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Showing: 2821 - 2840 of 16282 results

  1. pLVUT-spC3GFP

    Plasmid
    #225487
    Purpose
    Tet-on lentiviral vector for controlled expression of a secretable and permeable form of C. botulinum exoenzyme C3 transferase fused with EGFP
    Depositor
    Robert Gross
    Article
    Gutekunst et al Neuroscience. 2016 Dec 17;339:308-318. d
    Insert
    Secretable and permeable exoenzyme C3 transferase
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Amino acids 30-251
    Promoter
    Human ubiquitin C
    Available Since
    Sept. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    225487-plasmid-map-sequence-id-444518

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  4. pVioCD53

    Plasmid
    #191130
    Purpose
    Expression of vioC, truncated vioD and tdMCP form a J23105 constitutive promoter
    Depositor
    Ariel Lindner
    Article
    Guo et al Cell. 2022 Sep 29;185(20):3823-3837.e23.
    Inserts
    vioC (vioC Synthetic)
    vioD truncated
    dMCP off-frame
    Use
    Synthetic Biology
    Mutation
    C-terminal truncation from 264th amino acid
    Promoter
    J23105 constitutive promoter
    Available Since
    June 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    191130-plasmid-map-sequence-id-382518

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  5. NK85

    Plasmid
    #176611
    Purpose
    To minimize dimerization between FcRg transmembrane domains, as this cysteine forms a disulfide bridge between y chains. To manipulate ligand spacing.
    Depositor
    Meghan Morrissey
    Article
    Kern et al Elife. 2021 Jun 3;10. pii: 68311. doi: 1
    Insert
    FCERG
    Use
    Lentiviral
    Tags
    EGFP and HA, SNAP-tag
    Expression
    Mammalian
    Mutation
    C25A
    Available Since
    May 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    176611-plasmid-map-sequence-id-362592

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  7. pLB(N)CX AT-CH3-FLAG-HA WY

    Plasmid
    #133725
    Purpose
    Retroviral vector with CMV promoter driving mutant form of acetyltransferase and CH3 domains fragment of human p300 (EP300) (WY1466-1467AS in AT domain).
    Depositor
    James A. DeCaprio
    Article
    Borger et al J Virol. 2006 May . 80(9):4292-303.
    Insert
    EP300 (EP300 Human)
    Use
    Retroviral
    Tags
    Flag and HA
    Mutation
    partial (not complete) p300 fragment, AT and CH3 …
    Available Since
    March 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    133725-plasmid-map-sequence-id-276050

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  14. CROPseq-Guide-BSD

    Plasmid
    #216123
    Purpose
    Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the blasticidin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.
    Depositor
    Stefan Kubicek
    Article
    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    April 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216123-plasmid-map-sequence-id-428727

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  19. pMRX-IP-EGFP-PA-BAX(lyso, G108V)

    Plasmid
    #233345
    Purpose
    Stable expression of EGFP-LOV2(G528A, N538E)-BAX-TMEM106B in mammalian cells. The G108V mutation suppresses pore-forming activity of BAX.
    Depositor
    Noboru Mizushima
    Article
    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.
    Inserts
    LOV2 domain of phototropin1
    BAX (BAX Human)
    TMEM106B (TMEM106B Human)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    BAX(3-171), N-terminus and C-terminus are deleted…
    Available Since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233345-plasmid-map-sequence-id-469414

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Showing: 2821 - 2840 of 16282 results