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We narrowed to 7,295 results for: alp

Showing: 7151 - 7200 of 7295 results

  4. Flag_MmPERK_S503_N1114_pBabePuro

    Plasmid
    #58423
    Purpose
    retroviral pBabe puro vector encoding N-terminally FLAG tagged mutant mouse PERK deleted from amino acids M1 to Y502. The remaining amino acids are S503 to N1114.
    Depositor
    David Ron
    Article
    Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11
    Insert
    PERK (Eif2ak3 Mouse)
    Use
    Retroviral
    Tags
    FLAG and preprotrypsin signal sequence
    Expression
    Mammalian
    Mutation
    Deleted amino acids M1 to Y502
    Promoter
    Available since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    58423-plasmid-map-sequence-id-123700

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  5. pLL3.7-EF-EYFP-YAP1_delta5C-PolyA

    Plasmid
    #112289
    Purpose
    Fluorescently labels human YAP1-2gamma isoform (504 aa) delta5C mutant lacking the last five residues (FLTWL) in the PDZ binding domain at the N-terminus of the protein
    Depositor
    Erik Sahai
    Article
    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.
    Insert
    EYFP-YAP1 (YAP1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Human YAP1-2gamma lacking the last 5 Amino acids …
    Promoter
    EF-1 alpha
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    112289-plasmid-map-sequence-id-213521

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  6. pMIG-AVITEV- hNFATc1/aA

    Plasmid
    #74057
    Purpose
    retroviral expression plasmid for human NFATc1/aA with N-terminal BirA-biotinylation signal and TEV celavage site
    Depositor
    Ria Baumgrass
    Article
    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.
    Insert
    human NFATc1, isoform alpha-A (NFATC1 Human)
    Use
    Retroviral
    Tags
    AVI-TEV
    Expression
    Mammalian
    Mutation
    mutation G1157A [R235Q] and the silent mutation C…
    Promoter
    pMSCV-LTRs
    Available since
    June 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    74057-plasmid-map-sequence-id-142263

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  7. pLL3.7-EF-EYFP-YAP1_Y3F-PolyA

    Plasmid
    #112288
    Purpose
    Fluorescently labels human YAP1-2gamma isoform (504 aa) Y3F mutant _ tyrosines 341, 357 and 394 corresponding to tyrosines 391, 407 and 444 in this isoform
    Depositor
    Erik Sahai
    Article
    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.
    Insert
    EYFP-YAP1 (YAP1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    3 Tyrosines (341, 357 and 394 corresponding to ty…
    Promoter
    EF-1 alpha
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    112288-plasmid-map-sequence-id-213531

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  11. pUCIDT-attL1-Worm ABeta-attR5

    Plasmid
    #160437
    Purpose
    Entry vector for cloning nematode-codon-optimized Amyloid Beta(1-42) using 2-fragment gateway recombination
    Depositor
    Nicholas Tolwinski
    Article
    Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi
    Insert
    Homo sapiens amyloid beta precursor protein (APP Human, Nematode)
    Use
    Tags
    Cleavage signal
    Expression
    Bacterial
    Mutation
    Codon-optimized for expression in C. elegans
    Promoter
    Available since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    160437-plasmid-map-sequence-id-314980

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  13. pTM-RBDv2

    Plasmid
    #162785
    Purpose
    SARS-CoV-2 S protein receptor binding domain (RBD) expression in mammalian cells
    Depositor
    Ivan Vorobiev
    Article
    Sinegubova et al PLoS One. 2021 Feb 2;16(2):e0242890. doi
    Insert
    SARS-CoV-2 S protein receptor binding domain (RBD) (S Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2))
    Use
    Tags
    10xHis, c-myc, and hTPA leader
    Expression
    Mammalian
    Mutation
    Promoter
    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…
    Available since
    July 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162785-plasmid-map-sequence-id-333799

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  17. pT/UBC-mVenus-P2A-NRASG12V

    Plasmid
    #236074
    Purpose
    Sleeping Beauty transposon plasmid for hydrodynamic tail vein injection (HDTVi) into mice. Ubc promotor driven expression of mVenus and mutant NRAS (NRASG12V).
    Depositor
    Masashi Narita
    Article
    Chan et al Nature. 2024 Sep;633(8030):678-685. doi:
    Inserts
    mVenus
    NRAS G12V (NRAS Human)
    Use
    Sleeping beauty transposon plasmid
    Tags
    Expression
    Mutation
    G12V
    Promoter
    Ubc
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    236074-plasmid-map-sequence-id-472894

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  18. pT/CAGGS-NRASG12V/D38A-IRES-mVenus

    Plasmid
    #236077
    Purpose
    Sleeping Beauty transposon plasmid for hydrodynamic tail vein injection (HDTVi) into mice. Caggs promotor driven expression of mVenus and a double mutant NRAS (NRASG12VD38A).
    Depositor
    Masashi Narita
    Article
    Chan et al Nature. 2024 Sep;633(8030):678-685. doi:
    Inserts
    mVenus
    NRAS G12V D38A (NRAS Human)
    Use
    Sleeping beauty transposon plasmid
    Tags
    Expression
    Mutation
    G12V D38A
    Promoter
    CAGGS
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    236077-plasmid-map-sequence-id-472893

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  19. pT/CAGGS-NRASG12V-IRES-mVenus

    Plasmid
    #236076
    Purpose
    Sleeping Beauty transposon plasmid for hydrodynamic tail vein injection (HDTVi) into mice. Caggs promotor driven expression of mVenus and mutant NRAS (NRASG12V).
    Depositor
    Masashi Narita
    Article
    Chan et al Nature. 2024 Sep;633(8030):678-685. doi:
    Inserts
    mVenus
    NRAS G12V (NRAS Human)
    Use
    Sleeping beauty transposon plasmid
    Tags
    Expression
    Mutation
    G12V
    Promoter
    CAGGS
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    236076-plasmid-map-sequence-id-472892

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  20. pT/PGK-mVenus-P2A-NRASG12V

    Plasmid
    #236073
    Purpose
    Sleeping Beauty transposon plasmid for hydrodynamic tail vein injection (HDTVi) into mice. Pgk promotor driven expression of mVenus and mutant NRAS (NRASG12V).
    Depositor
    Masashi Narita
    Article
    Chan et al Nature. 2024 Sep;633(8030):678-685. doi:
    Inserts
    mVenus
    NRAS G12V (NRAS Human)
    Use
    Sleeping beauty transposon plasmid
    Tags
    Expression
    Mutation
    G12V
    Promoter
    PGK
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    236073-plasmid-map-sequence-id-472890

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  21. GFP Cav2.2 human HAgHA-plus exon 18a - Short-Cterm - Arginine -in pcDNA3

    Plasmid
    #198915
    Purpose
    N-terminal GFP tagged, exofacial HAgHA tag in Domain II, contains exon 18a, Short form of alternatively spliced C-teminus, SNP rs2278973 variant Arginine, in pcDNA3 vector
    Depositor
    Annette Dolphin
    Article
    Dahimene et al Function (Oxf). 2023 Oct 19;5(1):zqad060
    Insert
    Cav2.2 (CACNA1B Human)
    Use
    Tags
    N-terminal GFP, internal double HA
    Expression
    Mammalian
    Mutation
    snp rs2278973 Arginine
    Promoter
    CMV
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    198915-plasmid-map-sequence-id-392113

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  27. AAV-hSyn-scFVDnmt3AL_bGHpA

    Plasmid
    #177348
    Purpose
    AAV expression of a fusion protein with scFV, catalytic domain of Dnmt3a and C terminal domain of Dnmt3l from Synapisin promoter for targeted DNA methylation editing
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Insert
    catalytic domain of DNA Methyltransferase 3 Alpha (Dnmt3a Mouse)
    Use
    AAV
    Tags
    C-terminal domain of mouse Dnmt3l (194–415 aa), P…
    Expression
    Mammalian
    Mutation
    598–908 aa of mouse Dnmt3a
    Promoter
    human Synapsine
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177348-plasmid-map-sequence-id-353661

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  28. AAV-TRE-scFVDnmt3AL_bGHpA

    Plasmid
    #177354
    Purpose
    AAV expression of a fusion protein with scFV, catalytic domain of Dnmt3a and C terminal domain of Dnmt3l from doxycycine-inducible promoter for targeted DNA methylation editing
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Insert
    catalytic domain of DNA Methyltransferase 3 Alpha (Dnmt3a Mouse)
    Use
    AAV
    Tags
    C-terminal domain of mouse Dnmt3l (194–415 aa), P…
    Expression
    Mammalian
    Mutation
    598–908 aa of mouse Dnmt3a
    Promoter
    Tetracycline-dependent promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177354-plasmid-map-sequence-id-353614

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  29. AAV-TRE-scFVDnmt3A_bGHpA

    Plasmid
    #177355
    Purpose
    AAV expression of scFV-fused catalytic domain of Dnmt3a from doxycycine-inducible promoter for targeted DNA methylation editing
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Insert
    catalytic domain of DNA Methyltransferase 3 Alpha (Dnmt3a Mouse)
    Use
    AAV
    Tags
    myc and scFV
    Expression
    Mammalian
    Mutation
    598–908 aa of mouse Dnmt3a
    Promoter
    Tetracycline-dependent promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177355-plasmid-map-sequence-id-353667

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  31. SHIP2-C1

    Plasmid
    #214905
    Purpose
    expression of the truncated variants of the SHIP2 that retains second proline-rich domain and C-terminal sterile alpha motif
    Depositor
    Pavel Krejčí
    Article
    Fafilek et al Sci Signal. 2018 Sep 18;11(548):eaap8608
    Insert
    human SHIP2 aa 740-1258 (INPPL1 Human)
    Use
    Tags
    V5/His
    Expression
    Mammalian
    Mutation
    delta 1-739aa
    Promoter
    CMV
    Available since
    March 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214905-plasmid-map-sequence-id-426097

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  33. GFP Cav2.2 HA EI,II,III,IVA (E314A, E664A, E1370A, E1658A) pcDNA3

    Plasmid
    #206093
    Purpose
    Cav2.2 calcium channel with a N-terminal GFP tag, exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surface
    Depositor
    Annette Dolphin
    Article
    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi
    Insert
    cacna1b (CACNA1B Rabbit)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    E314A, E664A, E1370A, E1658A
    Promoter
    CMV
    Available since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    206093-plasmid-map-sequence-id-415677

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  34. Cav2.2 HA EI,II,III,IVA (E314A, E664A, E1370A, E1658A) pCAGGS

    Plasmid
    #206098
    Purpose
    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surface
    Depositor
    Annette Dolphin
    Article
    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi
    Insert
    cacna1b (CACNA1B Rabbit)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E314A, E664A, E1370A, E1658A
    Promoter
    CMV/B-actin
    Available since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    206098-plasmid-map-sequence-id-415670

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  42. pD649-HAsp-FCAR-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156619
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    FCAR (FCAR Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156619-plasmid-map-sequence-id-302347

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  43. pD649-HAsp-ULBP2-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156596
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    ULBP2 (ULBP2 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    156596-plasmid-map-sequence-id-383688

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  45. pD649-HAsp-CD8A-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156553
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    CD8A (CD8A Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    Aug. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    156553-plasmid-map-sequence-id-368527

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  49. pLEX307_CR-PDHA2S291D

    Plasmid
    #115200
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291D into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')
    Depositor
    Mohan Babu
    Article
    Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318
    Insert
    CR (CRISPR/Cas9-resistant)-PDHA2S291D (PDHA2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    c.321C>T (silent when translated to protein, c…
    Promoter
    EF1alpha
    Available since
    July 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    115200-plasmid-map-sequence-id-232426

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  50. pLEX307_CR-PDHA2S293D

    Plasmid
    #115201
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S293D into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')
    Depositor
    Mohan Babu
    Article
    Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318
    Insert
    CR (CRISPR/Cas9-resistant)-PDHA2S293D (PDHA2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    c.321C>T (silent when translated to protein, c…
    Promoter
    EF1alpha
    Available since
    July 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    115201-plasmid-map-sequence-id-231792

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Showing: 7151 - 7200 of 7295 results