We narrowed to 42,665 results for: cha

1. pCfB8792

   Plasmid

   #126910

   Purpose

   Template for PCR amplification of ADE2 gRNA biobrick for targeting Saccharomyces cerevisiae ADE2 gene

   Depositor

   Irina Borodina

   Article

   Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

   Insert

   ADE2 gRNA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pES.HSV2.3a

   Plasmid

   #69593

   Purpose

   TCR a chain construct for generation of gBT-I mice

   Depositor

   Francis R. Carbone

   Article

   Mueller et al Immunol Cell Biol. 2002 Apr;80(2):156-63

   Insert

   TCR alpha chain cDNA from clone HSV2.3 (Tcra Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H-2Kb

   Available since

   Oct. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pCfB5191

   Plasmid

   #126911

   Purpose

   Plasmid containing gRNA expression cassettes targeting the X-4 and XII-5 integration sites in Saccharomyces cerevisiae

   Depositor

   Irina Borodina

   Article

   Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

   Insert

   X-4 and XII-5 gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET/LEGH

   Plasmid

   #108226

   Purpose

   Bacterial expression of EGFP with N-terminal lanthanide binding tag and C-terminal His6 tag

   Depositor

   Didier Picard

   Article

   Bhattacharya et al Sci Rep. 2018 Feb 12;8(1):2801. doi: 10.

   Insert

   LBT-EGFP-His6
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7, lacO

   Available since

   April 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNM3749

   Plasmid

   #154108

   Purpose

   loxP FRT FRT3 RMCE landing site in a Golden Gate SapI GCG ACG assembly vector

   Depositor

   Michael Nonet

   Article

   Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

   Inserts

   LoxP-SEC-LoxP
   FRT::rpl-28p::GFP::his-58::FRT3
   

   Use

   Cre/Lox; Rmce

   Tags

   Expression

   Worm

   Mutation

   Promoter

   not applicable

   Available since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKH-MoHyPer

   Plasmid

   #78267

   Purpose

   Express HyPer-AS sensor that change excitation wavelength with the presence of hydrogen peroxide.

   Depositor

   Nicole Donofrio, Jeffrey Caplan

   Article

   Huang et al Mol Plant Pathol. 2016 Mar 7. doi: 10.11

   Insert

   MoHyper
   

   Use

   Express in magnaporthe

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Rp27

   Available since

   Aug. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pCASP-HilA empty vector

   Plasmid

   #153333

   Purpose

   Triggers HilA overexpression from Salmonella upon induction with arabinose without any synthetic protein expressed

   Depositor

   Josef Penninger

   Article

   Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

   Type

   Empty backbone

   Use

   Salmonella expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. bactin:GFP-DCX

   Plasmid

   #105961

   Purpose

   transgenesis, ubiquitous expression

   Depositor

   Caren Norden

   Article

   Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

   Insert

   DCX (DCX Human)
   

   Use

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pASH3 [pmyo-3::BeCNG1-YFP]

   Plasmid

   #168167

   Purpose

   Expression of BeCNG1-YFP in BWMs of C. elegans

   Depositor

   Alexander Gottschalk

   Article

   Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

   Insert

   BeCNG1-YFP
   

   Use

   Tags

   YFP

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAct:dCas9-scFv

    Plasmid

    #78900

    Purpose

    Expresses scFv-VP64 under actin promoter for SunTag CRISPRa in Drosophila cells

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    scFV-VP64
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Insect

    Mutation

    Promoter

    pActin (Drosophila)

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB_TRE_sg83-PDGFR_EF1a_iRFP670

    Plasmid

    #186311

    Purpose

    Plasmid for inducible mammalian cell expression of the sg83 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line construction

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    sg83-PDGFR
    iRFP670
    

    Use

    Tags

    His, myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PB_TRE_sg88-PDGFR_EF1a_eBFP2

    Plasmid

    #186312

    Purpose

    Plasmid for inducible mammalian cell expression of the sg88 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    sg88-PDGFR
    eBFP2
    

    Use

    Tags

    His, myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PB_TRE_P9-PDGFR_EF1a_mPlum

    Plasmid

    #186307

    Purpose

    Plasmid for inducible mammalian cell expression of the P9 helixCAM and constitutive expression of an identifying fluorescent protein (mPlum). Flanked with PiggyBac sites for easy line construction.

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    P9-PDGFR
    mPlum
    

    Use

    Tags

    HA, myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PB_TRE_AP10-PDGFR_EF1a_mOrange

    Plasmid

    #186308

    Purpose

    Plasmid for inducible mammalian cell expression of the AP10 helixCAM and constitutive expression of an identifying fluorescent protein (mOrange). Flanked with PiggyBac sites for easy line construction

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    AP10-PDGFR
    mOrange
    

    Use

    Tags

    HA, myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PB_TRE_P3-PDGFR_EF1a_iRFP670

    Plasmid

    #186305

    Purpose

    Plasmid for inducible mammalian cell expression of the P3 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line construction.

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    P3-PDGFR
    iRFP670
    

    Use

    Tags

    HA, myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEM-rat FceRI beta cDNA

    Plasmid

    #8374

    Depositor

    Jean Kinet

    Article

    Kinet et al Proc Natl Acad Sci U S A 1988 Sep;85(17)

    Insert

    rat Fc epsilon RI beta chain (Ms4a2 Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 7, 2005

    Availability

    Academic Institutions and Nonprofits only

17. VV221: sdChR(C138S)-TS-eGFP-ER in fck

    Plasmid

    #58513

    Purpose

    Expresses sdChR with the C138S (step function opsin) mutation fused to eGFP under the a-CamKII promoter

    Depositor

    Adam Cohen

    Article

    Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

    Insert

    sdChR(C138S)-TS-eGFP-ER
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    changed Cysteine 138 to Serine

    Promoter

    a-CamKII

    Available since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAct:MS2-p65-HSF

    Plasmid

    #78902

    Purpose

    Expresses MS2-p65-HSF under actin promoter for SAM CRISPRa in Drosophila cells

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    MS2-p65-HSF
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    pActin (Drosophila)

    Available since

    Sept. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCFD3-MS2tail-SAM

    Plasmid

    #78905

    Purpose

    Vector for expressing a single sgRNA with SAM MS2 tail, under U6:3 promoter

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCVL SFFV Sbf-Sal linker

    Plasmid

    #50434

    Purpose

    Cloning vector for coexpression of gene cloned in between Sbf and Sal sites with BFP in mammalian cells. Made for cloning of meganuclease for direct comparison with megaTALs (pCVL TAL Xba-Sal).

    Depositor

    Andrew Scharenberg

    Article

    Boissel et al Nucleic Acids Res. 2013 Nov 26.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    May 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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