We narrowed to 4,183 results for: biorxiv

1. HA-MBP-TEVpCS-ADGRG4 CTFdeltaTA-FLAG

   Plasmid

   #217735

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRG4 (ADGRG4 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-2727

   Promoter

   Available since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. HA-MBP-TEVpCS-ADGRG3 CTFdeltaTA-FLAG

   Plasmid

   #217734

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRG3 (ADGRG3 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-255

   Promoter

   Available since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. HA-MBP-TEVpCS-ADGRG7 CTFdeltaTA-FLAG

   Plasmid

   #217738

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRG7 (ADGRG7 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-421

   Promoter

   Available since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-2xflox-BRX-eGFP-NLS

   Plasmid

   #217535

   Purpose

   AAV vector to drive the expression of eGFP in genetically defined cell populations under the control of the 4xBRE regulatory element of SMAD1 transcription factor and miniXon splicing casette in vivo

   Depositor

   Peter Scheiffele

   Article

   Okur et al bioRxiv 2023

   Insert

   4X BRE reporter and miniXon casette (Smad1 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   BMP response element (BRE)

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. 1114H

   Plasmid

   #200640

   Purpose

   An. gambiae transgenesis plasmid. Expresses gRNAs targeting B2-tubulin, ZPG, and DSXF. Crossing to Cas9 generates sterile males

   Depositor

   Omar Akbari

   Article

   Smidler et al bioRxiv [Preprint]. 2023 Jul 21:2023.07.

   Insert

   gRNA targeting DSXF, ZPG, and B2-tubulin. Actin5c-CFP and Vasa2-EYFP reporters.
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. piggybac-EF1-halo-Oct4

   Plasmid

   #197909

   Purpose

   single-particle tracking for OCT4

   Depositor

   James Zhe Liu

   Article

   Dong et al bioRxiv 2022.10.26.513917

   Insert

   pou5f1 (Pou5f1 Mouse)
   

   Use

   Tags

   Halotag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFPO-His-G-Ca-FLITS

   Plasmid

   #191455

   Purpose

   Bacterial isolation of genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

   Depositor

   Dorus Gadella

   Article

   van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

   Insert

   G-Ca-FLITS
   

   Use

   Tags

   His

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFR-mScarletI-antiFRET-P2A-GCaMP3

   Plasmid

   #191460

   Purpose

   Compare the intensity of the genetically encoded calcium sensor (GECI) GCaMP3 to mScarlet-I fluorescent protein

   Depositor

   Dorus Gadella

   Article

   van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

   Insert

   GCaMP3
   

   Use

   Tags

   His-mScarletI-antiFRETlinker-P2A

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFR-mTq2-antiFRET-P2A-K-GECO1

   Plasmid

   #191469

   Purpose

   Compare the intensity of the genetically encoded calcium sensor (GECI) K-GECO1 to mTurquoise2 fluorescent protein

   Depositor

   Dorus Gadella

   Article

   van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

   Insert

   K-GECO1
   

   Use

   Tags

   His-mTurquoise2-antiFRETlinker-P2A

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFR-mTq2-antiFRET-P2A-R-GECO1

    Plasmid

    #191471

    Purpose

    Compare the intensity of the genetically encoded calcium sensor (GECI) R-GECO1 to mTurquoise2 fluorescent protein

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    R-GECO1
    

    Use

    Tags

    His-mTurquoise2-antiFRETlinker-P2A

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Linked mRuby3 VLP

    Plasmid

    #182430

    Purpose

    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-GFP-mRuby3 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Inserts

    VP2C-GFP-mRuby3
    deltaVP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 and GAL10

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. Coexpressed mRuby3 VLP

    Plasmid

    #182431

    Purpose

    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-GFP (GAL10 promoter), and VP2C-mRuby3 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Inserts

    VP2C-GFP
    deltaVP1
    VP2C-mRuby3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1, GAL10, and GAL7

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Linked Free NES (M)

    Plasmid

    #182436

    Purpose

    Yeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Insert

    FPPS(M)-NES
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Coexpressed Free NES (M)

    Plasmid

    #182437

    Purpose

    Yeast integrative plasmid for expressing ERG20(F96W-N127W) (GAL10 promoter) and AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Inserts

    FPPS(M)
    NES
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10 and GAL7

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFR-mTq2-antiFRET-P2A-CH-GECO2.1

    Plasmid

    #191470

    Purpose

    Compare the intensity of the genetically encoded calcium sensor (GECI) CH-GECO2.1 to mTurquoise2 fluorescent protein

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    CH-GECO2.1
    

    Use

    Tags

    His-mTurquoise2-antiFRETlinker-P2A

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFR-mScarletI-antiFRET-P2A-jGCaMP7c

    Plasmid

    #191461

    Purpose

    Compare the intensity of the genetically encoded calcium sensor (GECI) jGCaMP7c to mScarlet-I fluorescent protein

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    jGCaMP7c
    

    Use

    Tags

    His-mScarletI-antiFRETlinker-P2A

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGlomyc-Ovol1

    Plasmid

    #172871

    Purpose

    Mammalian expression vector with myc-tagged mouse Ovol1 coding sequence

    Depositor

    Renee van Amerongen

    Article

    Mourao et al bioRxiv 2021.06.22.449461

    Insert

    Ovol1 (Ovol1 Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pETDuet1_6xHis-TEV-Lambda-Protein-Phosphatase

    Plasmid

    #223747

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    Lambda-PPase
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-hSyn1-FLEX-NEPLDCV-P2A-mRUBY3-WPRE

    Plasmid

    #207664

    Purpose

    Neuropeptide degradation

    Depositor

    Sung Han

    Article

    Kim et al bioRxiv 2023.01.19.524797

    Insert

    NEP (MME Human)
    

    Use

    AAV and Cre/Lox

    Tags

    mRUBY3

    Expression

    Mutation

    Addition of signaling peptide (POMC) on the N-ter…

    Promoter

    hSynapsin

    Available since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-hSyn-GRAB_g5-HT3.0

    Plasmid

    #208717

    Purpose

    Expresses the green 5-HT sensor GRAB_g5-HT3.0 in neurons

    Depositor

    Yulong Li

    Article

    Deng et al bioRxiv 2023.05.27.542566

    Insert

    Green fluorescent 5-HT sensor GRAB_g5-HT3.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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