We narrowed to 78,677 results for: TRI

1. L1_Cas9-Ck4

   Plasmid

   #136135

   Purpose

   L1 in position 4, for AtcoCas9 (Puchta) expression in L2 CRISPR vectors

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   p5-MpEF1a:Cas9_3tNos-35S
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. ABHD16A-mNG

   Plasmid

   #195157

   Purpose

   mammalian expression of ABHD16A tagged with mNeonGreen

   Depositor

   Gia Voeltz

   Article

   Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

   Insert

   ABHD16A (ABHD16A Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. L1_lacZgRNA-Ck2

   Plasmid

   #136137

   Purpose

   L1 in position 2, to clone gRNA target using BbsI, lacZ blue-white screening

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   p5-MpU6:lacZgRNA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pX330-UGCG-KO

   Plasmid

   #80010

   Purpose

   gRNA to knock out expression of UGCG gene. The product of this gene catalyzes the first step in synthesis of glycosphingolipids.

   Depositor

   Sriram Neelamegham

   Article

   Stolfa et al Sci Rep. 2016 Jul 26;6:30392. doi: 10.10

   Insert

   UGCG (UGCG Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLKO-shPKM2_4

   Plasmid

   #42516

   Depositor

   Dimitrios Anastasiou

   Article

   Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

   Insert

   shRNA-PKM2 (PKM Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMT-Hrp48-ADARcd-E488Q-V5

   Plasmid

   #139685

   Purpose

   Expresses the hyperactive form of the catalytic domain of the ADAR protein fused to HRP-48 for use in "TRIBE" assays

   Depositor

   Michael Rosbash

   Article

   Xu et al RNA. 2018 Feb;24(2):173-182. doi: 10.126

   Insert

   Hrp48, ADAR (Adar Fly)
   

   Use

   Tags

   V5

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Available since

   March 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTK88_GFP-Anillin

   Plasmid

   #46354

   Depositor

   Iain Cheeseman

   Article

   Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

   Insert

   Anillin (ANLN Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   a1854 is changed to c to disrupt XhoI site withou…

   Promoter

   pBABE

   Available since

   July 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCA24N-ligase-cTF

   Plasmid

   #87743

   Purpose

   IPTG-inducible expression of ligase-cTF fusion for protein purification

   Depositor

   Wayne Patrick

   Article

   Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

   Insert

   T4 DNA ligase (30 )
   

   Use

   Tags

   6xHis and cTF (chimeric transcription factor base…

   Expression

   Bacterial

   Mutation

   Promoter

   T5-lac

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCB168

   Plasmid

   #37389

   Depositor

   Iain Cheeseman

   Article

   Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

   Insert

   dynein cytoplasmic 1 intermediate chain 1 (DYNC1I1 Human)
   

   Use

   Retroviral

   Tags

   GFP, S-tag, and TEV

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAd-Track Flag-SIRT1

    Plasmid

    #8438

    Depositor

    Pere Puigserver

    Article

    Rodgers et al Nature 2005 Mar 3;434(7029):113-8.

    Insert

    SIRT1 (Sirt1 Mouse)
    

    Use

    Adenoviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAG_EGFP-RandE3

    Plasmid

    #79870

    Purpose

    Expresses under the CAG promoter RandE3, a synthetic E3 ligase with random residues in its binding domain; control construct for GFE3.

    Depositor

    Don Arnold

    Article

    Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

    Insert

    GFP-RandE3
    

    Use

    Synthetic Biology

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Beta-actin

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV_nLightG

    Plasmid

    #217656

    Purpose

    Expresses norepinephrine indicator nLightG under mammalian promoter

    Depositor

    Tommaso Patriarchi

    Article

    Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

    Insert

    nLightG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. hKv2.1-pEGFP-N1

    Plasmid

    #111532

    Purpose

    Expresses human KCNB1 in mammalian cells for imaging

    Depositor

    Federico Sesti

    Article

    Yu et al Cell Death Dis. 2017 Apr 6;8(4):e2737. d

    Insert

    human Kv2.1 (KCNB1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSB-CMV-MCS-puro PM-mKate2-P2A-GEM-LTB4mut

    Plasmid

    #202644

    Purpose

    Expresses the LTB4-insensitive control sensor GEM-LTB4mut along with the plasma membrane targeted mKate2 tag in mammalian cells

    Depositor

    Balázs Enyedi

    Article

    Tamas et al Nat Commun. 2023 Aug 1;14(1):4610. doi:

    Insert

    PM-mKate2-P2A-GEM-LTB4mut
    

    Use

    Tags

    PM-mKate2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Emi LC

    Plasmid

    #113666

    Purpose

    For transient expression of Emicizumab sequence (CAS# 1610943-06-0, KEGG# D10821), IgG4 bispecific antibody targeting FIX(a) & FX(a). Must be co-expressed with anti-FIX(a) and anti-FX(a) Emi HC's.

    Depositor

    Robert Peters

    Article

    Leksa et al J Thromb Haemost. 2019 Mar 18. doi: 10.1

    Insert

    Emicizumab light chain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti-CMV-GFP-2A-G(N2c)-WPRE

    Plasmid

    #194352

    Purpose

    Lentivirus expressing eGFP and Rabies Glycoprotein (G) from CVS-N2c strain

    Depositor

    Marco Tripodi

    Article

    Lee et al Nat Methods. 2023 Apr;20(4):580-589. doi

    Inserts

    Enhanced Green Fluorescent Protein (eGFP)
    Rabies CVS-N2c glycoprotein (G)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pQdC12a

    Plasmid

    #191356

    Purpose

    Clostridium CRISPRi plasmid with TetR-repressible dCas12a (LbCpf1) expression

    Depositor

    Andrew Tolonen

    Article

    Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

    Inserts

    PGusA2-TetO2/1-dCas12a (LbCpf1)
    miniPthl-tetR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PGus2A-TetO2/1 and miniPthl

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLL3.7_hsa-miR-34a

    Plasmid

    #25791

    Depositor

    Judy Lieberman

    Article

    Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

    Insert

    hsa-miR-34a (TP53 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 13, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCB163

    Plasmid

    #37388

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

    Insert

    Dynactin 2 (DCTN2 Human)
    

    Use

    Retroviral

    Tags

    GFP, S-tag, and TEV

    Expression

    Mammalian

    Mutation

    A7P

    Promoter

    Available since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL3-Promoter-4xBS_BHLHE40

    Plasmid

    #153064

    Purpose

    Four BHLHE40 consensus binding sites cloned upstream of luciferase reporter gene

    Depositor

    Silvia Monticelli

    Article

    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

    Insert

    4X consensus BHLHE40 binding sites
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pX-evoCas9

    Plasmid

    #107550

    Purpose

    Expresses the M495V/Y515N/K526E/R661Q (evoCas9) SpCas9 variant in mammalian cells

    Depositor

    Anna Cereseto

    Article

    Casini et al Nat Biotechnol. 2018 Jan 29. pii: nbt.40

    Insert

    Humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    M495V/Y515N/K526E/R661Q

    Promoter

    CBh

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGEX-6P-1-HsRCK_E

    Plasmid

    #146210

    Purpose

    Bacterial Expression of HsRck

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

    Insert

    HsRck (DDX6 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Twitch-1 pcDNA3

    Plasmid

    #49812

    Purpose

    Fluorescent reporter for calcium signaling

    Depositor

    Oliver Griesbeck

    Article

    Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

    Insert

    Twitch-1
    

    Use

    Tags

    ECFP and cpCit174

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

24. pMuLE_ENTR_CBF-GLuc_L3-L2

    Plasmid

    #113868

    Purpose

    Single pathway reporter; notch promoter-driven expression of secreted Gaussia luciferase; MultiSite Gateway entry vector.

    Depositor

    Manfred Ogris

    Article

    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

    Inserts

    CBF (notch dependent promoter)
    GLuc
    

    Use

    Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. gRNA-leu-HYB

    Plasmid

    #64332

    Purpose

    leu2 deletion gRNA cassette carried by pRS42H

    Depositor

    Yong-Su Jin

    Article

    Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

    Insert

    gBlock product of leu2 deletion gRNA cassette
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

26. pCold-TF-hPLEKHM1

    Plasmid

    #64146

    Purpose

    For protein expression in E.coli

    Depositor

    Tamotsu Yoshimori

    Article

    Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

    Insert

    PLEKHM1 (PLEKHM1 Human)
    

    Use

    Tags

    6xHis-tag and Trigger Factor (TF)

    Expression

    Bacterial

    Mutation

    Promoter

    cspA

    Available since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

27. pMuLE_ENTR_PTCH1-mTurquoise2_R4-R3

    Plasmid

    #113864

    Purpose

    Single pathway reporter; hedgehog promoter-driven expression of mTurquoise2; MultiSite Gateway entry vector.

    Depositor

    Manfred Ogris

    Article

    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

    Inserts

    PTCH1 (hedgehog dependent promoter)
    mTurquoise2
    

    Use

    Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. GST-Hsp90 C(626-732)

    Plasmid

    #22483

    Depositor

    William Sessa

    Article

    Fontana et al Circ Res. 2002 May 3. 90(8):866-73.

    Insert

    Hsp90alpha C domain (HSP90AA2P Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. gRNA-his-HYB

    Plasmid

    #64333

    Purpose

    his3 deletion gRNA cassette carried by pRS42H

    Depositor

    Yong-Su Jin

    Article

    Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

    Insert

    gBlock product of his3 deletion gRNA cassette
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

30. pEYFP-N1-eKR2

    Plasmid

    #115337

    Purpose

    Expresses enhanced sodium pump for light-driven extrusion of sodium; activation with green light; codon-optimized for human/mouse

    Depositor

    Peter Hegemann

    Article

    Grimm et al Sci Rep. 2018 Jun 18;8(1):9316. doi: 10.

    Insert

    eKR2
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge