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TRCN0000279887

Plasmid

#78153

Purpose

shCDK4 targeting CDS

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

CDK4 (CDK4 Human)

Use

Lentiviral and RNAi

Promoter

hU6 and hU6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
TRCN0000279952

Plasmid

#78155

Purpose

shCDK4 targeting 3UTR

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

CDK4 (CDK4 Human)

Use

Lentiviral and RNAi

Promoter

hU6 and hU6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
TRCN0000279953

Plasmid

#78154

Purpose

shCDK4 targeting CDS

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

CDK4 (CDK4 Human)

Use

Lentiviral and RNAi

Promoter

hU6 and hU6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
MAPK15 gRNA (BRDN0001145787)

Plasmid

#75618

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK15

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK15 (MAPK15 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAPK15 gRNA (BRDN0001148491)

Plasmid

#75619

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK15

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK15 (MAPK15 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAPK15 gRNA (BRDN0001162376)

Plasmid

#75620

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK15

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK15 (MAPK15 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAPK15 gRNA (BRDN0001162374)

Plasmid

#75621

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK15

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK15 (MAPK15 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMIR-Report-RASA1-3'UTR

Plasmid

#62575

Purpose

Translational Luciferase Reporter containing a 926 bp fragment of the RASA1 3'UTR.

Depositor

Michael Ruppert

Article

Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

Insert

RASA1 (RASA1 Human)

Use

Luciferase

Tags

None

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
CDK5R1 gRNA (BRDN0001147589)

Plasmid

#77852

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK5R1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK5R1 (CDK5R1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pGIPZ EF1a myc tagged mbIL15 IRES miRFP720-P2A-Blast

Plasmid

#248032

Purpose

Constitutively expresses myc tagged membrane bound IL15, miRFP720 and a blasticidin resistance gene in human cells

Depositor

Joshua Leonard

Article

Deol et al GEN Biotechnol. 2023 Jun 1;2(3):228-246.

Insert

myc-tagged membrane bound IL15 (IL15 Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Promoter

human EF1a

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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mCerulean-DNAJC5

Plasmid

#246730

Purpose

Mammalian expression of human DNAJC5 wildtype with a mCerulean tag on its N-terminus

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2025 Aug 25. doi: 10.1038

Insert

DNAJC5 (DnaJ heat shock protein family) (DNAJC5 Human)

Tags

mCerulean

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 23, 2025

Availability

Academic Institutions and Nonprofits only

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mCerulean-DNAJC5 L115R

Plasmid

#246732

Purpose

Mammalian expression of human DNAJC5 L115R with a mCerulean tag on its N-terminus

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2025 Aug 25. doi: 10.1038

Insert

DNAJC5 (DnaJ heat shock protein family) (DNAJC5 Human)

Tags

mCerulean

Expression

Mammalian

Mutation

L115R

Promoter

CMV

Available Since

Dec. 23, 2025

Availability

Academic Institutions and Nonprofits only

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mCerulean-DNAJC5 delL116

Plasmid

#246731

Purpose

Mammalian expression of human DNAJC5 L116 deleted with a mCerulean tag on its N-terminus

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2025 Aug 25. doi: 10.1038

Insert

DNAJC5 (DnaJ heat shock protein family) (DNAJC5 Human)

Tags

mCerulean

Expression

Mammalian

Mutation

delL116

Promoter

CMV

Available Since

Dec. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV6 FLAG-DNAJC5

Plasmid

#246740

Purpose

Mammalian expression of human DNAJC5 with L116 deleted, and a FLAG tag on its N-terminus

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2025 Aug 25. doi: 10.1038

Insert

DNAJC5 (DnaJ heat shock protein family) (DNAJC5 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 GST-SHP2 H116A E252A

Plasmid

#244493

Purpose

Bacterial Expression of pH insensitive SHP2 mutant

Depositor

Katharine White

Article

Van Dyck et al Sci Signal. 2025 Nov 11;18(912):eadt3018

Insert

PTPN11 (PTPN11 Human)

Tags

GST

Expression

Bacterial

Mutation

changed Histidine 116 to Alanine and Glutamate 25…

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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PAX3::FOXO1-2A-sfGFP

Plasmid

#240098

Purpose

human PAX3::FOXO1 in -2A-sfGFP backbone (Plasmid# 74668)

Depositor

Genevieve Kendall

Article

Kucinski et al Cell Rep. 2025 Jul 22;44(7):115923. doi:

Insert

PAX3::FOXO1 (FOXO1, PAX3 Human)

Expression

Mammalian

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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PB_rtTA_BsmB-HNRNPK_sgRNA_4

Plasmid

#242899

Purpose

PiggyBac cargo vector with HNRNPK sgRNA 4 for dox-inducible knockdown

Depositor

Mauro Calabrese

Article

Schertzer et al Mol Cell. 2019 Aug 8;75(3):523-537.e10.

Insert

HNRNPK (Hnrnpk Mouse)

Use

CRISPR

Promoter

U6

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pEF.DEST51-PTPN11(D425A,C459S)-Clover

Plasmid

#215526

Purpose

Expression vector with a clover-tagged phosphatase-inactive Shp2 mutant (D425A,C459S)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN11 (PTPN11 Human)

Tags

Clover

Expression

Mammalian

Mutation

D425A, C459S (Phosphatase inactive)

Promoter

EF1a

Available Since

Sept. 3, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR2b-PTPN11(D425A,C459S)-Clover

Plasmid

#215520

Purpose

Gateway entry vector with a clover-tagged phosphatase-inactive Shp2 mutant (D425A,C459S)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN11 (PTPN11 Human)

Use

Gateway entry vector

Tags

Clover

Mutation

D425A, C459S (Phosphatase inactive)

Promoter

none

Available Since

Sept. 3, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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PB_rtTA_BsmB-HNRNPK_sgRNA_1

Plasmid

#242897

Purpose

PiggyBac cargo vector with HNRNPK sgRNA 1 for dox-inducible knockdown

Depositor

Mauro Calabrese

Article

Schertzer et al Mol Cell. 2019 Aug 8;75(3):523-537.e10.

Insert

HNRNPK (Hnrnpk Mouse)

Use

CRISPR

Promoter

U6

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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