insert

   3xP3-mTurquoise BsaI cassette
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   3xP3

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Sperm whale Mb 64Y68F in pVP80K

   Plasmid

   #86956

   Purpose

   Increases expression of sperm whale myoglobin protein for making apomyoglobin as a reagent for measuring rates of hemin dissociation (as opposed to previous construct in pUC).

   Depositor

   John Olson

   Insert

   Mb 64Y68F
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   64Y68F

   Promoter

   T5

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pB-CAG-PE2-NatMX

    Plasmid

    #158567

    Purpose

    Piggybac vector that allows for the generation of PE2-expressing stable cell lines

    Depositor

    Alejandro Chavez

    Insert

    PE2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJM104: pTrc RFP on pCM66T

    Plasmid

    #74745

    Purpose

    pCM66T with promoter pTrc driving RFP expression (maxRFP = BBa_E1010)

    Depositor

    Mary Lidstrom

    Insert

    RFP (BioBrick part number BBa_E1010)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    pTrc

    Available since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKT-PDGFRa-IRES-Katushka

    Plasmid

    #133081

    Purpose

    Expresses PDGFRa receptor and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Insert

    PDGFRa (Pdgfra Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLIX403-EBFP2-ccdB-Blast

    Plasmid

    #158557

    Purpose

    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJK615

    Plasmid

    #71699

    Purpose

    Produces E. coli thioredoxin with a C-terminal His6 tag (EcTrxAH6)

    Depositor

    T. J. Kappock

    Insert

    thioredoxin
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLXIN2-RFP

    Plasmid

    #99205

    Purpose

    RFP expressing bicistronic retroviral vector

    Depositor

    Saroj Mathupala

    Insert

    Red fluorescent protein
    

    Use

    Retroviral; Bicistronic retroviral expression vec…

    Tags

    Expression

    Mammalian

    Mutation

    The dsRed cDNA was PCR amplified off AddGene plas…

    Promoter

    Available since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUA97

    Plasmid

    #69340

    Purpose

    It encodes a fluorescent protein that can be fused to a gene of interest using SEC CRISPR strategy

    Depositor

    Oliver Hobert

    Insert

    ceBFP SEC 3xFlag
    

    Use

    Tags

    3xFlag and ceBFP (mTagBFP)

    Expression

    Worm

    Mutation

    sqt-1(e1350)

    Promoter

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pExp-PpiB

    Plasmid

    #112572

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and PpiB (peptidyl-prolyl cis-trans isomerase B from E. coli) fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis-PpiB and Strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHH0103 NEDD4 WW domain #3

    Plasmid

    #104210

    Purpose

    Bacterial expression of WW domain #3 from NEDD4

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    NEDD4 WW domain #3 (NEDD4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHH0103 NEDD4L WW domain #2

    Plasmid

    #104205

    Purpose

    Bacterial expression of WW domain #2 from NEDD4L

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    NEDD4L WW domain #2 (NEDD4L Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGAL-flp(H305L)-NAT

    Plasmid

    #160380

    Purpose

    overexpression of Flp recombinase step-arrest mutant in yeast

    Depositor

    John McCusker

    Insert

    FLP1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    H305L

    Promoter

    GAL

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge