We narrowed to 21,628 results for: ESC

1. pMT-HuC:Dendra2

   Plasmid

   #80904

   Purpose

   Injection into Zebrafish larvae together with Tol2-Transposase to achieve pan-neuronal expression of the photoconvertible fluorescent protein Dendra2

   Depositor

   Periklis Pantazis

   Article

   Mohr et al Nat Protoc. 2016 Dec;11(12):2419-2431. d

   Insert

   Dendra2 (elavl3 Synthetic)
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   HuC

   Available since

   Aug. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMRE-Tn7-156

   Plasmid

   #118570

   Purpose

   miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mCardinal
   

   Use

   Minitn7 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDuex2-iBB-GECO1

   Plasmid

   #194216

   Purpose

   Far-red fluorescent Ca2+ ion indicator for mammalian cell and bacterial expression.

   Depositor

   Robert Campbell

   Article

   Hashizume et al Protein Sci. 2022 Oct;31(10):e4440. doi:

   Insert

   iBB-GECO1
   

   Use

   Tags

   6xHis

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. CoxVIIIx2-OeNL(Ca2+)_18μ-pcDNA3

   Plasmid

   #111924

   Purpose

   Orange color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of OeNL(Ca2+)_18μ

   Depositor

   Takeharu Nagai

   Article

   Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

   Insert

   OeNL(Ca2+)_18μ
   

   Use

   Tags

   CoxVIIIx2

   Expression

   Mammalian

   Mutation

   E67D, E104D, D133E at CaM

   Promoter

   CMV

   Available since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-trGluc

   Plasmid

   #158959

   Purpose

   DNA donor plasmid containing repair template for Gaussia luciferase (Gluc) of bioluminescent repair reporter (BLRR).

   Depositor

   Charles P. Lai

   Article

   Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

   Insert

   trGluc
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Mito-mEosEM-E

   Plasmid

   #226335

   Purpose

   Photoswitchable fluorescent protein for correlative light-electron microscopy; mitochondria-targeted mEosEM-E for mammalian expression

   Depositor

   Pingyong Xu

   Article

   Peng et al Cells. 2022 Mar 23;11(7):1077. doi: 10.3

   Insert

   COX8A (COX8A Human)
   

   Use

   Tags

   mEosEM-E

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-hPOMC1-26-sbGLuc-P2A-dTomato

   Plasmid

   #176704

   Purpose

   neural activity dependent release of sbGLuc from presynaptic vesicles

   Depositor

   Ute Hochgeschwender

   Article

   Prakash et al Commun Biol. 2022 Jan 11;5(1):33. doi: 1

   Inserts

   Gaussia luciferase, slow burn
   dTomato
   

   Use

   AAV

   Tags

   P2A and hPOMC1-26

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR-CMV-TetO2_3C-Avi-His6

   Plasmid

   #113887

   Purpose

   Mammalian (inducible) protein expression, in vivo biotinylation

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Avi-His6

   Expression

   Mutation

   Promoter

   CMV-MIE-TetO2

   Available since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT2-7xTcf-NLS-CNL

   Plasmid

   #65712

   Purpose

   Tol2-based Wnt signal reporter plasmid (expresses NLS-CNL)

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   7xTcf, minCMV, 3xNLS, CNL
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMRE-Tn7-130

    Plasmid

    #118548

    Purpose

    miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    TagBFP2
    

    Use

    Minitn7 delivery vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAD-KPS12-Pman

    Plasmid

    #133832

    Purpose

    Fluorescent protein mKate(KPS12) variant for optimized expression in Bacillus mycoides; under control of mannose inducible promoter.

    Depositor

    Oscar Kuipers

    Article

    Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

    Insert

    mKate(KPS12)
    

    Use

    E.coli/bacillus spp. shuttle vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pman

    Available since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR-CMV-TetO2_3C-mVenus-Twin-Strep

    Plasmid

    #113891

    Purpose

    Mammalian (inducible) protein expression, C-terminal 3C-mVenus-Twin-Strep fusion

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-mVenus-Twin-Strep

    Expression

    Mutation

    Promoter

    CMV-MIE-TetO2

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-20b-M-Ntx-S1

    Plasmid

    #58716

    Purpose

    Expresses in Escherichia coli Notexin with a N-terminal extra methionine and with the amino acid substitution N1S

    Depositor

    Fiorella Tonello

    Article

    Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14

    Insert

    Notexin
    

    Use

    Tags

    Methionine

    Expression

    Bacterial

    Mutation

    Changed Asparagine 1 to Serine

    Promoter

    T7

    Available since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMRE-Tn7-167

    Plasmid

    #118579

    Purpose

    miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mClover3
    

    Use

    Minitn7 delivery vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMRE-Tn5-152

    Plasmid

    #118534

    Purpose

    miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    sGFP2
    

    Use

    Minitn5 delivery vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMRE-Tn7-147

    Plasmid

    #118563

    Purpose

    miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mClover3
    

    Use

    Minitn7 delivery vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMRE-Tn5-157

    Plasmid

    #118539

    Purpose

    miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mClover3
    

    Use

    Minitn5 delivery vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-CMV-TetO2_HA-BirA

    Plasmid

    #113896

    Purpose

    Mammalian (inducible) expression of HA-tagged cytosolic E. coli biotin ligase

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Insert

    E. coli biotin ligase
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    CMV-MIE-TetO2

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-FlaviA-GFP-puro

    Plasmid

    #140088

    Purpose

    Fluorescence activatable reporter of flavivirus NS2B-NS3 protease activity

    Depositor

    Jorge Luis Arias-Arias

    Article

    Arias-Arias et al J Biol Chem. 2020 Jan 9. pii: RA119.0113

    Insert

    FlaviA-GFP
    

    Use

    Lentiviral; Low expression

    Tags

    6XHis tag and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. Carl-CeNL(Ca2+)_110μ-KDEL-pcDNA3

    Plasmid

    #111925

    Purpose

    Cyan color luminescent Ca2+ indicator targeted to endoplasmic reticulum. Calreticulin and a KDEL signal for ER retention located at the N-terminus and C-terminus of CeNL(Ca2+)_110μ

    Depositor

    Takeharu Nagai

    Article

    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

    Insert

    CeNL(Ca2+)_110μ
    

    Use

    Tags

    Calreticulin and KDEL

    Expression

    Mammalian

    Mutation

    E31D, F92W, E104D. D133E at CaM

    Promoter

    CMV

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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