We narrowed to 10,981 results for: Vars;

1. PKAcs-GGSD5X-GFP

   Plasmid

   #108584

   Purpose

   Expresses PKAcs-GFP fusion in mammalian cells with flexible linker

   Depositor

   Robert Hughes

   Article

   Norris et al FEBS Open Bio. 2018 Apr 25;8(6):1029-104

   Insert

   protein kinase A, catalytic subunit
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   H87Q and W196R

   Promoter

   Available since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUC18/promEco/Lysostaphin

   Plasmid

   #125765

   Purpose

   Production of staphylolytic protein lysostaphin from a constitutive promoter

   Depositor

   Benedykt Wladyka

   Article

   Madry et al Mol Biotechnol. 2019 Oct;61(10):774-782.

   Insert

   lysostaphin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   sequence corresponding to the mature form protein

   Promoter

   pemIK-Sa1 promoter

   Available since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. HA GSK3 beta wt pcDNA3

   Plasmid

   #14753

   Purpose

   Mammalian expression of human GSK3 beta with HA tag

   Depositor

   Jim Woodgett

   Article

   He et al Nature. 1995 Apr 13. 374(6523):617-22.

   Insert

   GSK3 beta (GSK3B Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 12, 2007

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pCas9

   Plasmid

   #167547

   Purpose

   Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.

   Depositor

   Howard Hang

   Article

   Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00

   Inserts

   chloramphenicol acetyl transferase
   pUC19 origin of replication
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAB061i

   Plasmid

   #79226

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   Ppro1 rpoZ-HA4; PlacIq lacI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ppro1; PlacIq

   Available since

   Oct. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAB061i3

   Plasmid

   #79230

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   Ppro1 rpoZ; PlacIq lacI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ppro1; PlacIq

   Available since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Lenti-PEAR-mCherry

   Plasmid

   #228892

   Purpose

   All-in-one lentiviral version of the PEAR reporter. Constitutive RFP expression with GFP turned on in event of prime editing.

   Depositor

   Francisco J Sánchez-Rivera

   Article

   Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

   Insert

   EGFP-IRES-mScarlet
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAB078d5

   Plasmid

   #79204

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PlacZ-opt (OR1) luxAB; Ppro1 434cI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PlacZ-opt (OR1); Ppro1

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJC175e

   Plasmid

   #79219

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PPSP gIII, luxAB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PPSP

   Available since

   April 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AAV-ReaChR-citrine

    Plasmid

    #50954

    Purpose

    ReaChR-citrine in AAV2 vector under human synapsin promoter

    Depositor

    Roger Tsien

    Article

    Lin et al Nat Neurosci. 2013 Oct;16(10):1499-508.

    Insert

    ReaChR-citrine
    

    Use

    AAV

    Tags

    citrine

    Expression

    Mutation

    Promoter

    truncated human Synapsin promoter

    Available since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LT3GEPIR

    Plasmid

    #111177

    Purpose

    Tet-ON miR-E (miR-30 variant)-based RNAi

    Depositor

    Johannes Zuber

    Article

    Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

    Insert

    miR-E (miR-30 variant)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. piggyBAC-H2B mCherry-XL (R125K)

    Plasmid

    #189926

    Purpose

    Expresses nuclear-localized mCherry-XL (R125K) in mammalian cells

    Depositor

    Ralph Jimenez

    Article

    Mukherjee et al J Phys Chem B. 2022 Jun 16. doi: 10.1021

    Insert

    mCherry-XL (R125K)
    

    Use

    Tags

    H2B

    Expression

    Mammalian

    Mutation

    differs from mCherry by R125K, W143S, I161V, Q163…

    Promoter

    CMV7

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pmCherry-SopF

    Plasmid

    #135174

    Purpose

    Expresses an mCherry-SopF fusion in mammalian cells

    Depositor

    Leigh Knodler

    Article

    Lau et al PLoS Pathog. 2019 Jul 24;15(7):e1007959.

    Insert

    SopF (STM1239 Salmonella enterica serovar Typhimurium)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. eSrtA(2A-9) pET29b

    Plasmid

    #75145

    Purpose

    reprogrammed sortase A variant that recognizes alternative substrate LAXTG with high activity and specificity

    Depositor

    David Liu

    Article

    Dorr et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

    Insert

    reprogrammed sortase A variant
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    S102C A104H E105D K138P K152I N160K K162H T164N K…

    Promoter

    T7

    Available since

    July 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. EGFP-Tubulin-6

    Plasmid

    #56450

    Purpose

    Localization: Microtubules, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Tubulin (TUBA1B Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. eSrtA(4S-9) pET29b

    Plasmid

    #75146

    Purpose

    reprogrammed sortase A variant that recognizes alternative substrate LPXSG with high activity and specificity

    Depositor

    David Liu

    Article

    Dorr et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

    Insert

    reprogrammed sortase A variant
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    N98D S102C A104V A118T F122A K134R F144L I182V E1…

    Promoter

    T7

    Available since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAGGS_VP35_MARV

    Plasmid

    #135471

    Purpose

    Encodes Marburg virus VP35 gene under control of the AG promoter

    Depositor

    Elke Mühlberger

    Article

    Schmidt et al J Infect Dis. 2011 Nov;204 Suppl 3:S861-

    Insert

    VP35
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    AG

    Available since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAGGS_NP_MARV

    Plasmid

    #135470

    Purpose

    Encoding Marburg virus NP gene under control of the AG promoter

    Depositor

    Elke Mühlberger

    Article

    Schmidt et al J Infect Dis. 2011 Nov;204 Suppl 3:S861-

    Insert

    nucleoprotein (NP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    AG

    Available since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p2T-CMV-evoCjCas9-PEmax_dRnH-BlastR

    Plasmid

    #194063

    Purpose

    Mammalian expression of evoCjCas9 PEmax_deltaRnH

    Depositor

    Gerald Schwank

    Article

    Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

    Insert

    evoCjCas9-PEmax_dRnH
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pArk313

    Plasmid

    #200257

    Purpose

    Ark313 rep/cap

    Depositor

    Justin Eyquem

    Article

    Nyberg et al Cell. 2023 Jan 19;186(2):446-460.e19. do

    Insert

    Ark313 Rep/Cap
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge