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R6K_tac_dGFPmut3_ChInt

Plasmid

#187388

Purpose

chromosomal integration downstream of glmS gene via Tn7 system of a cassette containing IPTG-inducible destabilized GFPmut3 (containing a AAV C-terminal tail)

Depositor

Roi Avraham

Insert

dGFPmut3_AAV

Tags

AAV

Expression

Bacterial

Mutation

C-terminal AAV tail

Available since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD5-GCaMP6f

Plasmid

#178728

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of GCaMP6f in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

Depositor

Jordane Dimidschstein

Publication

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

GCaMP6f

Use

AAV

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS4-GCaMP6f

Plasmid

#178730

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of GCaMP6f in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Publication

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

GCaMP6f

Use

AAV

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD4-dTom

Plasmid

#178715

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of dTom in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

Depositor

Jordane Dimidschstein

Publication

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

dTom

Use

AAV

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD6-dTom

Plasmid

#178717

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of dTom in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

Depositor

Jordane Dimidschstein

Publication

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

dTom

Use

AAV

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS5-dTom

Plasmid

#178719

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of dTom in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Publication

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

dTom

Use

AAV

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS6-dTom

Plasmid

#178720

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of dTom in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Publication

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

dTom

Use

AAV

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS4-GFP

Plasmid

#178712

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of GFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Publication

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

GFP

Use

AAV

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pMCL9

Plasmid

#176186

Purpose

2 mu cloning vector for single or multiplexed gRNAs, SNR52p-sfGFP-scRNA-SUP4t-CYC1t

Depositor

Florian David

Publication

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Use

Synthetic Biology

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-XI1

Plasmid

#176155

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-1

Depositor

Florian David

Publication

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-XI5

Plasmid

#176158

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-5

Depositor

Florian David

Publication

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-XII2

Plasmid

#176160

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-2

Depositor

Florian David

Publication

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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JPF0454

Plasmid

#124039

Purpose

Encodes pPGK1 driving expression of spCas9 P2A mAzamiGreen in a 2nd gen. Lenti Virus destination vector

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pHR_pPGK1-spCAS9-P2A-mAzamiGreen

Expression

Mammalian

Available since

Feb. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pFH79

Plasmid

#125818

Purpose

Level0 A3A-PBE, wheat codon optimized

Depositor

Vladimir Nekrasov

Publication

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

A3A-PBE, wheat codon optimized

Use

CRISPR and Synthetic Biology

Expression

Plant

Available since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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JPF0446

Plasmid

#124022

Purpose

BxBI Destination vector with H2B::GFP expressed from CAG promoter

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

BXBI_pCAG-H2B::msfGFP-bghpA

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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ARB342

Plasmid

#124025

Purpose

Encodes TU with connectors LS R1 and an A4 insulator, and pEF1a expressing nuclear mAzamiGreen

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

LS-A4_EF1a-NLS::mAzamiGreen-bgPA_R1

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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ARB343

Plasmid

#124026

Purpose

Encodes TU with connectors LS R1 and an A4 insulator, and pEF1a expressing nuclear mRuby2

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

LS-A4_EF1a-NLS::mRuby2-bgPA_R1

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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ARB349

Plasmid

#124032

Purpose

Encodes TU with connectors L2 R3 and an C3 insulator, and pCAG expressing membrane-tagged tagBFP

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

L2-C3_CAG-Lyn::tagBFP-SV40_RE

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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ARB351

Plasmid

#124034

Purpose

Encodes TU with connectors L2 R3 and an C3 insulator, and pCAG expressing membrane-tagged mRuby2

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

L2-C3_CAG-Lyn::mRuby2-SV40_RE

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK0_007

Plasmid

#123927

Purpose

Encodes the AAV GFP dropout destination vector as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

AAV Destination vector

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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